Imaging of objects through a thin scattering layer using a spectrally and spatially separated reference

Xu, Xiaoqing; Xie, Xiangsheng; Thendiyammal, Abhilash; Zhuang, Huichang; Xie, Junpeng; Liu, Yikun; Zhou, Jianying; Mosk, Allard

doi:10.1364/oe.26.015073

Cited by 47 publications

(15 citation statements)

References 42 publications

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“…Fluorescence is conventionally considered very incoherent, so these techniques based on coherence do not straightforwardly apply. However, it has been shown that it is still possible to reconstruct a fluorescent object hidden behind a scattering medium, by * claudio.moretti@lkb.ens.fr † sylvain.gigan@lkb.ens.fr analyzing spatial correlation within a single low contrast fluorescent speckle [17][18][19][20][21]. These techniques require thin media (with the so-called memory effect [22]) and limited object size [17][18][19], restricting their application at depth in tissues.…”

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confidence: 99%

Readout of fluorescence functional signals through highly scattering tissue

Moretti

Gigan

2020

Nat. Photonics

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Fluorescence is a powerful mean to probe information processing in the mammalian brain [1]. However, neuronal tissues are highly heterogeneous and thus opaque to light. A wide set of noninvasive or invasive techniques for scattered light rejection, optical sectioning or localized excitation, have been developed, but non-invasive optical recording of activity through highly scattering layer beyond the ballistic regime is to date impossible. Here, we show that functional signals from fluorescent time-varying sources located below an highly scattering tissue can be retrieved efficiently, by exploiting matrix factorization algorithms to demix this information from low contrast fluorescence speckle patterns.In the last decades novel light-enabled tools established new paradigms in neuroscience [1][2][3], and among them the emergence of fluorescence functional indicators revolutionized the way to monitor information processing through the brain of different animal models, with unprecedented combination of contrast, resolution and specificity [4,5]. With this approach, optical resolution is often not paramount, and in general only a coarse (cell) resolution is needed [6]. Furthermore, when the location of neurons is known, it is possible to avoid slow rasterscanning techniques and image only the needed location at high frame-rate [6-10].However, brain tissues are usually opaque, and light emitted or delivered at depth in the brain is often quickly subject to multiple scattering events. This results in a loss of directionality after few scattering lengths, corresponding to a few hundred microns, and ultimately means that all wide field or scanning microscopy techniques fails at depth. While the brains of simple organisms are sufficiently small and/or transparent so they can be imaged in totality, for instance C.Elegans, drosophila or zebrafish[5], mammalian brain, starting with its most common animal model, the mouse, is too large and too scattering to image in full. When imaging is performed in superficial layers, it is possible to implement wide field recording with multi-site multiphoton excitation [10,11], or with wide-field excitation and a-posteriori demixing, exploiting the few forward scattered or ballistic photons as a seed to separate the individual neuron contributions [12][13][14]. However, observing neuronal activity beyond a millimeter in the cortex or through the skull, is to date extremely challenging. In this depth range, in the multiple scattering regime, several techniques have been introduced to focus light and image using wavefront shaping [15,16]. Fluorescence is conventionally considered very incoherent, so these techniques based on coherence do not straightforwardly apply. However, it has been shown that it is still possible to reconstruct a fluorescent object hidden behind a scattering medium, by * claudio.moretti@lkb.ens.fr † sylvain.gigan@lkb.ens.fr analyzing spatial correlation within a single low contrast fluorescent speckle [17][18][19][20][21]. These techniques require thin media (with the ...

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confidence: 99%

Readout of fluorescence functional signals through highly scattering tissue

Moretti

Gigan

2020

Nat. Photonics

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“…In the first type, optical signal processing [6][7][8], correlation techniques [9][10][11][12][13] and phase retrieval algorithms [14,15] have been developed to decode a speckle signature and convert it into useful information. A second direction of research is to develop adaptive aberration correction methods to compensate for the disturbance introduced by the scattering medium [16,17].…”

Section: Introductionmentioning

confidence: 99%

A compact single channel interferometer to study vortex beam propagation through scattering layers

Lathika

Anand

Bhattacharya

2020

Sci Rep

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We propose and demonstrate a single channel interferometer that can be used to study how vortex beams propagate through a scatterer. The interferometer consists of a multifunctional diffractive optical element (MDOE) synthesized by the spatial random multiplexing of a Fresnel zone plate and a spiral Fresnel zone plate with different focal lengths. The MDOE generates two co-propagating beams, such that only the beam carrying orbital angular momentum is modulated by an annular stack of thin scatterers located at the focal plane of the Fresnel zone plate, while the other beam passes through the centre of the annulus without any modulation. The interference pattern is recorded at the focal plane of the spiral Fresnel zone plate. The scattering of vortex beams through stacks consisting of different number of thin scatterers was studied using the proposed optical setup. Conflicting results have been reported earlier on whether higher or lower charge beams suffer more deterioration. The proposed interferometer provides a relatively simple and compact means of experimentally studying propagation of vortex beams through scattering medium.

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“…The deconvolution approach, 34,[39][40][41][42][43][44][45][46] instead, can reconstruct the image of the object from its speckle and the PSF of the scattering system 47 in real-time. By harnessing the spatial, spectral properties of the PSF of a scattering system, large Field-of-View (FOV) beyond the limit of the OME, 34,45,46 extended Depth-of-Field (DOF) for 3D imaging, 39 color image reconstruction, 34 or spectral-resolved imaging recovery 40,41 can be realized by a simple deconvolution between the speckle and the PSF. If a priori knowledge of a reference object, 44 spatial-correlation of the speckles, 45 or estimation of the speckle pattern 42 can be attained, noninvasive imaging recovery through a scattering medium can be realized.…”

Section: Introductionmentioning

confidence: 99%

Exploiting the point spread function for optical imaging through a scattering medium based on deconvolution method

Xie

Liu

et al. 2019

J. Innov. Opt. Health Sci.

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Visual perception of humans penetrating turbid medium is hampered by scattering. Various techniques have been prompted recently to recover optical imaging through turbid materials. Among them, speckle correlation based on deconvolution is one of the most attractive methods taking advantage of high imaging quality, robustness, ease-of-use, and ease-of-integration. By exploiting the point spread function (PSF) of the scattering system, large Field-of-View, extended Depth-of-Field, noninvasiveness and spectral resoluation are now available as successful solutions for high quality and multifunctional image reconstruction. In this paper, we review the progress of imaging through a scattering medium based on deconvolution method, including the principle, the breakthrough of the limitation of the optical memory effect, the improvement of the deconvolution algorithm and innovative applications.

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Imaging of objects through a thin scattering layer using a spectrally and spatially separated reference

Cited by 47 publications

References 42 publications

Readout of fluorescence functional signals through highly scattering tissue

Readout of fluorescence functional signals through highly scattering tissue

A compact single channel interferometer to study vortex beam propagation through scattering layers

Exploiting the point spread function for optical imaging through a scattering medium based on deconvolution method

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