Immobilization of ?-fructofuranosidase from Aspergillus japonicus on chitosan using tris(hydroxymethyl)phosphine or glutaraldehyde as a coupling agent

Cheng, Tzu-Chien; Duan, Kow‐Jen; Sheu, Dey-Chyi

doi:10.1007/s10529-005-0984-x

Cited by 29 publications

(16 citation statements)

References 7 publications

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“…A previous study showed the use of THP as a coupling agent retained 20% more residual activity than the use of glutaraldehyde 9. Therefore, in this work, we immobilized β‐galactosidase on to chitosan using THP as a coupling agent to produce GOS from lactose.…”

Section: Introductionmentioning

confidence: 99%

Application of tris(hydroxymethyl)phosphine as a coupling agent for β‐galactosidase immobilized on chitosan to produce galactooligosaccharides

Cheng

Duan

Sheu

2005

J of Chemical Tech & Biotech

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β-Galactosidase was immobilized on chitosan using tris(hydroxymethyl)phosphine (THP) as a coupling agent to produce galactooligosaccharides (GOS) from lactose. Both the THP-immobilized and the free enzymes were maximally achieved at pH 5.0 and the optimal temperature was 55• C. The residual activities for the THP-immobilized enzyme and the free enzyme were 75 and 25%, respectively, after being incubated in 0.1 mol dm −3 sodium acetate buffer (pH 5.0) at 55 • C for 13 days. The formation of GOS was catalyzed by free and THP-immobilized β-galactosidase from lactose. The yield of GOS produced by the free enzyme from the lactose solution (36%, w/v) at 55• C was 43% on a dry weight basis, which was similar to the 41% GOS yield produced by the THP-immobilized enzyme system.

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Section: Introductionmentioning

confidence: 99%

Application of tris(hydroxymethyl)phosphine as a coupling agent for β‐galactosidase immobilized on chitosan to produce galactooligosaccharides

Cheng

Duan

Sheu

2005

J of Chemical Tech & Biotech

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“…Fructofuranosidases with fructosyltransferase activity can be used to produce fructooligosaccharides (FOS), as 1-kestose and 1-nystose, prebiotic substances that have important functional properties (Toledo et al 2004;Maiorano et al 2008). Microorganisms have been used to produce these enzymes, especially filamentous fungi such as Aspergillus niger (Rubio and Navarro, 2006), Aspergillus japonicus (Cheng et al 2005), Aspergillus ochraceus (Guimarães et al 2007), Aspergillus niveus (Guimarães et al 2009) and Cladosporium cladosporioides (Almeida et al 2005), among others. The production of β-Dfructofuranosidase by fungi is conducted under Submerged Fermentation (SbmF) or under Solid State Fermentation (SSF) (Romero-Gómez et al 2000;Aranda et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Biochemical properties of an extracellular β-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate

Rustiguel¹,

Oliveira²,

Terenzi³

et al. 2011

Electro Journal of Biotech

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The filamentous fungus A. phoenicis produced high levels of β-D-fructofuranosidase (FFase) when grown for 72 hrs under Solid-State Fermentation (SSF), using soy bran moistened with tap water (1:0.5 w/v) as substrate/carbon source. Two isoforms (I and II) were obtained, and FFase II was purified 18-fold to apparent homogeneity with 14% recovery. The native molecular mass of the glycoprotein (12% of carbohydrate content) was 158.5 kDa with two subunits of 85 kDa estimated by SDS-PAGE. Optima of temperature and pH were 55ºC and 4.5. The enzyme was stable for more than 1 hr at 50ºC and was also stable in a pH range from 7.0 to 8.0. FFase II retained 80% of activity after storage at 4ºC by 200 hrs. Dichroism analysis showed the presence of random and β-sheet structure. A. phoenicis

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“…Chang et al [15] have made chitosan-ZrO 2 composite beads by adding ZrO 2 powders to chitosan/acetic acid slurry. Cheng et al [16] have used tris(hydroxymethyl) phosphine (THP) instead of glutaraldehyde (GA) as a coupling agent to prepare THP-activated chitosan beads. Chiou et al have developed two agents to treat chitosan: one is carbodiimide used for activating the hydroxyl groups of chitosan [17], and the other is genipin, a natural cross-linker of plant origin, used for chitosan cross-linking [13].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Aspergillus niger Xylanase on Chitosan Using Dialdehyde Starch as a Coupling Agent

Chen

Liu

et al. 2009

Appl Biochem Biotechnol

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Dialdehyde starch (DAS) was used as a novel coupling agent to prepare chitosan carrier to immobilize the xylanase from Aspergillus niger A-25. Compared with glutaraldehyde-cross-linked chitosan (CS-GA) and pure chitosan beads, the DAS-cross-linked chitosan (CS-DAS) beads exhibited the highest xylanase activity recovery. The DAS adding amount and cross-linking time in CS-DAS preparation process were optimized with respect to activity recovery to the values of 1.0 g (6.7% w/v concentration) and 16 h, respectively. The optimum temperature of both the CS-DAS- and CS-GA-immobilized xylanase was observed to be 5 degrees C higher than that of free enzyme (50 degrees C). The CS-DAS-immobilized xylanase had the highest thermal and storage stability as compared to the CS-GA-immobilized and free xylanase. The apparent K (m) and V (max) values of the CS-DAS-immobilized xylanase were estimated to be 1.29 mg/ml and 300.7 mumol/min/mg protein, respectively. The CS-DAS-immobilized xylanase could produce from birchwood xylan high-quality xylo-oligosaccharides, mainly composed of xylotriose, as free xylanase did. The proposed CS-DAS carrier was more advantageous over the CS-GA or pure chitosan carrier for xylanase immobilization application.

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Immobilization of ?-fructofuranosidase from Aspergillus japonicus on chitosan using tris(hydroxymethyl)phosphine or glutaraldehyde as a coupling agent

Cited by 29 publications

References 7 publications

Application of tris(hydroxymethyl)phosphine as a coupling agent for β‐galactosidase immobilized on chitosan to produce galactooligosaccharides

Application of tris(hydroxymethyl)phosphine as a coupling agent for β‐galactosidase immobilized on chitosan to produce galactooligosaccharides

Biochemical properties of an extracellular β-D-fructofuranosidase II produced by Aspergillus phoenicis under Solid-Sate Fermentation using soy bran as substrate

Immobilization of Aspergillus niger Xylanase on Chitosan Using Dialdehyde Starch as a Coupling Agent

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