Imaging Oxygen Pressure in the Rodent Retina by Phosphorescence Lifetime

Wilson, David F.; Vinogradov, Sergei A.; Grosula, Pavel; Sund, Newman J.; Vacarezza, M. Noel; Bennett, Jean

doi:10.1007/0-387-29540-2_19

Cited by 9 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This probe format is versatile as it may be added to aqueous materials, including those for microfluidic cell culture. Water-soluble probes have been primarily used for in vivo biological imaging [49,52,83,102,103,105,107,108,136], but they could potentially be applied to other aqueous environments.…”

Section: Oxygen Sensor Formatsmentioning

confidence: 99%

“…Because LED sources are inexpensive and may be pulsed or modulated, they are very commonly used as excitation sources for optical oxygen sensors [11,12,28,36,38,46,50,54,55,59,60,62,63,78,87, 91,107,108,130], and excitation spectra which overlap well with LED emission spectra are considered an advantage of many indicator compounds such as Pt- and PdOEPK [61]. For excitation spectrum versatility and compatibility with other systems such as fluorescent microscopes, filtered broader-spectrum sources such as Xenon flash lamps and mercury-arc lamps have also been used [46–51,52,56,83,100–103,105,136].…”

Section: Optical Measurement Systemsmentioning

confidence: 99%

“…The most commonly used detectors for this application are CCDs [28,38,39,46–48,56,62,63,91,100,103,108,130,136], but phototransistor arrays [77] have also been successfully used.…”

Section: Optical Measurement Systemsmentioning

confidence: 99%

See 2 more Smart Citations

Optical Oxygen Sensors for Applications in Microfluidic Cell Culture

Grist

Chrostowski

Cheung

2010

Sensors

149

107

View full text Add to dashboard Cite

The presence and concentration of oxygen in biological systems has a large impact on the behavior and viability of many types of cells, including the differentiation of stem cells or the growth of tumor cells. As a result, the integration of oxygen sensors within cell culture environments presents a powerful tool for quantifying the effects of oxygen concentrations on cell behavior, cell viability, and drug effectiveness. Because microfluidic cell culture environments are a promising alternative to traditional cell culture platforms, there is recent interest in integrating oxygen-sensing mechanisms with microfluidics for cell culture applications. Optical, luminescence-based oxygen sensors, in particular, show great promise in their ability to be integrated with microfluidics and cell culture systems. These sensors can be highly sensitive and do not consume oxygen or generate toxic byproducts in their sensing process. This paper presents a review of previously proposed optical oxygen sensor types, materials and formats most applicable to microfluidic cell culture, and analyzes their suitability for this and other in vitro applications.

show abstract

Section: Oxygen Sensor Formatsmentioning

confidence: 99%

Section: Optical Measurement Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Optical Oxygen Sensors for Applications in Microfluidic Cell Culture

Grist

Chrostowski

Cheung

2010

Sensors

149

107

View full text Add to dashboard Cite

show abstract

“…The phosphorescence intensity output of the iPOB was processed to calculate the phosphorescence lifetime decay (τ), as previously reported (Lo et al, 1996). Phosphorescent lifetime decay of Pd-BPD correlates directly to the oxygen concentration of its surrounding environment (Vinogradov et al, 2003; Wilson et al, 2006). …”

Section: Resultsmentioning

confidence: 99%

Integrated phosphorescence-based photonic biosensor (iPOB) for monitoring oxygen levels in 3D cell culture systems

Rivera

Pozdin

Young

et al. 2019

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Physiological processes, such as respiration, circulation, digestion, and many pathologies alter oxygen concentration in the blood and tissue. When designing culture systems to recapitulate the in vivo oxygen environment, it is important to integrate systems for monitoring and controlling oxygen concentration. Herein, we report the design and engineering of a system to remotely monitor and control oxygen concentration inside a device for 3D cell culture. We integrate a photonic oxygen biosensor into the 3D tissue scaffold and regulate oxygen concentration via the control of purging gas flow. The integrated phosphorescence-based oxygen biosensor employs the quenching of palladium-benzoporphyrin by molecular oxygen to transduce the local oxygen concentration in the 3D tissue scaffold. The system is validated by testing the effects of normoxic and hypoxic culture conditions on healthy and tumorigenic breast epithelial cells, MCF-10A cells and BT474 cells, respectively. Under hypoxic conditions, both cell types exhibited upregulation of downstream target genes for the hypoxia marker gene, hypoxia-inducible factor 1α (HIF1A). Lastly, by monitoring the real-time fluctuation of oxygen concentration, we illustrated the formation of hypoxic culture conditions due to limited diffusion of oxygen through 3D tissue scaffolds.

show abstract

“…The microsensor measures oxygen in the body based on the principle of phosphorescence quenching of metalloporphyrins, a well-established technique with excellent sensitivity and specificity to physiologic oxygen [14–17]. The pHEMA hydrogel is biocompatible, has good oxygen permeability, excellent mechanical properties, and a long history of use in medical devices [18].…”

Section: Methodsmentioning

confidence: 99%

Tissue-Integrating Oxygen Sensors: Continuous Tracking of Tissue Hypoxia

Wisniewski

Nichols

Gamsey

et al. 2017

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

We describe a simple method of tracking oxygen in real-time with injectable, tissue-integrating microsensors. The sensors are small (500 μm × 500 μm × 5 mm), soft, flexible, tissue-like, biocompatible hydrogels that have been shown to overcome the foreign body response for longterm sensing. The sensors are engineered to change luminescence in the presence of oxygen or other analytes and function for months to years in the body. A single injection followed by noninvasive monitoring with a hand-held or wearable Bluetooth optical reader enables intermittent or continuous measurements. Proof of concept for applications in high altitude, exercise physiology, vascular disease, stroke, tumors, and other disease states have been shown in mouse, rat and porcine models. Over 90 sensors have been studied to date in humans. These novel tissueintegrating sensors yield real-time insights in tissue oxygen fluctuations for research and clinical applications.

show abstract

Imaging Oxygen Pressure in the Rodent Retina by Phosphorescence Lifetime

Cited by 9 publications

References 13 publications

Optical Oxygen Sensors for Applications in Microfluidic Cell Culture

Optical Oxygen Sensors for Applications in Microfluidic Cell Culture

Integrated phosphorescence-based photonic biosensor (iPOB) for monitoring oxygen levels in 3D cell culture systems

Tissue-Integrating Oxygen Sensors: Continuous Tracking of Tissue Hypoxia

Contact Info

Product

Resources

About