Imbalance of Interleukin-17+ T-cell and Foxp3+ Regulatory T-cell Dynamics in Rat Periapical Lesions

Yang, Shasha; Zhu, Lingxin; Xiao, Lan; Shen, Ya; Wang, Li; Peng, Bin; Haapasalo, Markus

doi:10.1016/j.joen.2013.09.033

Cited by 48 publications

(66 citation statements)

References 36 publications

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“…According to this, it could be concluded that the time between day 7 to day 21 is the active phase (rapid development of apical periodontitis), while that between day 28 to day 35 is the stable phase. These results agrees with the previous studies (Liu & Peng, 2013;Yang, et al, 2014), indicating that this model is reliable and effective for apical periodontitis research.…”

Section: Discussionsupporting

confidence: 93%

“…The raw data were analysed by the three-dimensional (3D) image analysis software (VGStudio MAX, Heidelberg, Germany) to measure the volume of the periapical region as previously described (Yang et al, 2014). All measurements were performed in a double blind manner by two trained independent observers.…”

Section: High-resolution X-ray Imaging and µCt Analysismentioning

confidence: 99%

“…Immunohistochemical staining was performed as previously described (Yang, et al, 2014). Briefly, sections were dewaxed in xylene, then rehydrated in graded ethanol (100%, 90%, and 70%), and washed by distilled water.…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Dissecting the Role of Sphingosine 1-Phosphate - Sphingosine 1-Phosphate Receptor 1 in Inflammatory Bone Remodelling

Xiao¹

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KeywordsOsteoimmunology, bone remodelling, osteoclastogenesis, osteogenesis, sphingosine-1-phosphate (S1P), sphingosine-1-phosphate receptor 1 (S1PR1), receptor activator of nuclear factor factor-kappa B ligand (RANKL), macrophages, mesenchymal stromal cells (MSCs), osteogenic differentiation, infection, inflammation, bone loss, apical periodontitis. Sphingosine-1-phosphate (S1P), together with its receptor, sphingosine-1-phosphate receptor 1 (S1PR1), acts as a regulator of both the immune response and bone remodelling processes and, therefore, plays a vital role in osteoimmunology.

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Section: Discussionsupporting

confidence: 93%

Section: High-resolution X-ray Imaging and µCt Analysismentioning

confidence: 99%

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Dissecting the Role of Sphingosine 1-Phosphate - Sphingosine 1-Phosphate Receptor 1 in Inflammatory Bone Remodelling

Xiao¹

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“…8 Th17 play critical roles in the progression of autoimmunity and inflammation by the production of IL-17 and require specific cytokines for their differentiation, such as the transforming growth factor-β (TGF-β), which may be combined with interleukin-6 (IL-6) or IL-21 and the transcription factor RORγt. 9 The participation of Treg and Th17 in the inflammatory response may help explain many unresolved issues in the immunobiology of periapical lesions, such as bone resorption occurring in different manners as a reflection of intracanal infection in similar cases of pulpal necrosis. 10 However, the up or down regulation of both Foxp3 and Th17 in periapical inflammatory disease has been explored mostly in animal models, 1,9,11 with few studies conducted in humans, in different types of chronic lesions, such as radicular cysts or periapical granuloma.…”

Section: Introductionmentioning

confidence: 99%

“…9 The participation of Treg and Th17 in the inflammatory response may help explain many unresolved issues in the immunobiology of periapical lesions, such as bone resorption occurring in different manners as a reflection of intracanal infection in similar cases of pulpal necrosis. 10 However, the up or down regulation of both Foxp3 and Th17 in periapical inflammatory disease has been explored mostly in animal models, 1,9,11 with few studies conducted in humans, in different types of chronic lesions, such as radicular cysts or periapical granuloma. Thus, defining the role of Treg/Th17 is crucial for better understanding the immune system regulation of these lesions.…”

Section: Introductionmentioning

confidence: 99%

Treg and Th17 cells in inflammatory periapical disease: a systematic review

et al. 2017

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The process involved in periapical lesions, which occur as an outcome of pulpal necrosis, is regulated by the immune system including regulatory T cells (Treg) and T helper 17 cell (Th17) responses. The objective of this study was to conduct a frequency systematic review to determine the presence of Treg/Th17 responses and the influence of these cells in the progression of chronic inflammatory periapical lesions in humans. A systematic computerized search was carried out in Pubmed, Medline, Web of Science and Scopus electronic databases from their date of inception through the first week of May 2017. In addition, the reference lists of the included articles and the grey literature were hand-searched. Articles that evaluated the presence and influence of Treg/Th17 in the progression of human periapical lesions were included. Study selection and the quality assessment of the included articles (using the Newcastle-Ottawa scale) were carried out by two authors. Fifty-seven titles/abstracts were screened and eight studies met the eligibility criteria and were included in this systematic review. The included studies showed large variation in the type of periapical lesion assessed, mean age, age range, type of experiment and findings regarding the participation of Th17 and Treg in the status of inflammatory periapical lesions. The studies showed the involvement of Treg in the modulation of the inflammatory response in radicular cysts and periapical granulomas. This systematic review highlights the relationship between Treg and Th17 acting in a subtle balance inhibiting or promoting the progression of human periapical lesions.

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Exosomal miR‐205‐5p derived from periodontal ligament stem cells attenuates the inflammation of chronic periodontitis via targeting XBP1

Miao

Jin

et al. 2022

Immunity Inflam & Disease

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Introduction Chronic periodontitis (CP) is an inflammatory periodontal disease with high incidence and complex pathology. This research is aimed to investigate the function of exosomal miR‐205‐5p (Exo‐miR‐205‐5p) in CP and the underlying molecular mechanisms. Method Exo‐miR‐205‐5p was isolated from miR‐205‐5p mimics‐transfected periodontal ligament stem cells (PDLSCs), and subsequently cocultured with lipopolysaccharide (LPS)‐induced cells or injected into LPS‐treated rats. The mRNA expression of inflammatory factors and Th17/Treg‐related factors were measured by quantitative real‐time PCR. The contents of inflammatory factors and the percentages of Th17/Treg cells were measured by enzyme‐linked immunosorbent assay and flow cytometry, respectively. Besides, the target relation between miR‐205‐5p and X‐box binding protein 1 (XBP1) was explored. Results MiR‐205‐5p was downregulated in LPS‐induced PDLSCs and corresponding exosomes. Exo‐miR‐205‐5p inhibited inflammatory cell infiltration, decreased the production of TNF‐α, IL‐1β, and IL‐6, and decreased the percentage of Th17 cells in LPS‐treated rats. In addition, XBP1 was a target of miR‐205‐5p. Overexpression of XBP1 weakened the effects of Exo‐miR‐205‐5p on inhibiting inflammation and regulating Treg/Th17 balance in LPS‐induced cells. Conclusions Exo‐miR‐205‐5p derived from PDLSCs relieves the inflammation and balances the Th17/Treg cells in CP through targeting XBP1.

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Imbalance of Interleukin-17+ T-cell and Foxp3+ Regulatory T-cell Dynamics in Rat Periapical Lesions

Cited by 48 publications

References 36 publications

Dissecting the Role of Sphingosine 1-Phosphate - Sphingosine 1-Phosphate Receptor 1 in Inflammatory Bone Remodelling

Dissecting the Role of Sphingosine 1-Phosphate - Sphingosine 1-Phosphate Receptor 1 in Inflammatory Bone Remodelling

Treg and Th17 cells in inflammatory periapical disease: a systematic review

Exosomal miR‐205‐5p derived from periodontal ligament stem cells attenuates the inflammation of chronic periodontitis via targeting XBP1

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