Immobilization of glucose oxidase on chitosan-based porous composite membranes and their potential use in biosensors

Susanto, Heru; Samsudin, Asep Muhamad; Rokhati, Nur; Widiasa, I Nyoman

doi:10.1016/j.enzmictec.2013.02.005

Cited by 62 publications

(30 citation statements)

References 28 publications

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“…It was reported that disruption of the DIT1 gene required for the synthesis of dityrosine layer leads to the loss of this layer but an intact chitosan layer exposed to the outside [16]. Chitosan, a natural polyaminosaccharide, is recommended as an ideal support material for enzyme immobilization, especially for food, pharmaceutical, and medical applications due to its excellent properties, such as non-toxicity, biocompatibility, and biodegradability [1,27,31]. 1 3 d-Psicose, the C-3 epimer of d-fructose, exhibits special properties of being an ideal sucrose substitute.…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of d-glucose to d-psicose with immobilized d-xylose isomerase and d-psicose 3-epimerase on Saccharomyces cerevisiae spores

Duan

et al. 2015

Journal of Industrial Microbiology and Biotechnology

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Saccharomyces cerevisiae spores are dormant cells, which can tolerate various types of environmental stress. In our previous work, we successfully developed biological and chemical methods for enzyme immobilization based on the structures of S. cerevisiae spore wall. In this study, we employed biological and chemical approaches for the immobilization of D-xylose isomerase (XI) from Thermus thermophilus and D-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens with yeast spores, respectively. The enzymatic properties of both immobilized XI and DPEase were characterized and the immobilized enzymes exhibit higher thermostability, broader pH tolerance, and good repeatability compared with free enzymes. Furthermore, we established a two-step approach for the bioconversion of D-glucose to D-psicose using immobilized enzymes. To improve the conversion yield, a multi-pot strategy was adopted for D-psicose production by repeating the two-step process continually. As a result, the yield of D-psicose was obviously improved and the highest yield reached about 12.0 %.

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Section: Introductionmentioning

confidence: 99%

Bioconversion of d-glucose to d-psicose with immobilized d-xylose isomerase and d-psicose 3-epimerase on Saccharomyces cerevisiae spores

Duan

et al. 2015

Journal of Industrial Microbiology and Biotechnology

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“…This band probably imine group that usually appears in ~1661 cm -1 . This imine group appeared as a result of the reaction between the amine group in chitosan and the aldehyde group on the glutaraldehyde (Susanto et al, 2013). The IR spectra also shows the increasing of corresponding groups in ~1993 cm -1 that indicate ether/acetal (-COC) group.…”

Section: Frequency Fiber Diameter (Nm)mentioning

confidence: 88%

Impact of post-treatment on the characteristics of electrospun poly (vinyl alcohol)/chitosan nanofibers

Susanto¹,

Samsudin²,

Faz³

et al. 2016

AIP Conference Proceedings

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Abstract. Electrospun nanofibers have many advantages such as high porosity, easy to be fabricated in various size and high ratio of surface area to volume. This paper presents the preparation of electrospun PVA/Chitosan nanofibers and more specifically focuses on the effect of post-treatment on the permeability and morphology of electrospun PVA/chitosan nanofibers. The mixtures of various concentrations of PVA (6,7,8 wt%)and 2 wt%.chitosan solution (with the ratio of 3:1)were used in electrospun with a constant rate of 0.7 ml/hour. The post-treatment was conducted by immersing in a ethanol or glutaraldehyde solution to performed crosslink structure. The electrospun PVA/Chitosan nanofiber was characterized by scanning electron microscopy (SEM) and fourier transform infrared (FTIR) spectroscopy. The results revealed that the viscosity of the mixture solution is directly proportional to its concentration. Increasing the viscosity increased the diameter of fiber but also made the larger beads formation. FTIR measurement exhibited the existence of relevant functional groups of both PVA and chitosan in the composites.The crosslinked structure was found for the electrospun PVA/Chitosan nanofibers treated with glutaraldehyde solution.

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“…The very good biochemical features such as biocompatibility, biodegradability, film forming ability, gelation characteristics, bioadhesion [6] and antitumoral properties [7] of chitosan are well known. Because of its versatility, chitosan was used extensively to coat metallic nanoparticles, including the ones used for hyperthermia applications [6,8], immobilization of enzymes on electrodes in electrochemical biosensors [9], drug delivery for administration of either biomacromolecules or low molecular weight drugs [10], etc. Chitosan is also considered an almost ideal biomaterial for cell culture applications, whereas the cell cultures model represent the most practical approach to evaluate biocompatibility and biotoxicity [11].…”

Section: Introductionmentioning

confidence: 99%

In vitro cytotoxicity of Fe–Cr–Nb–B magnetic nanoparticles under high frequency electromagnetic field

Chiriac

Petreuş

Carasevici

et al. 2015

Journal of Magnetism and Magnetic Materials

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Immobilization of glucose oxidase on chitosan-based porous composite membranes and their potential use in biosensors

Cited by 62 publications

References 28 publications

Bioconversion of d-glucose to d-psicose with immobilized d-xylose isomerase and d-psicose 3-epimerase on Saccharomyces cerevisiae spores

Bioconversion of d-glucose to d-psicose with immobilized d-xylose isomerase and d-psicose 3-epimerase on Saccharomyces cerevisiae spores

Impact of post-treatment on the characteristics of electrospun poly (vinyl alcohol)/chitosan nanofibers

In vitro cytotoxicity of Fe–Cr–Nb–B magnetic nanoparticles under high frequency electromagnetic field

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