A polarization digital holography (DH) using angular multiplexing was developed for extracting the Jones matrix of anisotropy materials in one step. This technique is implemented by adopting an off-axis interferometric configuration connecting two identical CCD cameras. The combined orthogonal 45° beams is split using a nonpolarizing beam splitter to produce the sample and reference beams. Our method yields two angular-multiplexing polarization interferograms simultaneously, in which the orthogonal fringe directions for each interferogram are modulated by two self-installed retro-reflector mirrors. In this case, the spatially resolved Jones matrix parameters of the polarization-sensitive materials can be determined in one step. The basic feasibility of the scheme is verified by measuring the Jones matrices of polarizing optics, a transmitted spatial light modulator, and synthetic mica plates.