Immobilised heparin accelerates the healing of human wounds in vivo

Abstract

Locally produced growth factors are of great importance in wound healing in human skin. Wound fluid from chronic wounds contains low concentrations of growth factors possibly because of rapid degradation as a result of the high concentration of proteases. Many growth factors involved in wound healing bind to heparin and are thereby stabilised and activated. We have recently shown that heparin in combination with chitosan stimulates re-epithelialisation in an in vitro model of human wound healing. In the present study we investigated the effects of a chitosan-heparin membrane on wound healing in 10 split-thickness graft donor sites in human skin. The chitosan-heparin membrane stimulated healing of the donor sites both when judged macroscopically in a blinded fashion and when biopsy specimens from the treated and untreated parts of the wound were investigated microscopically. We hypothesise that the beneficial effects of the chitosan-heparin membrane result from slow release of heparin into the wound area which protects locally produced growth factors. The result is increased stabilisation and concentration of growth factors in the wound area, which stimulate healing. We believe that these results may be important in the treatment of wounds that are reluctant to heal.

Maintenance of CD34 Expression During Proliferation of CD34 + Cord Blood Cells on Glycosaminoglycan Surfaces

“…We have previously used this ionic interaction to microencapsulate primary hepatocytes [15,22]. Similar chitosan-GAG complexes have also been applied as aids to wound healing and tissue regeneration [23][24][25]. Membranes produced by the GAG-chitosan complex formation procedure appeared granular and textured under the phase contrast light microscope.…”

Chitosan as a starting material for wound healing applications

“…This confirms the importance of porosity when designing scaffolds for wound healing. In addition, Kratz et al (Kratz et al, 1998) studied the effect of chitosan-heparin membrane on wound healing in human skin. Chitosanheparin complex showed faster and complete re-epithelialization after 12 days, compared to the donor untreated sides where an incomplete re-epithelialization was observed even after 15 days.…”

“…Rapid wound healing in human subjects (Azad et al, 2004;Stone et al, 2000) Membranes Shortens re-epithelialization in human subjects (Kratz et al, 1998) …”

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“…Увеличение числа гнойных заболеваний и послеоперационных осложнений, учащение случаев генерализации инфекции и различного рода токсико-ал-лергических реакций, свидетельствует о нерешенности проблемы гнойной инфекции в хирургии. В связи с этим дальнейшее совершенствование методов лечения острых гнойных заболеваний мягких тканей и их осложнений остается одним из актуальных направлений современной практической хирургии [14,31,44,52,57].…”

“…Доказано, что мутации гена SCN5A, приводящие к замене некоторых аминокислот натриевого канала, влияют на развитие синдрома Бругада и синдрома внезапной ночной сердечной смерти. Эти гетерогенные заболевания схожи по клинической и ЭКГ картине и обусловлены нарушением тока ионов натрия или нарушением образования натриевых ионных каналов, что приводит к анормальному сердечному ритму [30,52]. Мутации в виде делеции или инсерции нуклеотидов в гене SCN5A были зафиксированы у пациентов с синдромом удлиненного интервала QT 3 типа.…”