Immature Citrus Fruit Extracts Enhance the Apoptosis Inducing Potential of Cisplatin in Human Malignant Melanoma A375 Cells via Regulation of Nitric Oxide and Inhibitor of Apoptosis Family (IAP)

Kim, Ji Hye; Kim, Min Young

doi:10.5352/jls.2014.24.4.454

Cited by 4 publications

(1 citation statement)

References 36 publications

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“…For example, Citrus polyphenol limonene promoted NO• production in several studies, leading to an inhibition in proliferation of human melanoma A375 cells [22], leukemia HL-60 cells [23], and epidermoid carcinoma A431 cells [24]. On the other hand, Sudachitin, a polymethoxyflavone from Citrus sudachi, inhibited LPS-mediated NO• production and iNOS protein expression in RAW264.7 macrophages [9], and Citrus nobiletin reduced NO• levels and decreased iNOS expression in hepatocytes [25].…”

Section: Discussionmentioning

confidence: 99%

Apoptotic properties of Citrus sudachi Hort, ex Shirai (Rutaceae) extract on human A549 and HepG2 cancer cells

Kim¹,

Kim²

2016

Trop. J. Pharm Res

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Purpose: To investigate whether Citrus sudachi harvested at two stages of maturity can induce toxicity in a cell-specific manner and to determine the possible mechanisms of Citrus sudachi-induced cytotoxic responses in two types of cancer cells (human lung adenocarcinoma A549 and hepatocellular carcinoma HepG2 cells) and two normal cell lines (lung 16HBE140-and liver CHANG cells). Methods: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and annexin V/propidium iodidle assay were used to test the antiproliferative activity and apoptosis of methanol extract of Citrus sudachi, respectively. Griess reaction and reverse transcriptase-polymerase chain reaction (RT-PCR) were carried out to evaluate nitric oxide (NO • ) production and the mRNA levels of inhibitors of apoptosis (IAP). Results: Citrus sudachi exerted cytotoxicity in a time-dependent manner in cancer cells which increased with increase in maturity but did not affect normal cells. Citrus sudachi was found to induce accumulation of cells in the sub

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Section: Discussionmentioning

confidence: 99%

Apoptotic properties of Citrus sudachi Hort, ex Shirai (Rutaceae) extract on human A549 and HepG2 cancer cells

Kim¹,

Kim²

2016

Trop. J. Pharm Res

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Endogenous Nitric Oxide Strengthens Doxorubicin-induced Apoptosis in Human Colorectal Cell Lines

Im¹,

Kim²,

Kim³

2014

Journal of Life Science

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Doxorubicin is a general chemotherapy drug widely used for a number of cancers. However, the correlation between endogenous nitric oxide (NO • ) levels and chemoresistance to doxorubicin remains unclear. In this study, we investigated the effect of endogenous NO• on the anticancer activity of doxorubicin in human colon cancer cell lines HCT116 and HT29 with different p53 status. The cells were treated with either doxorubicin alone or in combination with the NO• synthase (NOS) inhibitor N G -monomethyl-L-arginine (NMA). Doxorubicin differentially inhibited the growth of both the HCT116 (p53-WT) and HT29 (p53-MUT) cells, which was mitigated by cotreatment with NMA. Further studies revealed that inhibition of endogenous NO• mitigated doxorubicin-induced apoptosis in the HCT116 and HT29 cells, as evidenced by apoptotic DNA fragmentation and the sub-G1 peak of apoptotic markers. Apoptosis was delayed in the HT29 cells, and its magnitude was greatly reduced, underscoring the importance of the modulation of p53 in the response. RT-PCR analysis revealed that doxorubicin down-regulated levels of inhibitors of the apoptosis family (cellular IAP-1 and -2). Collectively, these data show that induction of apoptosis by doxorubicin in human colon cancer cells is possibly related to modulation of endogenous NO• , the expression of the IAP family of genes, and the status of p53. The underlying mechanisms may represent potential targets for adjuvant strategies to improve the efficacy of chemotherapy for colon cancer.

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Apoptotic Effect of Sasa quelpaertensis Nakai in Human Colon Cancer HT-29 Cells

Byun¹,

Kim

2014

Journal of Life Science

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Sasa quelpaertensis Nakai (Korean name, Jeju-Joritdae) is one of the most abundant plants on Mt. Halla, Jeju Island, and it has long been used in traditional medicines. Recent studies have reported it as possessing various beneficial functions, including anti-inflammatory, anti-diabetic, anti-hypertension, anti-gastritis, anti-oxidant, and anti-cancer effects. However, the molecular mechanisms of its anti-cancer activity have not been clearly elucidated. In this study, we investigated the anti-cancer effects and mechanism of S. quelpaertensis on human colon cancer HT-29 cells. Cell growth inhibition by S. quelpaertensis was determined by MTT assay. Apoptosis was performed by DNA fragmentation, flow cytometry with propidium iodide staining (PI), and reverse transcription-polymerase chain reaction (RT-PCR) to confirm the anti-apoptotic factors, such as inhibitor of apoptosis (IAP) family members. NO

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Immature Citrus Fruit Extracts Enhance the Apoptosis Inducing Potential of Cisplatin in Human Malignant Melanoma A375 Cells via Regulation of Nitric Oxide and Inhibitor of Apoptosis Family (IAP)

Cited by 4 publications

References 36 publications

Apoptotic properties of Citrus sudachi Hort, ex Shirai (Rutaceae) extract on human A549 and HepG2 cancer cells

Apoptotic properties of Citrus sudachi Hort, ex Shirai (Rutaceae) extract on human A549 and HepG2 cancer cells

Endogenous Nitric Oxide Strengthens Doxorubicin-induced Apoptosis in Human Colorectal Cell Lines

Apoptotic Effect of Sasa quelpaertensis Nakai in Human Colon Cancer HT-29 Cells

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