Immobilization of DNA on CMOS compatible materials

Organic Surface Modification of Silicon Nanowire-Based Sensor Devices

“…Alternative approaches have been reported as well, e.g., the use of UV/ozone for the surface activation, followed by the use of neat APTES (Li, 2010). Also toluene (Lobert, 2003) and acetone (Lin, 2007) have been used as a solvent in the silanization step.…”

Sensitive DNA electrical detection based on interdigitated Al/Al2O3 microelectrodes

“…After standard cleaning, 400 nm wet thermal silicon oxide was grown at 1000 • C in order to obtain high densities of DNA acceptor endings, OH groups, at the surface [13]. The interdigitated Al fingers were realized on top of the oxide by lift-off process involving positive photo-resist deposition, optical mask photo-definition, etch of illuminated resist, 500 nm thick aluminum evaporated and removal of remaining resist and Al residues.…”

“…The biochips were functionalized with the presence of aldehyde in order to fix aminated oligonucleotides, based on the method of Lobert et al [13]. In short, the biochips were incubated with a 10/90 water/methanol solution, containing 7-octenyltrimethoxysilane 3 mM, and the terminal alcene groups of the linkers were transformed in aldehyde groups by oxidation with 1.5 mM KMnO 4 and 60 mM NaIO 4 .…”

Label-free, direct DNA detection by means of a standard CMOS electronic chip

“…However, gold is not a standard material in the commercial realization processes of solid state chips. Lobert et al [2] demonstrated the possibility of functionalizing CMOS-and bio-compatible materials, like silica and native alumina, with a proper coating of the surfaces with silane species; in particular, silica resulted more suitable for functionalization than native alumina, which is poorly uniform and robust to chemical treatments.…”