Immobilization of alliinase on porous aluminum oxide

Milka, Peter; Krest, Ingo; Keusgen, Michael

doi:10.1002/1097-0290(20000805)69:3<344::aid-bit13>3.0.co;2-d

Cited by 36 publications

(27 citation statements)

References 13 publications

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“…Candida antarctica lipase B Hydrolysis of p-nitrophenyl palmitate Alumina [9] Rat hepatic microsomal CYP2E1 Para-nitrophenol hydroxylation Alumina [15] Alliinase Conversion of alliin in alliicin Alumina [16] Glucose-6-phosphate dehydrogenase Oxidation of Glucose-6 phosphate (G6P) Alumina [17] Silica [18] Laccase Oxidation of 2,2 1 -azino-bis-(3 ethylbenzothiazoline-6-sulfonic acid) Titania [19] Urease Urea hydroxylation Alumina [20] Glutathione transferase Glutathione conjugation to 1-chloro-2,4-dinitrobenzene Alumina [21] One of the most studied biocatalysts is lipase, which is active in different reactions such as hydrolysis, esterification, and transesterification, with high enantioselective properties [22]. Lipase is an interfacial molecule with phase transfer catalytic properties, meaning it performs to the best of its abilities in an oil/water interface [23].…”

Section: Immobilized Proteinmentioning

confidence: 99%

Use of a Ceramic Membrane to Improve the Performance of Two-Separate-Phase Biocatalytic Membrane Reactor

Ranieri

Mazzei

et al. 2016

Molecules

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Biocatalytic membrane reactors (BMR) combining reaction and separation within the same unit have many advantages over conventional reactor designs. Ceramic membranes are an attractive alternative to polymeric membranes in membrane biotechnology due to their high chemical, thermal and mechanical resistance. Another important use is their potential application in a biphasic membrane system, where support solvent resistance is highly needed. In this work, the preparation of asymmetric ceramic hollow fibre membranes and their use in a two-separate-phase biocatalytic membrane reactor will be described. The asymmetric ceramic hollow fibre membranes were prepared using a combined phase inversion and sintering technique. The prepared fibres were then used as support for lipase covalent immobilization in order to develop a two-separate-phase biocatalytic membrane reactor. A functionalization method was proposed in order to increase the density of the reactive hydroxyl groups on the surface of ceramic membranes, which were then amino-activated and treated with a crosslinker. The performance and the stability of the immobilized lipase were investigated as a function of the amount of the immobilized biocatalytst. Results showed that it is possible to immobilize lipase on a ceramic membrane without altering its catalytic performance (initial residual specific activity 93%), which remains constant after 6 reaction cycles.

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Section: Immobilized Proteinmentioning

confidence: 99%

Use of a Ceramic Membrane to Improve the Performance of Two-Separate-Phase Biocatalytic Membrane Reactor

Ranieri

Mazzei

et al. 2016

Molecules

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“…In this study, the biotin-coated gold electrodes used with a layer of APTES (Davidson et al, 2004;Phadtare et al, 2004;Yakovleva et al, 2003), thus creating an amino surface with biotin-NHS match ends formed. On this layer and biotinylated streptavidin antibody (Milka et al, 2000) on the application of CRP measurement was carried out by applying the secondary antibody. K dis , antigen-antibody method, the values can be determined easily.…”

Section: Immunosensors Based Spr For Detection Of Crpmentioning

confidence: 99%

Biosensors for Cancer Biomarkers

Uygun¹,

Sezgintürk²

2011

Biosensors - Emerging Materials and Applications

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“…A cystein-sulfoxide biosensor based on the enzyme alliinase and an ammonia gas electrode has been developed (Fig 12). It is based on the conversion of cystein sulfoxides such as alliine into sulfenic and pyruvic acids and ammonia [115,116]. The sensor had a detection limit of 0.5 mM alliine and could be operated over 400 h. It was used for the screening of Allium species from various origins for the presence of cysteine sulfoxides.…”

Section: Biosensorsmentioning

confidence: 99%

Natural Products in Drug Discovery - Concepts and Approaches for Tracking Bioactivity

Potterat¹,

Hamburger

2006

COC

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Effective methods for localization and characterization of bioactivity are a cornerstone for natural product based drug discovery efforts. Over the last decade, a wealth of new technologies and conceptual approaches for bioactivity screening have emerged. These developments are reviewed under the perspective of their applicability in the field of natural products discovery. The methods discussed here include bioautography, HPLC-based activity profiling, HPLC-based on-flow bioassays, assays based on capillary electrophoresis, molecular imprinted polymers, various MSand NMR-based methods, biosensors, and chip-based technologies for affinity separation and expression profiling. Selected examples illustrate the potential and limitations of the different approaches for contemporary natural products lead discovery.

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Immobilization of alliinase on porous aluminum oxide

Cited by 36 publications

References 13 publications

Use of a Ceramic Membrane to Improve the Performance of Two-Separate-Phase Biocatalytic Membrane Reactor

Use of a Ceramic Membrane to Improve the Performance of Two-Separate-Phase Biocatalytic Membrane Reactor

Biosensors for Cancer Biomarkers

Natural Products in Drug Discovery - Concepts and Approaches for Tracking Bioactivity

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