Single nucleotide polymorphism genotyping using DNA sequencing on multiwalled carbon nanotubes monolayer by CNT-plasmon resonance

Grushevskaya, H. V.; Крылова, Н. Г.; Lipnevich, I. V.; Егорова, Валентина Петровна; Babenka, A. S.

doi:10.1142/s0217979218400337

Cited by 8 publications

(4 citation statements)

References 28 publications

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“…In addition, the D and G peaks of SP/SWNT-PCA/DNAzyme shifted 2 cm −1 and 1 cm −1 in the negative direction after DNAzyme modification. The above results proved that EDC-NHS and DNAzyme had been modified with SWNTs-PCA [ 35 , 36 ].…”

Section: Resultsmentioning

confidence: 71%

Superhydrophobic Paper-Based Microfluidic Field-Effect Transistor Biosensor Functionalized with Semiconducting Single-Walled Carbon Nanotube and DNAzyme for Hypocalcemia Diagnosis

Wang

Chen

Zhang

et al. 2022

IJMS

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Hypocalcemia is caused by a sharp decline in blood calcium concentration after dairy cow calving, which can lead to various diseases or even death. It is necessary to develop an inexpensive, easy-to-operate, reliable sensor to diagnose hypocalcemia. The cellulose-paper-based microfluidic field-effect biosensor is promising for point-of-care, but it has poor mechanical strength and a short service life after exposure to an aqueous solution. Octadecyltrichlorosilane (OTS), as a popular organosilane derivative, can improve the hydrophobicity of cellulose paper to overcome the shortage of cellulose paper. In this work, OTS was used to produce the superhydrophobic cellulose paper that enhances the mechanical strength and short service life of MFB, and a microfluidic field-effect biosensor (MFB) with semiconducting single-walled carbon nanotubes (SWNTs) and DNAzyme was then developed for the Ca2+ determination. Pyrene carboxylic acid (PCA) attached to SWNTs through a non-covalent π-π stacking interaction provided a carboxyl group that can bond with an amino group of DNAzyme. Two DNAzymes with different sensitivities were designed by changing the sequence length and cleavage site, which were functionalized with SPFET/SWNTs-PCA to form Dual-MFB, decreasing the interference of impurities in cow blood. After optimizing the detecting parameters, Dual-MFB could determine the Ca2+ concentration in the range of 25 μM to 5 mM, with a detection limit of 10.7 μM. The proposed Dual-MFB was applied to measure Ca2+ concentration in cow blood, which provided a new method to diagnose hypocalcemia after dairy cow calving.

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Section: Resultsmentioning

confidence: 71%

Superhydrophobic Paper-Based Microfluidic Field-Effect Transistor Biosensor Functionalized with Semiconducting Single-Walled Carbon Nanotube and DNAzyme for Hypocalcemia Diagnosis

Wang

Chen

Zhang

et al. 2022

IJMS

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“…The FWCNTs were decorated by the nanocyclic organometallic LBcomplexes. The FWCNT LB-arrays decorated by the dithienylpyrrole complexes of octahedral high spin Fe(II) host spin-polarized graphene charge carriers [31][32][33].…”

Section: Fabrication Of Dithienylpyrrole-coated Fwcnt Arraysmentioning

confidence: 99%

High sensitivity electrochemical DNA sensors for detection of somatic mutations in FFPE samples

Egorova¹,

Grushevskaya²,

Крылова³

et al. 2022

Preprint

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We offer new high-performance label-free electrochemical impedimetric DNA sensors of nonfaradaic type. The DNA sensors based on a platform of crystalline carbon nanotube (CNT) arrays are fabricated by the Langmuir-Blodgett (LB) deposition technique. The CNT arrays are suspended on a nanoporous anodic alumina (aluminium oxide, AOA) support. Single-stranded (ss) 19-and 20-base oligonucleotides and double-stranded (ds) nucleotide sequences were used as probes for chosen molecular target -human KRAS (Kirsten Rat Sarcoma viral oncogene homolog) gene. Genomic deoxyribonucleic acids (DNAs) were isolated from placenta of healthy donors and FFPE (formalin-fixed, paraffinembedded) samples of tumor tissue. Raman spectral analysis and electrochemical dielectric spectroscopy were used to sequence the target DNAs. The optical and electrochemical detection (variants of DNA sequencing) were based on a screening effect that grows after the DNA homoduplex formation. We demonstrated that such technologies are very sensitive and allow to detect attomolar DNA concentrations and less. As result, the KRAS exon 2, codon 12, c.35G>A mutation was successfully discriminated in human genomic DNA isolated from FFPE colorectal cancer tumor tissue samples.

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“…Because of these facts new methods of DNAanalysis should be developed [1,2]. The most promising method for sequencing of single DNA molecules in samples is nanosequencing based on surface plasmon resonance effects and surface-enhanced Raman scattering in quantum materials: nanoparticles, graphene, carbon nanotubes, graphene-like monomolecular layers (monolayers) and nanostructured composite materials [3,4]. In the present work we utilize long ds-and short ss-DNA-probes for label-free genotyping.…”

Section: Introductionmentioning

confidence: 99%

“…(a) Raman spectra of decorated MWCNTs after hybridization of target DNA T with KRAS w (spectra 1, 3) and KRAS m (spectra 2, 4) on MWCNT surface. Wavelengths of laser excitation were 532 (1, 2), 785 nm(3,4); (b) Dielectric spectra: "1" for nanoporous AOA, "2" for metal-containing DTP-LB-film deposited on AOA, "3" for decorated MWCNTs suspended on nanopores; (c) dielectric spectra: "1" and "2" for DNA-sensor with KRAS w and a probe system KRAS w -ds-DNA-probe respectively, "3" after bindin with target ss-DNA lm . Cole-Cole plots in the spectra are marked as Ai, i = 0, …, 5 for AOA; "P" for the DTP compound and Ci, i = 0, …, 3 for MWCNTs; Si, i = 0, …, 3 for DNA-sensor with KRAS w only; "'On"' for ds-DNA probe; "DNA" for duplex.…”

mentioning

confidence: 99%

A Single-Molecule Label-Free Identification of Single-Nucleotide Colorectal-Cancer-DNA Polymorphism Using Impedance Spectroscopy of Self-Redox-Active Decorated Carbon Nanotubes

et al. 2020

Self Cite

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A novel label-free impedance assay which is based on self-redox active carbon nanotube arrays decorated by nanocyclic organometallic complex monolayer is offered to discriminate single-nucleotide polymorphism of colorectal-tumor genome. The assay is based on the following effects. A mass transfer for the multiwalled carbon nanotubes (MWCNTs) is testified by a Cole-Cole plot with Warburg impedance in dielectric spectra. Charging K(K')-point of graphene Brillouin zone is associated with the mass transfer for MWCNTs that leads to arising of charged MWCNT-end states. Plasma oscillations of charge carriers shield electric field of charged electrodes. Complementary hybridization of target deoxyribonucleic acid (DNA) with probe DNA on the MWCNT surface facilitates the penetration of single-stranded target DNA in the nanopores. Dielectric-band appearance and shielding effects testify the duplex formation.

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Single nucleotide polymorphism genotyping using DNA sequencing on multiwalled carbon nanotubes monolayer by CNT-plasmon resonance

Cited by 8 publications

References 28 publications

Superhydrophobic Paper-Based Microfluidic Field-Effect Transistor Biosensor Functionalized with Semiconducting Single-Walled Carbon Nanotube and DNAzyme for Hypocalcemia Diagnosis

Superhydrophobic Paper-Based Microfluidic Field-Effect Transistor Biosensor Functionalized with Semiconducting Single-Walled Carbon Nanotube and DNAzyme for Hypocalcemia Diagnosis

High sensitivity electrochemical DNA sensors for detection of somatic mutations in FFPE samples

A Single-Molecule Label-Free Identification of Single-Nucleotide Colorectal-Cancer-DNA Polymorphism Using Impedance Spectroscopy of Self-Redox-Active Decorated Carbon Nanotubes

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