Immobilization-free screening of aptamers assisted by graphene oxide

Park, Jee-Woong; Tatavarty, Rameshwar; Kim, Dae Woo; Jung, Hee Tae; Gu, Man Bock

doi:10.1039/c2cc16473f

Cited by 159 publications

(105 citation statements)

References 27 publications

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“…The aptamers were screened with rGO. First, the ssDNA library was bound to rGO via π‐π stacking interactions between the polycyclic aromatic rings of the graphene surface and the DNA bases . Next, nonbound ssDNAs on rGO were separated and removed.…”

Section: Resultsmentioning

confidence: 99%

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Development of a ssDNA aptamer system with reduced graphene oxide (rGO) to detect nonylphenol ethoxylate in domestic detergent

Kim

Jung

et al. 2018

J of Molecular Recognition

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Endocrine‐disrupting chemicals are a major public health problem throughout the world. In the human body, these compounds functionalize the same as sexual hormones, inducing precocious puberty, gynecomastia, etc. To help prevent this occurrence, a simple detection system is needed. In this study, a nonylphenol ethoxylate (NPE)‐specific aptamer was selected by reduced graphene oxide‐systematic evolution of ligands by exponential enrichment. A random ssDNA library was incubated with rGO for adsorption, followed by elution with the target molecule. As a result of screening, a DNA aptamer was found that specifically bounds to the target with high binding affinity (Kd = 100.9 ± 13.2 nM) and had a low limit of detection (LOD = 696 pM). Furthermore, this NPE‐binding aptamer bounds selectively to the target. Characterization of the aptamer was confirmed by measuring the fluorescence signal recovery from rGO. In addition, detection of NPE was performed with several water samples, and the detection accuracy was 100 ± 10%. From these results, we expect that this aptamer could be applied to an on‐site detection system for NPE in industrial sites or domestic fields.

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Section: Resultsmentioning

confidence: 99%

“…However, some cases of target binding are very complex and time‐consuming processes. To overcome this problem, we processed SELEX using reduced graphene oxide (rGO) for screening small molecules . Generally, graphene oxide (GO) can bind single‐stranded oligonucleotides via π‐π interactions .…”

Section: Introductionmentioning

confidence: 99%

Development of a ssDNA aptamer system with reduced graphene oxide (rGO) to detect nonylphenol ethoxylate in domestic detergent

Kim

Jung

et al. 2018

J of Molecular Recognition

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“…Previously, SPR-based aptasensor for proteins are well established and widely used to detect various proteins such as tubulin (Fukusaki et al, 2001), thrombin and thyroid transcription factor 1 (Murphy et al, 2003), HIV-1 Tat protein (Tombelli et al, 2005), C-reactive protein (Bini et al, 2008), human immunoglobulin E (IgE) (Kim et al, 2009a) and adipokines Park et al, 2012), by a single-site binding configuration. Concerning to the signal amplification issue, the two-site binding configuration was adopted that combined the aptamers and antibodies binding to a specific target.…”

Section: Surface Plasmon Resonance (Spr)-based Aptasensorsmentioning

confidence: 99%

Aptamer-based nanobiosensors

Kim¹,

Raston

2016

Biosensors and Bioelectronics

366

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“…Importantly, this bioanalytical strategy can be enhanced by designing probes with a significant difference in their adsorption energy via GO. [60] Moreover, this biosensing mechanism has been extended to aptamer selection, [61] evanescent wave aptasensors, [62] peptide-based biosensing platforms, [63] biomolecular logic gates operations, [64,65] in situ live cell sensing, [66][67][68] and drug monitoring. [60] Moreover, this biosensing mechanism has been extended to aptamer selection, [61] evanescent wave aptasensors, [62] peptide-based biosensing platforms, [63] biomolecular logic gates operations, [64,65] in situ live cell sensing, [66][67][68] and drug monitoring.…”

Section: Innovative Trends Of Go In Optical Biosensingmentioning

confidence: 99%

Graphene Oxide as an Optical Biosensing Platform: A Progress Report

2018

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IntroductionAs a 2D material, graphene oxide (GO) is a lattice-like nanostructure of hexagonal carbon rings disrupted by oxygen-containing moieties. In 2012, we discussed the outstanding physicochemical properties offered by GO and how these features can be exploited in unprecedented optical biosensing systems. [1] In fact, GO can be processed in suspension, easily complexed with biomolecules, and offers a universal highly efficient long-range photoluminescence quenching agent-among other functional properties. Furthermore, we highlighted that GO photoluminescences with energy transfer donor/acceptor molecules exposed A few years ago, crucial graphene oxide (GO) features such as the carbon/oxygen ratio, number of layers, and lateral size were scarcely investigated and, thus, their impact on the overall optical biosensing performance was almost unknown. Nowadays valuable insights about these features are well documented in the literature, whereas others remain controversial. Moreover, most of the biosensing systems based on GO were amenable to operating as colloidal suspensions. Currently, the literature reports conceptually new approaches obviating the need of GO colloidal suspensions, enabling the integration of GO onto a solid phase and leading to their application in new biosensing devices. Furthermore, most GO-based biosensing devices exploit photoluminescent signals. However, further progress is also achieved in powerful label-free optical techniques exploiting GO in biosensing, particularly using optical fibers, surface plasmon resonance, and surface enhanced Raman scattering. Herein, a critical overview on these topics is offered, highlighting the key role of the physicochemical properties of GO. New challenges and opportunities in this exciting field are also highlighted.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adma.201805043.in a planar surface. [1] However, GO properties can be tailored according to its oxidation degree, lateral size, and number of layers, which was something little explored 6 years ago, and thus their impact on the overall biosensing performance was almost unknown. In addition, most of the biosensing systems based on GO were amenable to working as colloidal suspensions. Though, recent literature reports conceptually new approaches obviating the need of colloidal suspensions, which facilitates integration of GO-based biosensors into the solid phase and allows for their applications in new devices. Furthermore, the majority of the GO-based optical biosensing techniques rely on photoluminescent signals. However, further progress has also been achieved in powerful label-free optical techniques exploiting GO in biosensing. Here, we introduce a progress report on this exciting topic, underscoring challenges and opportunities in (bio)sensing accordingly. Number of LayersGO aqueous suspensions are highly stable in the form of mono layers. However, GO multilayer films can be built via

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Immobilization-free screening of aptamers assisted by graphene oxide

Cited by 159 publications

References 27 publications

Development of a ssDNA aptamer system with reduced graphene oxide (rGO) to detect nonylphenol ethoxylate in domestic detergent

Development of a ssDNA aptamer system with reduced graphene oxide (rGO) to detect nonylphenol ethoxylate in domestic detergent

Aptamer-based nanobiosensors

Graphene Oxide as an Optical Biosensing Platform: A Progress Report

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