Imipenem resistance in clinical Escherichia coli from Qom, Iran

Shams, Saeed; Hashemi, Ali; Esmkhani, Mohammad; Kermani, Somayeh; Shams, Elham; Piccirillo, Alessandra

doi:10.1186/s13104-018-3406-6

Cited by 23 publications

(19 citation statements)

References 35 publications

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“…In our study, the highest and lowest resistance rates were observed against imipenem (56.1%) and fosfomycin (15%), respectively. Previously, a survey reported a 3% rate of resistance to imipenem in Iran [25], which is much lower than the rate observed in our study. However, the survey included isolates from both blood and urine samples.…”

Section: Discussioncontrasting

confidence: 92%

Molecular Monitoring of Fosfomycin Resistance in Escherichia coli Strains Isolated from Patients with Urinary Catheters in north-east of Iran

Kolbadinezhad.

Fozouni

2018

JoMMID

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INTRODUCTION Urinary tract infection (UTI) is a common infectious disease that could be either symptomatic or asymptomatic. Urinary catheters and pathogens emanating from the gastrointestinal tract are the leading causes of UTI [1]. Uropathogenic Escherichia coli (UPEC) is responsible for 70-90% of UTIs in humans. Therefore, early and accurate diagnosis of UTI is crucial for effective treatment and prevention of infection spread to the upper urinary tract [2]. Prevalence of multi-drug resistant E. coli strains that are resistant to beta-lactams, fluoroquinolones, and aminoglycosides have increased dramatically in recent years. As a bactericidal antibiotic, fosfomycin is used with different formulations for the treatment of complicated UTIs. Despite being an old-generation antibiotic, fosfomycin has recently played a significant role in controlling resistant bacteria, particularly E. coli [3, 4]. This antibiotic is a phosphoenolpyruvate analog that binds to UDP-N-acetylglucosamine enolpyruvyl transferase (MurA), (an essential enzyme for peptidoglycan biosynthesis) leading to bacterial cell lysis and death [5]. Fosfomycin can also inhibit this enzyme by covalently binding to a key cysteine residue (115 in E. coli) in the active site of MurA [6]. The E. coli MurA with the Cys115 to Asp mutation exhibits full enzymatic activity and makes the bacteria resistance to fosfomycin, while the gene with the Cys115 to Glu mutation shows no activity [7]. Glucose-6-phosphate (G6P) transporter, UhpT and glycerol-3-phosphate (G3P) transporter (GlpT) facilitate the incorporation of fosfomycin into the bacterial cells [8]. G3P induces expression of GlpT in E. coli. In these bacteria, two domains in the GlpT structure are attached to the central loop and act as a secondary active transporter to transfer substrates into the cytoplasm. However, mutations in the glpT, GlpT transporter, and murA may decrease fosfomycin susceptibility and uptake. Expression of the GlpT and UhpT transporters is induced by their substrates, G3P and G6P, respectively, and requires the presence of cAMP. Mutations in the structural genes involved in these pathways lower the antibiotic uptake, thereby conferring different levels of fosfomycin resistance [9]. In this study, we aimed to investigate the genetic pattern of fosfomycin resistance in UPEC isolates from patients with UTI. MATERIAL AND METHODS Patients and bacterial isolation. Urine samples were collected from 106 patients with UTI in three hospitals of Introduction: Urinary tract infection is a common nosocomial infection that has recently become difficult to treat because of the increased emergence of multi-drug resistant strains. This study aims to determine the minimum inhibitory concentration, and molecular pattern of resistance to fosfomycin in Escherichia coli isolates originated from patients hospitalized with urinary tract infection in the intensive care unit (ICU) and coronary care unit (CCU). Methods: Urine samples were obtained from 106 patients in three hospitals of Gorgan, northeast of Ir...

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Section: Discussioncontrasting

confidence: 92%

Molecular Monitoring of Fosfomycin Resistance in Escherichia coli Strains Isolated from Patients with Urinary Catheters in north-east of Iran

Kolbadinezhad.

Fozouni

2018

JoMMID

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“…In positive cases of Shigella spp. and Salmonella spp., an agglutination reaction was observed between the antigen and antibody (12)(13)(14). Other biochemical methods (e.g., esculin hydrolysis, DNase, coagulase, catalase, hemolysis, growth on 6.5% NaCl, and susceptibility to optochin) were also used for the detection of the Gram-positive bacteria (15,16).…”

Section: Isolation and Detection Of The Bacteriamentioning

confidence: 99%

Identification of Bacteria Contaminating Musca domestica (Diptera: Muscidae) Collected from Animal Husbandries

et al. 2020

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Background: House flies (Musca domestica L.) may mechanically transmit many microorganisms to humans. Objectives: To identify bacteria contaminating Musca domestica (Diptera: Muscidae) collected from animal husbandries in province of Qom, Iran in the year of 2019. Methods: In the cross-sectional study, house flies were captured by plastic water bottle fly traps and insect nets from four districts of the Qom Province and were immediately transferred to the Department of Medical Entomology, School of Public Health in sterile glass containers, individually. The bacterial isolation of the surface and gut contents of the flies were separately performed by biochemical methods. To confirm Salmonella spp. and Shigella spp., antigen-antibody agglutination reactions were carried out by specific antisera. Results: A total of 23 bacterial species were identified from 160 house fly external surfaces and/or digestive tract were isolated. The most frequently isolated bacteria from the external body and the digestive tract of 160 flies were E. coli (73.8%) and P. aeruginosa (100%), respectively. The most frequently isolated bacteria were P. aeruginosa, Salmonella serogroup D, S. dysenteriae, E. coli, C. freundii, S. aureus, and S. epidermidis. Conclusions: House flies are important in the mechanical transmission of a different range of the pathogens. In addition, because of the geographical features of Qom and isolated pathogens, it seems that the possibility of mechanical disease transmission by the flies can be increased.

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“…In this study, 80.9% of the urinary isolates of E. coli were MDR, which was very similar to the study performed in Ahwaz (22). In the research of Badamachi et al, (18), 54.6% of urinary isolates were MDR, while only 1 to 5-year-old children were studied. Since the prevalence of MDR E. coli strains, especially in burn patients, can increase morbidity and mortality rates (29), so their presence can be a major risk factor for the patients.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the antimicrobial resistance patterns and MDR frequency in Escherichia coli strains isolated from clinical specimens taken from patients in Iran: 2017–2019

Bazgir¹,

Gholami²,

Goli³

2020

Preprint

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Objectives The purpose of this study was to evaluate the antimicrobial resistance and frequency of MDR strains in E. coli isolated from patients in two hospitals of Iran. In this descriptive-analytical study, 13322 clinical specimens were collected from two teaching hospitals. The E. coli isolates were identified using standard bacteriological methods. Then, the resistance patterns of the isolates were analyzed by disk agar diffusion method according to the CLSI.Results Out of 13322 clinical samples, 964 (7.23%) isolates of E. coli were identified. In both hospitals, high resistance to ampicillin and cefalexin was presented in 621 (64.4%) and 402 (41.7%) isolates, respectively. The highest antibiotic resistance was observed in burn unit, burn ICU and burn restoration section, while all 8 strains which were isolated from the neonatal-ICU, were sensitive to all tested antibiotics except cefalexin, nitrofurantoin, nalidixic acid, and ampicillin. Also, the bacteria isolated from urine, wound, stool, and blood samples were resistant to all tested antibiotics. Increased resistance to different antibiotics in burn hospital has created increasing concern. Very high resistance to some antibiotics indicates that these drugs are misused in therapeutic centers and highlights that infection control measures should be arranged in the ICUs of our hospitals.

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Imipenem resistance in clinical Escherichia coli from Qom, Iran

Cited by 23 publications

References 35 publications

Molecular Monitoring of Fosfomycin Resistance in Escherichia coli Strains Isolated from Patients with Urinary Catheters in north-east of Iran

Molecular Monitoring of Fosfomycin Resistance in Escherichia coli Strains Isolated from Patients with Urinary Catheters in north-east of Iran

Identification of Bacteria Contaminating Musca domestica (Diptera: Muscidae) Collected from Animal Husbandries

Evaluating the antimicrobial resistance patterns and MDR frequency in Escherichia coli strains isolated from clinical specimens taken from patients in Iran: 2017–2019

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