Immobilization of Biomolecules on Langmuir−Blodgett Films of Regenerative Cellulose Derivatives

Abstract

We present a new and fast method for preparation of ultrathin layers of biomolecules on solid substrates and its application as a useful tool for sensing biomolecules in an affinity biosensor. Nonamphiphilic trimethylsilyl ether-cellulose monolayers are used as interfaces to reduce nonspecific interactions between solid surfaces and biomolecules. These films are transferred on optical waveguides by the Langmuir-Blodgett technique. After their stabilization by cleavage of the hydrophobic trimehylsilyl groups, the cellulose films serve as excellent matrixes for the immobilization of proteins at high density. Cyanurchloride serves as a cross-linker between the cellulose layer and the proteins. The activity and biological specificity of these interfaces is controlled by an enzymatic assay and direct sensing by an evanescent wave immunosensor.

Adsorption of phosphoric acid on niobium oxide coated cellulose fiber: preparation, characterization and ion exchange property

“…About 100 mg of the adsorbent were immersed in 25 mL of metal chloride solutions, whose concentrations varied between 10 -3 at 10 -4 mol L -1 , and the resulting suspensions were orbitally shaken for 6 h. The metal ions in the supernatant solutions were separated by decanting from the solid phase and their concentrations determined by inductive coupled plasma (ICP) optical emission spectroscopy (OES) on a PerkinElmer 3000 DV ICP optical emission spectrometer. The quantities of the exchanged metals were determined by applying equation (1).…”

“…8%) for the materials. Figure 3a shows the O 1s (BE) peak at 532.9 eV associated with pure cellulose, designated as O 1 . Upon modification of the surface by coating with metal oxide (Figure 3b 26 The pure oxide shows the Nb 3d 5/2 BE at 207.4 eV.…”

“…The reactions are carried under very mild conditions. The hybrid material obtained, Cel/ Nb/phosphate, was characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, and solid state 31 ) and the suspensions shaken for 12 h at 298 K. The quantity of phosphoric acid adsorbed on Cel/Nb 2 O 5 along the isotherm curve was determined by applying the equation: (1) where N a is the initial mole number of phosphoric acid added into the reaction flask, N s is the equilibrium mole number of the phosphoric acid in the solution phase in equilibrium with the solid phase and m is the mass of the adsorbent. The experiment was carried out by the batch procedure and the analysis of the phosphate ion in the solution phase was made by the spectrophotometric method.…”

Trends in polymer chemistry 1998

“…With many systems however, bonding of the macromolecules occurred via a highly ordered layer of low-molecular compounds [53,61,62]. This way it succeeded to couple enzymes, antibodies and proteins to planar solid-state surfaces for the building-up of biosensors [61]. Tao et al used the known micro-printing technique to apply laterally defined structures of monomolecular thiol layers to gold.…”

Biotin‐Functionalized Cellulose‐Based Monolayers as Sensitive Interfaces for the Detection of Single Molecules

“…[26][27][28][29] Highly ordered, stable and homogeneous films can be obtained by the use of novel cellulose derivatives, the so-called "hairy-rod" polymers, as basic material. [30,31] The high stability is achieved by the moderately ordered, partially entangled cellulose molecules, which have a preferred orientation along the dipping direction after being transferred from a Langmuir film balance onto solid substrates.…”