Immobilization of enzymes in photochemically cross-linked polyvinyl alcohol

Cotton gauze bandage: a support for protease immobilization for use in biomedical applications

“…This phenomenon is usually observed when enzymes are covalently coupled to supports (Kennedy, Cabral, 1987). Similar results for immobilized trypsin and other proteases have also been reported, as for example, in the immobilization of trypsin on soap-free P(MMA-EA-AA) latex particles (Kang et al, 2005) and acrylic copolymers (Bryjak, Kolarz, 1998), in the immobilization of an extracellular proteinase from Thermus strain Rt41A in pore glass beads (Wilson et al, 1994) and in the immobilization of amyloglucosidase and invertase in polyvinyl alcohol (Uhlich et al, 1996).…”

Immobilization of catalase in poly(isopropylacrylamide-co-hydroxyethylmethacrylate) thermally reversible hydrogels

“…In addition, it has also been reported that hydrogel carriers such as Sephadex, Sepharose, poly(2-hydroxyethyl methacrylate) and poly(acrylamide) protect enzymes from thermal inactivation and yield higher thermal stabilities. 20,26 On the basis of these observations, pNIPAM/HEMA 2 is a hydrophilic network which induces higher thermal stability compared to that of its free counterpart.…”

Development of Hydrogel‐Based Keratoprostheses: A Materials Perspective

“…PVA is an excellent choice of material because it has been extensively reported to be both biocompatible and resistant to protein adsorption and adhesion. It has been used in a number of biomedical applications including contact lenses, ophthalmic implants (66), tendon repair (67), cartilage repair (68,69), and drug delivery (70,71). PVA hydrogels are most commonly formed by crystallization induced by freeze-thaw methods, chemical crosslinking with aldehydes, or radiation (32,72).…”