Immobilization and Release of Platelet-Rich Plasma from Modified Nanofibers Studied by Advanced X-ray Photoelectron Spectroscopy Analyses

Manakhov, Anton; Permyakova, Elizaveta S.; Solovieva, Anastasiya O.; Sitnikova, Natalya A.; Kiryukhantsev-Korneev, Ph. V.; Konopatsky, Anton S.; Shtansky, Dmitry V.

doi:10.3390/polym15061440

Cited by 6 publications

(2 citation statements)

References 43 publications

(51 reference statements)

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“…All these findings suggested that PRP was significantly incorporated into our layers. Our special curve fitting presented in previous work revealed that more than 50% of the surface is covered by PRP [26]. Hence, the surface of the nanofibers was successfully modified with the PRP proteins.…”

Section: Optimization Of Pcl Nanofibers Structure and Prp Immobilizationmentioning

confidence: 63%

PRP of T2DM Patient Immobilized on PCL Nanofibers Stimulate Endothelial Cells Proliferation

Solovieva

Sitnikova

Nimaev

et al. 2023

IJMS

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Diabetic foot ulcers (DFU) are a common complication of Type 2 Diabetes Mellitus (T2DM). Development of bioactive wound healing covers is an important task in medicine. The use of autologous platelet-rich plasma (PRP) consisting of growth factors, cytokines and components of extracellular matrix is a perspective approach for DFU treatment, but we previously found that some T2DM PRP samples have a toxic effect on mesenchymal stem cells (MSCs) in vitro. Here, we covalently immobilized T2DM PRP proteins on polycaprolactone (PCL) nanofibers, and the growth of endothelial cells on the PCL-COOH-PRP was investigated. Additionally, the level of NO reflecting the cytotoxic effects of PRP, angiogenin, and VEGF levels were measured in T2DM PRP samples. The results showed that the application of PCL-COOH-PRP nanofibers allows to remove the cytotoxicity of T2DM PRP and to improve endothelial cell adhesion and proliferative activity. We showed that the origin of T2DM PRP (the level of PRP toxicity or presence/absence of DFU) does not influence the efficiency of cell growth on PCL-COOH-PRP, and on the level of angiogenin, vascular epidermal growth factor (VEGF) in PRP itself.

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Section: Optimization Of Pcl Nanofibers Structure and Prp Immobilizationmentioning

confidence: 63%

PRP of T2DM Patient Immobilized on PCL Nanofibers Stimulate Endothelial Cells Proliferation

Solovieva

Sitnikova

Nimaev

et al. 2023

IJMS

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“…Due to the hydrophobic surface and, accordingly, the low value of free surface energy, effective attachment and proliferation of cells on the surface of polylactic acid and PLA/HA composite materials does not occur. This problem can be solved by exposing the surface of the composite to low-temperature plasma in a nitrogen flow [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Plasma Treatment of Composite Materials Based on Polylactic Acid and Hydroxyapatite

Khomutova,

Korzhova,

Bryuzgina

et al. 2024

Polymers

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The effect of surface modification by an arc discharge plasma in a nitrogen flow with treatment durations of 5 and 10 min on the physicochemical properties and biocompatibility of the surface of composites based on polylactic acid and hydroxyapatite (PLA/HA) with different mass ratios (80/20, 70/30, 60/40) has been investigated. The aim of this work was to show the correlation between the changes of the physicochemical characteristics (chemical compound, morphology, wettability) of the surface layer of the PLA/HA composites and the cell viability (macrophages) in the presence of the plasma-modified materials. The dependence of alterations of the functional properties (wettability, biocompatibility) on the change in the chemical composition under the plasma exposure has been established. The chemical composition was studied using X-ray photoelectron spectroscopy (XPS), the surface morphology was researched with scanning electron microscopy (SEM), and the wettability of the composite’s surface was analyzed by measuring the contact angle and surface energy calculation. In addition, the viability of macrophages was investigated when the macrophages from three donors interacted with a modified PLA/HA surface. It was found that the formation of the new functional groups, –C-N and N-C=O/C=O, improves the wettability of the surface of the composites and promotes the viability of macrophages in the presence of the composite materials. The fundamental principles for obtaining promising materials with the required properties for eliminating bone defects have been created.

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Carboxylic acid functionalized para-xylene based hypercrosslinked polymer as a novel and high performance adsorbent for heavy metal removal

Rostami,

Moradi,

Ashourzadeh Pordsari

et al. 2024

Arabian Journal of Chemistry

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Immobilization and Release of Platelet-Rich Plasma from Modified Nanofibers Studied by Advanced X-ray Photoelectron Spectroscopy Analyses

Cited by 6 publications

References 43 publications

PRP of T2DM Patient Immobilized on PCL Nanofibers Stimulate Endothelial Cells Proliferation

PRP of T2DM Patient Immobilized on PCL Nanofibers Stimulate Endothelial Cells Proliferation

Nitrogen Plasma Treatment of Composite Materials Based on Polylactic Acid and Hydroxyapatite

Carboxylic acid functionalized para-xylene based hypercrosslinked polymer as a novel and high performance adsorbent for heavy metal removal

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