Immobilization of a fibronectin fragment at the surface of a polyetherurethane film

Breuers, W.; Klee, Doris; Höcker, Hartwig; Mittermayer, Ch.

doi:10.1007/bf00703468

Cited by 22 publications

(15 citation statements)

References 10 publications

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“…140 Klee and co-workers plasma-polymerized vinyl acetate, 141 of which the grafted polymer was subsequently hydrolyzed with aqueous sodium methoxide to give hydroxyl groups at the surface of a poly(etherurethane). 142 Plasma polymerization can also be performed to polymerize molecules which do not present any polymerizable group. 143 Under the action of the plasma, the polymeric surface becomes activated -most probably radicals are created -and the monomer in its gas state is fragmented into different species and undergoes polymerization on the surface to yield a highly uniform film.…”

Section: Post-processing Introduction Of Chemical Anchors Onto Polyme...mentioning

confidence: 99%

Chemical approaches to synthetic polymer surface biofunctionalization for targeted cell adhesion using small binding motifs

et al. 2012

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Section: Post-processing Introduction Of Chemical Anchors Onto Polyme...mentioning

confidence: 99%

Chemical approaches to synthetic polymer surface biofunctionalization for targeted cell adhesion using small binding motifs

et al. 2012

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“…Par exemple, des fonctions hydroxyles sont créées en surface de poly(vinyl acetate) via une hydrolyse [39] en milieu alcalin. Une aminolyse du poly(c-methyl L-glutamate) par des diamines telles que l'hydrazine hydratée, l'éthylènedia-mine ou l'hexaméthylendiamine amènent à la formation de surfaces fonctionnalisées par des fonctions amines qui peuvent être utilisées dans le cadre d'immobilisation de peptides [40].…”

Section: Fonctionnalisation De Matériaux Par Des Peptides Rgdunclassified

Conception, élaboration et caractérisation de matériaux bioactifs

Durrieu

2005

ITBM-RBM

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“…Most synthetic polymers do not have appropriate functional groups, which can conjugate biological molecules covalently on their surfaces 22. Blending,28–32 copolymerization,33–39 interpenetrating networking,40, 41 chemical,42–44 and physical45–47 treatments have been extensively investigated to introduce charged functional groups onto polymeric scaffold surfaces. However, synthetic polymers are not adequate for the application as bone grafting materials because they have no osteoconductivity at all.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of biodegradable poly(ε‐caprolactone)‐organosiloxane hybrid with carboxylate groups

2012

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Biodegradable poly(ε-caprolactone)-organosiloxane hybrid with carboxylate groups was newly synthesized by a sol-gel method with the capacity to conjugate cell adhesion ligands for the potential applications as a bone tissue engineering scaffold material. An intermediate hybrid contained positively charged amine groups was synthesized by end-capping α,ω-hydroxyl poly(ε-caprolactone) with (3-isocyanatopropyl)triethoxysilane and a subsequent sol-gel reaction with (3-aminopropyl)triethoxysilane. This resulted in a new hybrid composed of biodegradable organic poly(ε-caprolactone) segments connected by inorganic siloxane linkages containing positively charged amine groups. The successive succinlylation of amine groups created amide bonds and converted the cationic sites of succinic anhydride into negatively charged carboxylate groups. New formations of amine and carboxylate groups could be directly and indirectly confirmed using Fourier transformed infrared spectrometry, zeta potentials, and atomic force microscopy. Biodegradability of hybrid with carboxylate groups was also examined by weight loss in phosphate buffered saline, and it was about 14 wt % after 8 weeks. Conjugating capacity of a cell adhesion ligand was examined by comparing the relative fluorescent intensities of covalently and noncovalently immobilized synthetic GGGGRGDASSK-FITC oligopeptides onto the carboxylated groups of the hybrid using confocal laser scanning microscopy; the relative fluorescent intensity of covalently conjugated RGD peptide was about three times higher than that of noncovalently coated RGD peptide. The hybrid covalently conjugated by GGGGRGDASSK oligopeptides improved proliferation and differentiation activities of preosteoblastic MC3T3-E1 cells. The results suggest that this hybrid possesses an encouraging potential to be used as a bone tissue engineering scaffold material due to its biodegradability and high capacity for conjugating cell adhesion ligands.

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Immobilization of a fibronectin fragment at the surface of a polyetherurethane film

Cited by 22 publications

References 10 publications

Chemical approaches to synthetic polymer surface biofunctionalization for targeted cell adhesion using small binding motifs

Chemical approaches to synthetic polymer surface biofunctionalization for targeted cell adhesion using small binding motifs

Conception, élaboration et caractérisation de matériaux bioactifs

Synthesis of biodegradable poly(ε‐caprolactone)‐organosiloxane hybrid with carboxylate groups

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