Immobilization of chondroitin sulfate to lipid membranes and its interactions with ECM proteins

Altgärde, Noomi; Becher, Jana; Möller, Stephanie; Weber, Franz E.; Schnabelrauch, Matthias; Svedhem, Sofia

doi:10.1016/j.jcis.2012.07.063

Cited by 14 publications

(7 citation statements)

References 49 publications

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“…Understanding GAG-related interactions is inherently difficult and requires proper and defined platforms. Svedhem et al showed two immobilization strategies to chemically couple the GAG chondroitin sulfate (CS) to fluid SLBs: 1. by activating carboxyl groups on CS prior to the addition of an amino-functionalized lipid, i.e., 1,2-dioleoylsn-glycero-3-phosphoethanolamine-N-(lauroylamine) (DOPE-NH2); 2. by activating carboxy-functionalized phospholipids, such as 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lauroyl) (DOPE-COOH), followed by the addition of hydrazide-functionalized CS (Altgarde et al, 2013). The formation of SLBs and subsequent conjugation was followed in situ using QCM-D.…”

Section: Extracellular Matrix-supported Lipid Bilayer Systemsmentioning

confidence: 99%

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

Huang

2019

Front. Mater.

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The cell membrane is an organized and fluid structure that modulates cellular activities in response to specific extracellular signals, and maintains the critical communication, integration, and homeostasis between the cytosol and the extracellular matrix (ECM). In recent years, tissue engineering and cell biology research has been rapidly progressed by a remarkable understanding of cell and ECM interfaces. In this review, the design of new biomimetic platforms based on the conjugation of ECM proteins on solid supported lipid bilayers (SLBs) will be summarized. The platforms provide a better system to evaluate cellular responses to specific recognition events, gradient, mechanical property, nanostructures, and inter-and intra-molecular interactions of ECM proteins on a non-fouling and fluid membrane. Moreover, the findings from the molecular interactions and cellular activities will be highlighted to look into the cell-materials mechanisms.

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Section: Extracellular Matrix-supported Lipid Bilayer Systemsmentioning

confidence: 99%

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

Huang

2019

Front. Mater.

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“…[16][17][18][19] Thus, it is important to monitor the concentrations of thesei ons and to understand more deeply thef actorst hat govern their accumulation in the ECM of specific tissues and by specific organisms. [24][25][26] These shortcomings suggestt hat well-defined saccharides are required to study the exact effect of sulfation on metalb inding properties. [22,23] The effect of sulfation of saccharides on heavy metal binding is largely unknown because it is very difficultt oo btain saccharides with defined size and sulfation pattern.…”

Section: Introductionmentioning

confidence: 99%

“…[2,3] Alternatively, artificial sulfated polymers such as polystyrene sulfonate have been used as replacements for saccharides but these compounds lack the rich structural properties of the native entities. [24][25][26] These shortcomings suggestt hat well-defined saccharides are required to study the exact effect of sulfation on metalb inding properties. [27] Sulfated hyaluronic acids (sHA) are synthetic oligosaccharides derived from hyaluronica cid (HA) that largely resemble natives ulfated GAG such as chondroitin,d ermatan or heparan sulfate ( Figure 1A and C).…”

Section: Introductionmentioning

confidence: 99%

“…The effect of sulfation of saccharides on heavy metal binding is largely unknown because it is very difficult to obtain saccharides with defined size and sulfation pattern . Alternatively, artificial sulfated polymers such as polystyrene sulfonate have been used as replacements for saccharides but these compounds lack the rich structural properties of the native entities . These shortcomings suggest that well‐defined saccharides are required to study the exact effect of sulfation on metal binding properties …”

Section: Introductionmentioning

confidence: 99%

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Sulfation Patterns of Saccharides and Heavy Metal Ion Binding

Alshanski

Blaszkiewicz

Mervinetsky

et al. 2019

Chemistry A European J

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Sulfated saccharides are an essentialp art of extracellularm atrices, and they are involvedi nalarge number of interactions. Sulfated saccharide matricesi no rganismsa ccumulate heavy metal ions in addition to othere ssentialm etal ions. Accumulation of heavy metal ions alters the function of the organisms and cells, resulting in severea nd irreversible damage. The effect of the sulfation pattern of saccharides on heavym etal binding preferences is enigmatic because the accessibility to structurally definedsulfated sac-charides is limited and because standard analytical techniquescannot be used to quantify these interactions.Wedeveloped an ew strategy that combines enzymatic and chemicals ynthesis with surfacec hemistry and label-free electrochemical sensing to study the interactions between well-defined sulfated saccharides and heavy metal ions. By using these tools we showedt hat the sulfation pattern of hyaluronic acid governs their heavy metal ions binding preferences.

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“…7,8 The complexity of biological membranes makes direct studies very difficult; thus, simple model systems that can mimic the cellular environment, such as micelles and lipid vesicles, have been used. [9][10][11] Lipid vesicles are concentric uni-or multilamellar lipid bilayers that have layer diameters Vol. 27, No.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Xanthene Dyes in DPPC Vesicles: Rationally Accounting for Drug Partitioning Using a Membrane Model

Calori¹,

Pellosi²,

Vanzin³

et al. 2016

Journal of the Brazilian Chemical Society

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The correct selection of a dye that has effective action as a photosensitizer is a primary concern for successful therapeutic outcomes. The effectiveness of the photodynamic agent is related to both the targeting of cell membranes and the photochemical yield of the chosen dye. The distributions of xanthene derivatives Eosin Y, Erythrosin B, and Rose Bengal B in vesicles of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in both liquid-crystalline and gel phases were investigated by fluorescence spectroscopy. Binding constants, fluorescence anisotropy, fluorescence quenching, fluorescence quantum yield, and fluorescence resonance energy transfer at physiological pH conditions were determined. To Erythrosin B and Eosin Y, the iodide quenching rate constant was shown to involve a sphere of action mechanism driven by a specific interaction between Erythrosin B and Eosin Y molecules and the choline head-group of the phospholipid; in contrast, Rose Bengal B was located deep in the membrane and this mechanism was not present. The dyes can be ordered by their penetration depth in the membrane, and this order was found to be Eosin Y < Erythrosin B < Rose Bengal B. These results demonstrate a rational approach for the screening of more active agents for photodynamic therapy based on the affinity between the xanthene derivatives and DPPC vesicles.

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Immobilization of chondroitin sulfate to lipid membranes and its interactions with ECM proteins

Cited by 14 publications

References 49 publications

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

Sulfation Patterns of Saccharides and Heavy Metal Ion Binding

Distribution of Xanthene Dyes in DPPC Vesicles: Rationally Accounting for Drug Partitioning Using a Membrane Model

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