Imaging of Lipid Peroxidation-Associated Chemiluminescence in Plants: Spectral Features, Regulation and Origin of the Signal in Leaves and Roots

Abstract

Plants, like most living organisms, spontaneously emit photons of visible light. This ultraweak endogenous chemiluminescence is linked to the oxidative metabolism, with lipid peroxidation constituting a major source of photons in plants. We imaged this signal using a very sensitive cooled CCD camera and analysed its spectral characteristics using bandpass interference filters. In vitro oxidation of lipids induced luminescence throughout the visible spectrum (450–850 nm). However, luminescence in the red spectral domain (>640 nm) occurred first, then declined in parallel with the appearance of the emission in the blue-green (<600 nm). This temporal separation suggests that the chemical species emitting in the blue-green are secondary products, possibly deriving from the red light-emitting species. This conversion did not seem to occur in planta because spontaneous chemiluminescence from plant tissues (leaves, roots) occurred only in the red/far-red light domain (>640 nm), peaking at 700–750 nm. The spectrum of plant chemiluminescence was independent of chlorophyll. The in vivo signal was modulated by cellular detoxification mechanisms and by changes in the concentration of singlet oxygen in the tissues, although the singlet oxygen luminescence bands did not appear as major bands in the spectra. Our results indicate that the intensity of endogenous chemiluminescence from plant tissues is determined by the balance between the formation of luminescent species through secondary reactions involving lipid peroxide-derived intermediates, including singlet oxygen, and their elimination by metabolizing processes. The kinetic aspects of plant chemiluminescence must be taken into account when using the signal as an oxidative stress marker.

hyperspectral classification of plateau zokor molehills at different successional stages in alpine meadows

“…Green plants have signi cant spectral re ectance properties unlike other features, and the re ectance spectra of plants are closely linked to their growth, development and health 24 . Chlorophyll in plants is most responsive to spectra in the visible light band, absorbing light in both the blue (450 nm) and red (650 nm) wavelength ranges and producing an absorption peak near 550 nm 25 . In this study, the raw spectral re ectance of molehills at different successional stages showed that, except for ZM0, all other molehills showed different degrees of "green peaks" around 550 nm, with ZMM, ZM2, CK, and ZM1 in descending order, which was attributed to the fact that ZM0 had a lower vegetative cover, and therefore showed a spectral type closer to the soil.…”

Manganese Nanoparticles: Synthesis, Mechanisms of Influence on Plant Resistance to Stress, and Prospects for Application in Agricultural Chemistry

“…101 LP causes oxidative degradation of lipids in the cell membrane, leads to the oxidation of protein SH groups, and inhibits the synthesis of proteins and enzymes, which provokes cell death. 102 During the LP process, primary and secondary metabolites are formed such as conjugated dienes (CDs) and malonic dialdehyde (MDA), respectively. 103 LP products are toxic compounds that alter the functional properties of biopolymers.…”

“…The interaction of PUFAs with ROS triggers successive LP reactions . LP causes oxidative degradation of lipids in the cell membrane, leads to the oxidation of protein SH groups, and inhibits the synthesis of proteins and enzymes, which provokes cell death . During the LP process, primary and secondary metabolites are formed such as conjugated dienes (CDs) and malonic dialdehyde (MDA), respectively .…”

Determination of the antigenic properties of quinoidr adiotoxin using the indirect hemagglutination test (IHA)

“…The work of V.Y. Horgan et al [4,5,6] revealed the high toxicity of lipid peroxides accumulating in the irradiated organism. Low-molecular toxic substances were found in the blood of irradiated animals and plants [7,8].…”