Immobilization of enzymes in liquid membranes for enantioselective hydrolysis

Meyer, Elazar; Scheper, Thomas; Hitzmann, Bernd; Schügerl, K.

doi:10.1007/bf01876163

Cited by 12 publications

(4 citation statements)

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“…The extraction of phenols from blood and plasma is accomplished by liquid surfactant membrane technique 23 . Liquid membrane have been widely used in biotechnological application [24][25][26][27][28][29][30] . Biological implications of liquid membrane hypothesis have also been investigated recently [31][32] .…”

Section: Liquid Membranes In Biological Applicationsmentioning

confidence: 99%

On the Biological Applications of Liquid Membranes : A Short Review

S¹

2023

IJFMR

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Studies on permeation through membranes have attracted attention during the last few decades because of the various applications of membranes in our day today life. Studies on Bulk liquid membranes (BLM). Emulsion liquid membranes (ELM) Supported liquid membranes (SLM) all have led to wonderful applications in technology and biology. An attempt is being made to throw light on the applications these liquid membranes in various biological fields viz facilitated transfer controlled release of drugs and all pharmaceutical applications.

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Section: Liquid Membranes In Biological Applicationsmentioning

confidence: 99%

On the Biological Applications of Liquid Membranes : A Short Review

S¹

2023

IJFMR

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“…Liquid membrane emulsions made of paraffin were used to encapsulate wheat germ lipase [56]. This technique allowed the combination of enzymatic hydrolysis of rac-4-acetoxy-cyclopentenone with a selective transport through the organic phase constituting the membrane.…”

Section: Immobilization Of Wheat Germ Lipasementioning

confidence: 99%

Wheat germ lipase: isolation, purification and applications

Kublicki

Koszelewski

Brodzka

et al. 2021

Critical Reviews in Biotechnology

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In recent years, wheat germ lipase (WGL) is attracting considerable interest. To date, several WGL applications have already been described: (i) fats and oils modification; (ii) esterification reactions in organic media, accepting a wide range of acids and alcohols as substrates; (iii) the asymmetric resolution of various chiral racemic intermediates; (iv) more recently, the promiscuous activity of WGL has been shown in carbon-carbon bond formation. To date, no crystallographic structure of this enzyme has been published, which means its activity, catalytic potential and substrate scope is being assessed empirically. Therefore, new catalytic activities of this enzyme are constantly being discovered. Taking into account the emergency and the current interest in environmentally sustainable processes, this review aims to highlight the origin, isolation, stabilization by immobilization and applications of the wheat germ lipase. HIGHLIGHTSWheat germ as an inexpensive source of biocatalysts Wheat germ lipase an efficient catalyst for various chemical transformations Wheat germ lipase in food production Industrial applications of wheat germ lipase Wheat germ lipase as a promiscuous biocatalyst Immobilization of wheat germ lipase as a method of stabilization ARTICLE HISTORY

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“…The insolubilized enzymes have the practical advantage of being easily recovered at the end of the reaction, a property of considerable importance for their industrial application. Although numerous methods for enzyme immobilization processes have been widely explored, microencapsulation of enzymes within liquid membranes which are permeable to substrate and products but not to the enzymes has attracted much interest by different research groups (May and Li, 1972;Li, 1974, 1975;Scheper et al, 1984Scheper et al, , 1987Makryaleas et al, 1985;Meyer et al, 1988;Chang and Lee, 1993) only in the recent past. Immobilization of the enzyme on a solid matrix suffers several drawbacks.…”

Section: Introductionmentioning

confidence: 99%

Mathematical Analysis of Unsteady-State Dynamics of a Liquid-Membrane-Encapsulated Urease System

Chowdhury

Bhattacharya

1997

Ind. Eng. Chem. Res.

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In the present investigation, a mathematical model describing the transient behavior of a water in oil type liquid-membrane-immobilized enzyme system has been presented. A deterministic model (as opposed to a stochastic one) has been presented in an attempt to elucidate the effect of mass-transfer limitation due to immobilization on the kinetics of hydrolysis of urea using an unstructured Michaelis-Menten equation and a mechanistic approach toward diffusion phenomena. An immobilized urease system has been selected to test the model within the initial substrate (urea) concentration range 0.001-4 M. With the help of concentration distribution curves of the product-carrier complex as a function of dimensionless length along each globule of emulsion with time as a parameter, an attempt has been made to show how mass-transfer resistance affects the reaction engineering behavior of the system. It has been observed that the product distribution and reaction velocity as a function of substrate concentration as predicted from the proposed model explains reality quite satisfactorily.

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Immobilization of enzymes in liquid membranes for enantioselective hydrolysis

Cited by 12 publications

References 4 publications

On the Biological Applications of Liquid Membranes : A Short Review

On the Biological Applications of Liquid Membranes : A Short Review

Wheat germ lipase: isolation, purification and applications

Mathematical Analysis of Unsteady-State Dynamics of a Liquid-Membrane-Encapsulated Urease System

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