Immobilization of glucose oxidase on graphene oxide for highly sensitive biosensors

Hong, Seung No; Kim, Jae Hyun; Kim, Ryang Eun; Kwon, Seok Joo; Kim, Dae Woo; Jung, Hee Tae; Dordick, Jonathan S.; Kim, Jungbae

doi:10.1007/s12257-016-0373-4

Cited by 19 publications

(6 citation statements)

References 26 publications

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“…19 Graphene oxide (GO), a material with high surface area and a structure based on individual nano sheets containing carbonyl, carboxyl, hydroxyl and epoxy groups that confer a good dispersibility in water, has attracted recently much interests as support for enzyme immobilization. [20][21][22][23][24][25][26] GO is commercially available at low price, however its production is mainly based on the oxidation of graphite using an excess of permanganate together with sodium nitrate and concentrated sulphuric acid, that are not green methodologies. Therefore, the use of greener technologies to produce GO are highly desirable.…”

Section: Introductionmentioning

confidence: 99%

Selective synthesis of citrus flavonoids prunin and naringenin using heterogeneized biocatalyst on graphene oxide

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Selective synthesis of citrus flavonoids prunin and naringenin using heterogeneized biocatalyst on graphene oxide

et al. 2019

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“…By using SERS combined with fabricated 3D graphene oxide-(GO)-encapsulated gold nanoparticles, Kim et al reported that differentiated and undifferentiated NSCs can be identified based on differences in the intensities of the Raman peak ( Figure 2 A). GO is preferable because of its ability to adhere to the molecules, which contain high C=C bonds, and its outstanding performance in electrochemical analysis [ 39 ]. The differences in lipid compositions of the cell membranes, which had a C=C bond peak at 1656 cm −1 and a C-H bond peak at 1470 cm −1 , was higher in the undifferentiated cells compared to the differentiated cells ( Figure 2 B–F).…”

Section: Monitoring Nsc Multipotency and Differentiationmentioning

confidence: 99%

Nanobiosensing Platforms for Real-Time and Non-Invasive Monitoring of Stem Cell Pluripotency and Differentiation

Suhito

Angeline

Choo

et al. 2018

Sensors

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Breakthroughs in the biomedical and regenerative therapy fields have led to the influential ability of stem cells to differentiate into specific types of cells that enable the replacement of injured tissues/organs in the human body. Non-destructive identification of stem cell differentiation is highly necessary to avoid losses of differentiated cells, because most of the techniques generally used as confirmation tools for the successful differentiation of stem cells can result in valuable cells becoming irrecoverable. Regarding this issue, recent studies reported that both Raman spectroscopy and electrochemical sensing possess excellent characteristics for monitoring the behavior of stem cells, including differentiation. In this review, we focus on numerous studies that have investigated the detection of stem cell pluripotency and differentiation in non-invasive and non-destructive manner, mainly by using the Raman and electrochemical methods. Through this review, we present information that could provide scientific or technical motivation to employ or further develop these two techniques for stem cell research and its application.

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“…Moreover, such immobilization could be achieved through one or in combination with others. Hong et al ( 2016 ) developed biosensors based on GOD immobilized on graphene oxide (GRO) through various preparative approaches including enzyme adsorption (EA), enzyme adsorption and cross-linking (EAC), and enzyme adsorption, precipitation and cross-linking (EAPC). One of the most important aspects of such GOD based immobilization is the greater loading of enzymes for the efficient functioning which makes the overall system stable and selective in the form of cross-linking precipitated GOD aggregates.…”

Section: Applications Of God In Various Sectorsmentioning

confidence: 99%

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

et al. 2017

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Fungal glucose oxidase (GOD) is widely employed in the different sectors of food industries for use in baking products, dry egg powder, beverages, and gluconic acid production. GOD also has several other novel applications in chemical, pharmaceutical, textile, and other biotechnological industries. The electrochemical suitability of GOD catalyzed reactions has enabled its successful use in bioelectronic devices, particularly biofuel cells, and biosensors. Other crucial aspects of GOD such as improved feeding efficiency in response to GOD supplemental diet, roles in antimicrobial activities, and enhancing pathogen defense response, thereby providing induced resistance in plants have also been reported. Moreover, the medical science, another emerging branch where GOD was recently reported to induce several apoptosis characteristics as well as cellular senescence by downregulating Klotho gene expression. These widespread applications of GOD have led to increased demand for more extensive research to improve its production, characterization, and enhanced stability to enable long term usages. Currently, GOD is mainly produced and purified from Aspergillus niger and Penicillium species, but the yield is relatively low and the purification process is troublesome. It is practical to build an excellent GOD-producing strain. Therefore, the present review describes innovative methods of enhancing fungal GOD production by using genetic and non-genetic approaches in-depth along with purification techniques. The review also highlights current research progress in the cost effective production of GOD, including key advances, potential applications and limitations. Therefore, there is an extensive need to commercialize these processes by developing and optimizing novel strategies for cost effective GOD production.

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Immobilization of glucose oxidase on graphene oxide for highly sensitive biosensors

Cited by 19 publications

References 26 publications

Selective synthesis of citrus flavonoids prunin and naringenin using heterogeneized biocatalyst on graphene oxide

Selective synthesis of citrus flavonoids prunin and naringenin using heterogeneized biocatalyst on graphene oxide

Nanobiosensing Platforms for Real-Time and Non-Invasive Monitoring of Stem Cell Pluripotency and Differentiation

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current Updates

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