Immobilization of Trametes versicolor laccase on different PGMA‐based polymeric microspheres using response surface methodology: Optimization of conditions

Abstract

Enzymatic immobilization is a versatile alternative to improve enzyme stability and enable its reuse. In this study, the change in the immobilized enzyme properties due to the type of functional group on the carrier was evaluated. For that, monodisperse polymeric microspheres with two functional groups widely used in laccase immobilization—oxirane [poly(glycidyl methacrylate) (PGMA)] and hydrazide—were synthesized by dispersion polymerization to covalently immobilize the laccase Trametes versicolor. Using a response surface methodology, laccase immobilization was optimized for each microsphere type. As a result, laccase immobilization on PGMA carriers appears to broaden the pH and temperature ranges, storage stability, and reusability compared to free and hydrazide enzymes. These aforementioned characteristics indicate that PGMA microspheres could act as an ideal support for enzyme immobilization in biotechnological applications. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45249.

Polymer supports for the removal and degradation of hazardous organic pollutants: an overview

“…The majority of immobilizations by covalent binding in the support occur due to the reaction of the functional groups with lysine groups, generally on the enzyme surface. These are highly reactive, available and usually do not affect the active center of the enzyme . In this sense, both the physical properties (morphology, porosity, particle size etc.)…”

“…and chemical characteristics (various types of functional groups, spacer groups and amino acid residues, among others) . In recent years, the most commonly used supports are those that have amino, epoxide, glutaraldehyde and carbonyl groups . These supports have been synthesized according to the applications of the processes, but they can also be commercially available as are Eupergit C or Sepabeads…”

“…These are highly reactive, available and usually do not affect the active center of the enzyme. 24 In this sense, both the physical properties (morphology, porosity, particle size etc.) and chemical properties (functional groups, density of reactive groups, active functionality etc.)…”

“…Fortunately, many of these limitations can be overcome using various immobilization strategies on polymer supports. This provides the possibility of reuse, easier product separation, extra stability towards denaturing conditions and prolonged stability in operational conditions . In this context, a large variety of polymers have been used as support matrices, generating novel preparations with excellent mechanical, thermal and biochemical properties, suitable for bioremediation uses .…”

“…This provides the possibility of reuse, easier product separation, extra stability towards denaturing conditions and prolonged stability in operational conditions. 24,25 In this context, a large variety of polymers have been used as support matrices, generating novel preparations with excellent mechanical, thermal and biochemical properties, suitable for bioremediation uses. [26][27][28] The versatility of polymers is thus key to obtaining the desired carrier as well as providing different properties to the immobilized enzyme according to the immobilization method used and the kind of polymer-enzyme interaction selected.…”