Imaging Soil Bacteria in an Environmental Scanning Electron Microscope

Principles and application of laser light scattering (LLS) in characterization of the spatial configuration of microbial products in aqueous solution

“…The dimensions of EPS-containing structures around the cells can only be obtained by conventional electron microscopy by using antibodies or lectins as the stabilization aid and staining of EPS with dyes [21]. Operated under a moderate vacuum and without requring sample drying, wet-mode environmental scanning electron microscopy (ESEM) also provides a suitable alternative for imaging microbial products [22,23].…”