IMMOBILIZATION OF CARBONIC ANHYDRASE ENZYME PURIFIED FROM<i>Bacillus subtilis</i>VSG-4 AND ITS APPLICATION AS CO<sub>2</sub>SEQUESTERER

Oviya, M.; Giri, Sib Sankar; Sukumaran, V.; Natarajan, P

doi:10.1080/10826068.2012.654571

Cited by 31 publications

(10 citation statements)

References 23 publications

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“…Wu, Liu, Zhou, Wang, Xia and Liu () isolated three active secondary metabolites from Alcaligenes faecalis FY‐3 that induce immune‐related gene expression in C. idellus kidney cells. Oviya, Giri, Sukumaran and Natarajan () and Giri, Sen, Jun, Sukumaran and Park () demonstrated that B. subtilis VSG4, isolated from tropical soil, produces carbonic anhydrase, and the secondary metabolite (a biosurfactant) isolated from this strain (200 μg mL ‒1 ) positively influences immune responses, enhances disease resistance and stimulates immune‐related gene expression in L. rohita . The results of Giri et al .…”

Section: Microorganismsmentioning

confidence: 98%

Application of immunostimulants in aquaculture: current knowledge and future perspectives

et al. 2016

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Similar to other industries, aquaculture constantly requires new techniques to increase production yields. Modern technologies and different scientific fields, such as biotechnology and microbiology, provide important tools that could lead to a higher quality and a greater quantity of products. New feeding practices in farming typically play an important role in aquaculture, and the addition of various additives to a balanced feed formula to achieve better growth is a common practice of many fish and shrimp feed manufacturers and farmers. As 'biofriendly agents', immunostimulants, such as biological factors, probiotics and vitamins, can be introduced into the culture environment to control and kill pathogenic bacteria, as well as to promote growth of the cultured organisms. In addition, immunostimulants are non-pathogenic and nontoxic and do not produce undesirable side effects when administered to aquatic organisms. In this review, we summarize previous studies performed with both traditional immunostimulants and the most promising new generation of immunostimulants, such as polysaccharides, nutrients, oligosaccharides, herbs, microorganisms, prebiotics and different biological factors. This review primarily focuses on their protective efficacies and on what is known concerning their effects on the immune systems of aquatic organisms when delivered in vivo.

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Section: Microorganismsmentioning

confidence: 98%

Application of immunostimulants in aquaculture: current knowledge and future perspectives

et al. 2016

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“…The immobilized forms are efficient and can be used for industrial purposes. The CA isolated from B. subtilis VSG4 convert CO 2 into CaCO 3 in the immobilized form (Oviya et al, 2012). Bacillus sp.…”

Section: Improvement Of Soil Qualitymentioning

confidence: 99%

Bacillus subtilisimpact on plant growth, soil health and environment: Dr. Jekyll and Mr. Hyde

Mahapatra

Yadav

Ramakrishna

2022

Journal of Applied Microbiology

140

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The increased dependence of farmers on chemical fertilizers poses a risk to soil fertility and ecosystem stability. Plant growth‐promoting rhizobacteria (PGPR) are at the forefront of sustainable agriculture, providing multiple benefits for the enhancement of crop production and soil health. Bacillus subtilis is a common PGPR in soil that plays a key role in conferring biotic and abiotic stress tolerance to plants by induced systemic resistance (ISR), biofilm formation and lipopeptide production. As a part of bioremediating technologies, Bacillus spp. can purify metal contaminated soil. It acts as a potent denitrifying agent in agroecosystems while improving the carbon sequestration process when applied in a regulated concentration. Although it harbours several antibiotic resistance genes (ARGs), it can reduce the horizontal transfer of ARGs during manure composting by modifying the genetic makeup of existing microbiota. In some instances, it affects the beneficial microbes of the rhizosphere. External inoculation of B. subtilis has both positive and negative impacts on the endophytic and semi‐synthetic microbial community. Soil texture, type, pH and bacterial concentration play a crucial role in the regulation of all these processes. Soil amendments and microbial consortia of Bacillus produced by microbial engineering could be used to lessen the negative effect on soil microbial diversity. The complex plant–microbe interactions could be decoded using transcriptomics, proteomics, metabolomics and epigenomics strategies which would be beneficial for both crop productivity and the well‐being of soil microbiota. Bacillus subtilis has more positive attributes similar to the character of Dr. Jekyll and some negative attributes on plant growth, soil health and the environment akin to the character of Mr. Hyde.

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“…We previously showed that Bacillus subtilis VSG4, isolated from tropical soil (Oviya et al, 2012), produces biosurfactant that stimulates immune responses and cytokine gene expression in rohu ( Labeo rohita ), in addition to enhancing resistance to A. hydrophila infection in carp (Giri et al, 2016c). In the present study, a biosurfactant-producing strain, Bacillus licheniformis , was isolated from food waste.…”

Section: Introductionmentioning

confidence: 99%

Role of Bacillus licheniformis VS16-Derived Biosurfactant in Mediating Immune Responses in Carp Rohu and its Application to the Food Industry

et al. 2017

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Multifarious applications of Bacillus licheniformis VS16-derived biosurfactant were explored. Labeo rohita fingerlings were injected intraperitoneally with 0.1 mL of phosphate-buffered saline (PBS) containing purified biosurfactant at 0 (control), 55 (S55), 110 (S110), 220 (S220), or 330 (S330) μg mL-1 concentrations. Various immunological parameters and the expression of immune-related genes were measured at 7, 14, and 21 days post-administration (dpa). At 21 dpa, fish were challenged with Aeromonas hydrophila and mortality was recorded for 14 days. Immune parameters such as lysozyme levels (39.29 ± 2.14 U mL-1), alternative complement pathway (61.21 ± 2.38 U mL-1), and phagocytic activities (33.37 ± 1.2%) were maximum (P < 0.05) in the S220 group at 14 dpa; but immunoglobulin levels (11.07 ± 0.83 mg mL-1) were highest in the S220 group at 7 dpa, compared to that in controls. Activities of digestive enzymes (amylase, protease, and lipase) were higher (P < 0.05) in the S220 and S330 groups than in the control group. Regarding cytokine gene expression, pro-inflammatory cytokines (TNF-α and IL-1β) were down-regulated (P < 0.05) in the S220 and S330 groups. Expression of IL-10, TGF-β, and IKB-α were up-regulated in the S220 and S330 groups at 14 dpa, with the highest levels in the S220 group. The expression of NF-κB p65 and IKK-β were down-regulated in treatment groups, and were lowest (P < 0.05) in the S220 group. The highest post-challenge survival rate (72.7%) was recorded in S220 group. Further, the potential of this substance to inhibit biofilm formation, and heavy metal removal from vegetables were also evaluated. Biosurfactant was effective in inhibiting biofilm formation up to 54.71 ± 1.27%. Moreover, it efficiently removed cadmium (Cd) from tested vegetables such as carrot, radish, ginger, and potato, with the highest removal efficiency (60.98 ± 1.29%) recorded in ginger contaminated with Cd. Collectively, these results suggest that isolated biosurfactant could be used in the aquaculture industry, in addition to its potential application to the food industry.

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IMMOBILIZATION OF CARBONIC ANHYDRASE ENZYME PURIFIED FROMBacillus subtilisVSG-4 AND ITS APPLICATION AS CO₂SEQUESTERER

Cited by 31 publications

References 23 publications

Application of immunostimulants in aquaculture: current knowledge and future perspectives

Application of immunostimulants in aquaculture: current knowledge and future perspectives

Bacillus subtilisimpact on plant growth, soil health and environment: Dr. Jekyll and Mr. Hyde

Role of Bacillus licheniformis VS16-Derived Biosurfactant in Mediating Immune Responses in Carp Rohu and its Application to the Food Industry

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IMMOBILIZATION OF CARBONIC ANHYDRASE ENZYME PURIFIED FROMBacillus subtilisVSG-4 AND ITS APPLICATION AS CO2SEQUESTERER

Cited by 31 publications

References 23 publications

Application of immunostimulants in aquaculture: current knowledge and future perspectives

Application of immunostimulants in aquaculture: current knowledge and future perspectives

Bacillus subtilisimpact on plant growth, soil health and environment: Dr. Jekyll and Mr. Hyde

Role of Bacillus licheniformis VS16-Derived Biosurfactant in Mediating Immune Responses in Carp Rohu and its Application to the Food Industry

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Product

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IMMOBILIZATION OF CARBONIC ANHYDRASE ENZYME PURIFIED FROMBacillus subtilisVSG-4 AND ITS APPLICATION AS CO₂SEQUESTERER