Immobilised Lipase for In Vitro Lipolysis Experiments

Comparison of bulk and microfluidic methods to monitor the phase behaviour of nanoparticles during digestion of lipid-based drug formulations using in situ X-ray scattering

“…In both microfluidic devices, the point of complete mixing occurred sufficiently early in the channels that it did not impede phase transitions. Additionally, factors such as temperature and pH, which were previously controlled in the pH-stat approach and absent in either microfluidic configuration, were found not to exert a significant influence on digestion kinetics which was also a factor concluded by Phan et al 39 The OTS-MF device could identify phase transitions, however there were inconsistencies in the transition behaviour between different TFR. The transitions were not consistent when changing the flow rate to account for the limited capillary length.…”

Caco-2 Cell Conditions Enabling Studies of Drug Absorption from Digestible Lipid-Based Formulations

“…A modified in vitro digestion model employing this enzyme has been developed previously, showing that a similar extent of digestion could be obtained for the digestion of Captex 355 and Tricaprylin provided that the digestion lasts long enough. The activity of the immobilized lipase was shown to be independent of buffer or pH, enabling its use in protocols mimicking lipid digestion in different segments of the gastrointestinal tract ( 35 ). Other advantages are that immobilized lipase (i) enables easy separation from the digestion medium (ii) is reusable and, (iii) shows increased thermal stability.…”

“…This difference may be due to the specificity and affinity of the enzymes. The immobilized lipase consists of only one kind of lipase, whereas pancreatic extract contains a mixture of enzymes including phospholipase A2, colipase, and pancreatic lipase-related protein ( 35 ). In addition, access of the active site of immobilized lipase to triglycerides might be limited.…”

How relevant are assembled equilibrium samples in understanding structure formation during lipid digestion?

“…HPLC with refractive index detection was employed to separate and quantify fatty acids during digestion for correlation with total fatty acid produced as indicated by the pH stat titration, as previously described [23]. Briefly, an isocratic reversed-phase HPLC method using a 4.6 Â 150 mm Phenomenex Luna C 8 (2) (5 lm, 100 Å), with a 15 Â 3 mm Brownlee RP-18 (7 lm) guard column was used.…”