Immobilization-induced hypersensitivity associated with spinal cord sensitization during cast immobilization and after cast removal in rats

Hamaue, Yohei; Nakano, Jun; Sekino, Yuki; Chuganji, Sayaka; Sakamoto, J.; Yoshimura, Tsuyoshi; Origuchi, Tomoki; Okita, Minoru

doi:10.1007/s12576-013-0277-4

Cited by 31 publications

(34 citation statements)

References 34 publications

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“…We previously investigated the underlying mechanisms of immobilization‐induced pain using a rat model. Two weeks of cast immobilization induced superficial pain in the skin, which was exacerbated when the immobilization period was prolonged . The mechanisms underlying the immobilization‐induced pain are likely associated with upregulation of nerve growth factor (NGF) and increased density of peripheral nerve fibers in the skin …”

Section: Introductionmentioning

confidence: 99%

Mechanisms underlying immobilization‐induced muscle pain in rats

Oga

Goto²,

Sakamoto

et al. 2020

Muscle and Nerve

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Introduction:We investigated the mechanisms underlying immobilization-induced muscle pain in rats. Methods:In rat skeletal muscle, pressure pain threshold (PPT) of the gastrocnemius muscle was measured, and nerve growth factor (NGF) level, peripheral nerve fiber density, macrophage number, and interleukin-1β (IL-1β) mRNA expression were examined. An NGF receptor inhibitor was injected intramuscularly to assess the relationship between PPT and NGF levels.Results: Immobilization resulted in a decrease in PPT and increases in NGF level, C-fiber density, M1 macrophage number, and IL-1β mRNA expression. Injection of NGF receptor inhibitor reversed the decrease in PPT.Discussion: NGF upregulation may be a major contributor to immobilization-induced muscle pain. The increases in C-fiber density, M1 macrophage number, and IL-1β mRNA expression may be related to immobilization-induced muscle pain. K E Y W O R D SC fiber, immobilization, M1 macrophage, muscle pain, NGF

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Section: Introductionmentioning

confidence: 99%

Mechanisms underlying immobilization‐induced muscle pain in rats

Oga

Goto²,

Sakamoto

et al. 2020

Muscle and Nerve

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“…However, after 42 days injection, secondary hyperalgesia in the CPM group was confirmed, which was mild compared to that of the IM group. Previous study demonstrated that eight-week joint immobilization induced hyperalgesia to mechanical stimulation associated with central sensitization in the spinal cord (Hamaue et al 2013). This may influence the decrease in the pain threshold in the CPM group.…”

Section: Discussionmentioning

confidence: 94%

“…Vol. 65 studies have suggested that immobilization may cause hyperalgesia with central sensitization in the spinal cord (Terkelsen et al 2008, Hamaue et al 2013. Terkelsen et al (2008) observed pain induced by transient movement and mechanical hypersensitivity in the distal forearm of human subjects after 4 weeks of cast immobilization.…”

Section: Introductionmentioning

confidence: 99%

“…Terkelsen et al (2008) observed pain induced by transient movement and mechanical hypersensitivity in the distal forearm of human subjects after 4 weeks of cast immobilization. Hamaue et al (2013) demonstrated that cast immobilization produces a time-dependent increase in mechanical hyperalgesia and that calcitonin gene-related peptide (CGRP) expression in the deeper lamina layer of the spinal dorsal horn increases in rats that have been immobilized for 8 weeks. These findings suggest that it is necessary to keep the affected limb as active as possible during the acute phase of inflammation.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Continuous Passive Motion Initiated After the Onset of Arthritis on Inflammation and Secondary Hyperalgesia in Rats

Nakabayashi

Sakamoto

Kataoka³

et al. 2016

Physiol Res

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This study investigated the effect of continuous passive motion (CPM) initiated after the onset of arthritis in rats. Rats were injected with 3 % kaolin/carrageenan in the knee joint and randomized to the control, immobilization (IM), or CPM group. The knee joints of the IM and CPM groups were immobilized with a cast for 56 days. In the CPM group, CPM exercise was administered for 60 min/day (6 times/week). Joint transverse diameter and pressure pain threshold (PPT) were assessed as indicators of inflammation, and paw withdrawal response (PWR) was assessed as indicator of secondary hyperalgesia. Central sensitization was analyzed by measuring calcitonin gene-related peptide (CGRP) expression levels in the spinal dorsal horn. In the CPM group, the PPT was significantly increased compared with the IM group from 14 to 35 days, and PWR was significantly decreased from 14 to 56 days. Additionally, CGRP expression in the super facial layer (I-II) of the spinal dorsal horn (L4-5) in the CPM group was significantly decreased compared with the IM group. Our study found the CPM initiated after the onset of arthritis promoted the recovery of inflammation and mitigated secondary hyperalgesia

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“…However, a prolonged increase of pain facilitation, impairment of sympathetic reflexes, and prolonged limb immobilization which may have short‐term benefits, has many long‐term negative consequences, including spreading neuroinflammation and developing chronic CRPS symptoms. Both, presynaptic adrenergic stimulation of sensory afferents (D1) and immobilization (V2) stimulate peripheral neuroinflammation , and the lack of inhibitory control mechanism (L2) may produce and maintain a vicious circle, in which a facilitated neuroinflammation maintains afferent nociceptive input, which in turn maintains D1‐L2‐V2 response pattern.…”

Section: Involvement Of Peripheral and Central Nervous Systemsmentioning

confidence: 99%