Imaging of hydroperoxide and hydrogen peroxide‐scavenging substances by photon emission

Abstract

An imaging system for the hydroperoxide and hydrogen donor was developed by photon emission from the reactive oxygen species-hydrogen donor-KHCO(3)-MeCHO system. Photon intensity (luminance, cd/m(2)) showed a linear correlation with the concentration of some hydroperoxides [hydrogen peroxide (H(2)O(2)), tert-butyl hydroperoxide and methyl ethyl ketone peroxide] and that of hydrogen donors (catechins and anthocyanins). The linear relationship between photon intensity and concentration was observed in polyphenol-rich samples, such as teas, berries and currants. Photon intensity from the H(2)O(2)/polyphenol-rich sample-KHCO(3)-MeCHO system corresponded with H(2)O(2)-scavenging activity, rather than with polyphenol contents. It is possible to classify hydrogen donor species according to absorption features using spectral analysis (gallic acid, E(max) 645 nm, catechins, E(max) 610 nm; anthocyanins, E(max) 690-700 nm). This method is a simple and sensitive detection system for hydroperoxide- and H(2)O(2)-scavenging substances.

Endothelium-Dependent Vasorelaxation Induced by Black Currant Concentrate in Rat Thoracic Aorta

“…According to quantitative analysis with chemiluminescence, BC concentrate showed antioxidant activity by way of H 2 O 2 -scavenging activity (32). The free radical superoxide is known to interact with and inactivate NO (6).…”

Antioxidant Activity of Black Currant Anthocyanin Aglycons and Their Glycosides Measured by Chemiluminescence in a Neutral pH Region and in Human Plasma

“…Photon Emission Intensity and Anthocyanin Measurement of Plasma. Photon emission (PE) intensity was detected using chargecoupled device (CCD) camera (Hamamatsu Photonics, Hamamatsu, Japan) in the same manner as described in a previous report (8). The reaction mixture contained hydroperoxide (0.5 mL), plasma samples (0.5 mL), and saturated KHCO3 in 356 mmol/L of MeCHO (0.5 mL).…”

“…Recently, we developed a preparative scale isolation of four components of BCA, delphinidin 3-rutinoside (Dp3Rut), delphinidin 3-glucoside (Dp3Glc), cyanidin 3-rutinoside (Cy3Rut), and cyanidin 3-glucoside (Cy3Glc) (9), and these purified components may be useful for the elucidation of their structureactivity relationship. Furthermore, we developed an imaging system for hydroperoxide and hydrogen donors by photon emission (8). This photon emission system was found to be applicable to solid samples regardless of the water solubility (8), and this result suggests the possibility of a direct application for the measurement of biomaterials such as blood and organs.…”

“…Furthermore, we developed an imaging system for hydroperoxide and hydrogen donors by photon emission (8). This photon emission system was found to be applicable to solid samples regardless of the water solubility (8), and this result suggests the possibility of a direct application for the measurement of biomaterials such as blood and organs.…”