Iminodiacetic acid-modified magnetic poly(2-hydroxyethyl methacrylate)-based microspheres for phosphopeptide enrichment

Ti4+-phosphate functionalized cellulose for phosphopeptides enrichment and its application in rice phosphoproteome analysis

“…In this technique, metal ions, such as Fe 3+ [18], Ga 3+ [19,20], Zr 4+ [21], and Ti 4+ [22], are immobilized on the supporting matrix via chelating ligands, such as IDA [16] and NTA [23]. The principle of phosphopeptides enrichment is based on electrostatic interactions between positively charged metal ions and negatively charged phosphate groups of phosphopeptides.…”

“…To circumvent this obstacle, enrichment approaches of phosphopeptides before MS analysis are indispensable. Among the techniques, IMAC [12][13][14][15][16] has drawn lots of attentions and has been the most commonly used one.…”

New Magnetic Graphitized Carbon Black TiO2 Composite for Phosphopeptide Selective Enrichment in Shotgun Phosphoproteomics

“…For the same interval, the percentage drops to 11% for the developed mGCB@TiO 2 composite, which also coenriched a larger amount of more hydrophilic peptides, with 40% of them having a GRAVY ≤ −1 vs only 19% for the commercial spin column. This consideration, together with recent findings supporting that a hydrophilic phase would improve enrichment by reducing unspecific binding, ,, could be accounted for the better selectivity of the composite than pure TiO 2 .…”

“…For the same interval, the percentage drops to 11% for the developed mGCB@TiO 2 composite, which also coenriched a larger amount of more hydrophilic peptides, with 40% of them having a GRAVY ≤ −1 vs only 19% for the commercial spin column. This consideration, together with recent findings supporting that a hydrophilic phase would improve enrichment by reducing unspecific binding, 31,39,40 could be accounted for the better selectivity of the composite than pure TiO 2 . Some differences were observed for the molecular weight distribution, where the commercial spin column preferentially isolated smaller peptides (<2500 Da), with 49% vs 41% and 67% vs 56%, respectively, for the identified phosphopeptides and coisolated peptides (Figure 3b).…”

Application of heptapeptides containing D‐amino acid residues immobilized to magnetic particles and Sepharose for the study of binding properties of gastric aspartic proteases

“…in application of immobilized enzymes esp. of proteinases for the preparation of peptide maps in IMAC (immobilized metal ion affinity chromatography) separation applied for phosphoprotein and for phosphopeptide enrichment , specific protein purification for high sensitive mass spectrometric screening procedures . The combination of magnetic and affinity separation has received considerable attention in recent years and represents a perspective separation technique.…”