Immobilization-free electrochemical DNA detection with anthraquinone-labeled pyrrolidinyl peptide nucleic acid probe

An Elegant Analysis of White Spot Syndrome Virus Using a Graphene Oxide/Methylene Blue based Electrochemical Immunosensor Platform

“…Indeed, development of pathogen selective electrochemical immunosensor is a challenging research area. Previously, a couple of DNA based bio-electrochemical sensors were developed for the WSSV detection1718. Zhang et al reported a thiol functional group modified ssDNA (probe) self-assembled monolayer micro-electromechanical system as a sensor electrode and ferricyanide as a transducer for hybridization detection of targeted ssDNA (WSSV)17.…”

“…Zhang et al reported a thiol functional group modified ssDNA (probe) self-assembled monolayer micro-electromechanical system as a sensor electrode and ferricyanide as a transducer for hybridization detection of targeted ssDNA (WSSV)17. Kongpeth et al reported a anthraquinone-labeled pyrrolidinyl peptide nucleic acid (AQ-PNA) probe based immobilization-free detection of WSSV-ssDNA18. Note that, in the above DNA biosensors, several complicated and time consuming off-line preparation procedures including PCR or Loop mediated isothermal amplification (LAMP) amplification procedures have been used.…”

Single nucleotide polymorphism genotyping using DNA sequencing on multiwalled carbon nanotubes monolayer by CNT-plasmon resonance

“…Deposition of two monolayers formed from stearic acid micelles with MWCNTs inside on the surface on Fe-or FeCe-containing LB-FeDTP-film results in anchoring (7) and black (8) curves] of ON W and heteroduplex [light-green (1) and green (4) curves] of ON SNP with DNA isolated from the blood of patient with coloteral cancer with allele SNP-mutation (at excitation at 473 and 532 nm, respectively); heteroduplex [blue curve (5)] and homoduplex [light-blue curve (6)] between SNP-mutant DNA from cancer tissue and ON W or ON SNP , respectively (at excitation at 532 nm); homoduplex [brown curve (9)] of ON W and heteroduplex [yellow curve (3)] of ON SNP with DNA from cancer tissue (excitation at 473 nm). Power was 0.6 mW for green laser and 0.24 mW for blue laser.…”

“…Label-free DNA-sensors do not demand DNA sequence labeling that allows to avoid the stearic hindrance effects and the configuration loss of probe DNA at its labeling. [6][7][8] Sensing can be performed by Raman spectroscopy or electrochemical impedance spectroscopy methods, at that surface plasmon resonance phenomenon is often utilized to enhance the method sensitivity. The nanosensors based on graphene and carbon nanotubes (CNTs) are considered as the most perspective ones due to their high electro-conductivity in comparison with ordinary three-dimensional metals.…”

pH‐Driven Precise Control of Hybridization Reaction Kinetics for Rapid DNA Assay

“…Traditional methods for rapid DNA hybridization assay usually involve increasing the salt concentration and raising the temperature . The stiff conditions bring about instability in the system, and reflect a narrow window for those settings loaded with various fragile sensors, in particular biomolecules, for example, enzymes, peptide tags, and self‐assembly motifs . Besides, few DNA hybridization acceleration strategies have been developed except for those using isotachophoresis on a chip, cellulose fiber‐based paper test, addition of organic solvents in the reaction, and DNA detection on few‐layer graphdiyne nanosheets, which were designed through the strategy of enhancing local probe DNA concentration.…”