Immobilization of enzymes on heterofunctional epoxy supports

Mateo, César; Grazú, Valeria; Palomo, José M.; López‐Gallego, Fernando; Fernández‐Lafuente, Roberto; Guisán, José M.

doi:10.1038/nprot.2007.133

Cited by 279 publications

(195 citation statements)

References 52 publications

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“…The most interesting immobilization procedures are in the area of covalent binding. Supports containing epoxy groups are widely used in the immobilization by covalent binding because these generate intense multipoint covalent attachment with different nucleophiles present on the surface of the enzyme molecules (Mateo et al, 2007). One limitation of the epoxy supports is the slow reaction of immobilization.…”

Section: Immobilization Of Enzymesmentioning

confidence: 99%

See 1 more Smart Citation

Hydrolysis of Lignocellulosic Biomass: Current Status of Processes and Technologies and Future Perspectives

Verardi¹,

De²,

Ricca³

et al. 2012

Bioethanol

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Section: Immobilization Of Enzymesmentioning

confidence: 99%

“…Cys, Hys, and Tyr). Epoxy groups also react, in a slower way, with carboxylic groups (Mateo et al, 2007). The support used in this immobilization method is typically a prefabricated carrier, such as synthetic resins, biopolymers, inorganic polymers such as silica or zeolites.…”

Section: Immobilization Of Enzymesmentioning

confidence: 99%

Hydrolysis of Lignocellulosic Biomass: Current Status of Processes and Technologies and Future Perspectives

Verardi¹,

De²,

Ricca³

et al. 2012

Bioethanol

View full text Add to dashboard Cite

“…It is known that multipoint covalent attachment can stabilize the immobilized enzymes [31,32]. Moreover, it was demonstrated that multipoint attachment could enhance the operational stability of immobilized enzymes in microfluidic reactor as well [33].…”

Section: Covalent Immobilization Of Pcpal Onto Swcnt Nh2mentioning

confidence: 99%

Aminated Single-walled Carbon Nanotubes as Carrier for Covalent Immobilization of Phenylalanine Ammonia-lyase

Bartha-Vári

Bencze

Bell

et al. 2017

Period. Polytech. Chem. Eng.

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“…6,55,56 To prime the gels for attachment, 2 mm diameter 4 wt. % agarose gel discs were isolated on Teflon AF-coated glass slides.…”

Section: Enzyme Microreactor Assaymentioning

confidence: 99%

Hydrogel discs for digital microfluidics

Fiddes

Luk

et al. 2012

Biomicrofluidics

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Hydrogels are networks of hydrophilic polymer chains that are swollen with water, and they are useful for a wide range of applications because they provide stable niches for immobilizing proteins and cells. We report here the marriage of hydrogels with digital microfluidic devices. Until recently, digital microfluidics, a fluid handling technique in which discrete droplets are manipulated electromechanically on the surface of an array of electrodes, has been used only for homogeneous systems involving liquid reagents. Here, we demonstrate for the first time that the cylindrical hydrogel discs can be incorporated into digital microfluidic systems and that these discs can be systematically addressed by droplets of reagents. Droplet movement is observed to be unimpeded by interaction with the gel discs, and gel discs remain stationary when droplets pass through them. Analyte transport into gel discs is observed to be identical to diffusion in cases in which droplets are incubated with gels passively, but transport is enhanced when droplets are continually actuated through the gels. The system is useful for generating integrated enzymatic microreactors and for three-dimensional cell culture. This paper demonstrates a new combination of techniques for lab-on-a-chip systems which we propose will be useful for a wide range of applications.

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Immobilization of enzymes on heterofunctional epoxy supports

Cited by 279 publications

References 52 publications

Hydrolysis of Lignocellulosic Biomass: Current Status of Processes and Technologies and Future Perspectives

Hydrolysis of Lignocellulosic Biomass: Current Status of Processes and Technologies and Future Perspectives

Aminated Single-walled Carbon Nanotubes as Carrier for Covalent Immobilization of Phenylalanine Ammonia-lyase

Hydrogel discs for digital microfluidics

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