Immobilization of enzymes on nanoinorganic support materials: An update

Jamalidoust, Marzieh; Hemmati, Roohullah; Homaei, Ahmad; Dinari, Ali; Jamlidoost, Marzieh; Tashakor, Amin

doi:10.1016/j.ijbiomac.2020.11.127

Cited by 151 publications

(78 citation statements)

References 142 publications

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“…Immobilization methods and support materials are directly related to final enzyme orientation and kinetic parameters. 46 In contrast, the half-life (t 1/2 ) of enzymes can be improved by immobilization (Table 1), as the method increases enzyme stability. Immobilization minimizes or prevents the exposure of enzymes to inactivation agents/conditions, providing a favorable enzyme environment, enhancing the stability of enzyme conformations, increasing enzyme rigidification via multipoint covalent attachment and preventing subunit dissociation in multimeric enzymes.…”

Section: Free Versus Immobilized Enzymesmentioning

confidence: 99%

Enzyme immobilization: what have we learned in the past five years?

Almeida

Prata

Forte

2021

Biofuels Bioprod Bioref

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Immobilized enzymes have been widely applied in several fields over the past years, and the number of studies on the topic has been rapidly increasing. This review aimed to examine changes and trends in enzyme immobilization research during the past five years. Data were collected from the Web of Science Core Collection database and analyzed using VOSviewer © software. A discussion is presented on the most productive countries, most cited authors, leading publications, main areas of research and industrial applications of immobilized enzymes. China, the USA, India, Brazil and Spain hold the largest numbers of published articles on the topic, and the two main areas of study are chemistry and biotechnology applied microbiology. Trend analysis showed that laccases and lipases were the most commonly used enzymes, whereas nanoparticles and metal-organic frameworks were the most commonly used enzyme supports. The results highlight that immobilization is used to modify several enzyme properties, such as stability, recovery and specificity. Conclusions and future perspectives in the field are also addressed. It is believed that the development of new, low-cost supports and studies on the scale-up of processes using immobilized enzymes constitute future research trends.

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Section: Free Versus Immobilized Enzymesmentioning

confidence: 99%

Enzyme immobilization: what have we learned in the past five years?

Almeida

Prata

Forte

2021

Biofuels Bioprod Bioref

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“…To do not require this step, the use of immobilized enzymes, which are enzymes bound to an inert and insoluble material, can be used. Immobilized enzymes can be recovered from the extract and reused, which can make the process cheaper (Ashkan et al, 2021).…”

Section: Recovery Of Phenoli C Comp Ounds From G R Ape Re S Idue By Enz Ymati C Tre Atmentmentioning

confidence: 99%

New trends in the use of enzymes for the recovery of polyphenols in grape byproducts

et al. 2021

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Residues from wine and juice processing still contain about 70% of the phenolic compounds in grapes. These compounds are valued for having several bioactive properties that are explored in the pharmaceutical and food sectors. This paper aims to summarize the most recent advances in the use of enzymatic techniques for the recovery of bioactive compounds from GP for industrial application. For this, we analyzed scientific articles and patent applications from the last 20 years in the main indexed and patent databases. Among the most used enzymes in the recovery of bioactive compounds in wastes, cellulases, pectinases, tannases, glucoamylases, and proteases such as trypsin and chymotrypsin, are the most important. As a result, extracts are obtained with greater retrieval of compounds such as anthocyanins, gallic acid, catechins, epicatechins, and trans-resveratrol and the improvement of coloring, anti-inflammatory, antioxidant and vasoprotective properties. Although the use of enzymes for the recovery of phenolics is an old strategy, the number of studies focusing on the functional characteristics and industrial applicability of the extracts obtained has been recently growing. Practical applicationsPhenolic compounds have acted as anti-inflammatories, antioxidants, anticarcinogens, and antimicrobials, being additives or relevant ingredients for various products in the food and pharmaceutical industry. Although there are several techniques for extracting/recovering phenolics from grape pomace, there is still no agreement on which method is ideal. In recent years, several extractions methods have been applied in seeking optimized conditions to recover phenolics from grape residues. Among them, the use of enzymes has been gaining attention for being considered a green and promising technology. The present study aims to carry out a review that would bring a new perspective to the recovery of bioactive compounds from grape residues by enzymatic techniques, with a view to industrial purpose.

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“…Natural and synthetic polymers were applied as insoluble materials for the immobilization of the enzymes [ 18 ]. Hyaluronic acid (HA), the main constituent of the extracellular matrix (ECM) in NP tissue [ 19 ], has various functional groups that enable the strong enzyme-matrix interactions.…”

Section: Introductionmentioning

confidence: 99%