Immobilization of heavy metal contaminated mine wastes using Canavalia ensiformis extract

Nam, In-Hyun; Roh, Seung-Bum; Park, Min-Jeong; Chon, Chul-Min; Kim, Jae-Gon; Jeong, Sangbae; Song, Hocheol; Yoon, Min-Ho

doi:10.1016/j.catena.2015.07.019

Cited by 33 publications

(5 citation statements)

References 49 publications

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“…The results indicated that the retention efficiency of the remediated soil can be improved by 30% in comparison with the unremediated soil. Nam et al (2016) examined the ability of the Canavalia ensiformis extract to catalyze urea to form carbonate precipitation on heavy metals resulting from abandoned mines and employed X-ray diffraction and scanning electron microscopy (SEM) to confirm the precipitation speciation. The findings of this work suggested that such an extract is effective in immobilizing heavy metals and preventing their diffusion into surrounding environments.…”

Section: Introductionmentioning

confidence: 99%

Effects of the Urease Concentration and Calcium Source on Enzyme-Induced Carbonate Precipitation for Lead Remediation

et al. 2022

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Heavy metal contamination during the rapid urbanization process in recent decades has notably impacted our fragile environments and threatens human health. However, traditional remediation approaches are considered time-consuming and costly, and the effect sometimes does not meet the requirements expected. The present study conducted test tube experiments to reproduce enzyme-induced carbonate precipitation applied to lead remediation under the effects of urease concentration and a calcium source. Furthermore, the speciation and sequence of the carbonate precipitation were simulated using the Visual MINTEQ software package. The results indicated that higher urease concentrations can assure the availability of CO32− during the enzyme-induced carbonate precipitation (EICP) process toward benefiting carbonate precipitation. The calcium source determines the speciation of carbonate precipitation and subsequently the Pb remediation efficiency. The use of CaO results in the dissolution of Pb(OH)2 and, therefore, discharges Pb ions, causing some difficulty in forming the multi-layer structure of carbonate precipitation and degrading Pb remediation. The findings of this study are useful in widening the horizon of applications of the enzyme-induced carbonate precipitation technology to heavy metal remediation.

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Section: Introductionmentioning

confidence: 99%

Effects of the Urease Concentration and Calcium Source on Enzyme-Induced Carbonate Precipitation for Lead Remediation

et al. 2022

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“…NADH dependent glutamate dehydrogenase also use ammonia released after urease activity and can be measured spectrophotometrically and specific electrodes are also available for detection and monitoring of ammonia release (Dilrukshi and Kawasaki, 2016). Gel electrophoresis method is also used in which sample with urease is electrophoresed on acrylamide and agarose gel and active protein can be detected after incubation of gel in solution of phenol red and urea and this assay can help in protein size estimation (Nam et al, 2016). Some common quantitative assays like phenolhypochlorite assays, in which ammonia reacts with phenol hypochlorite and results in the formation of indophenol which can be easily measured spectrophotometrically and even a small amount of ammonia is detectable by this process, but the samples prepared were mainly sensitive to temperature, pH buffer and inhibitors (Hashihama et al, 2015).…”

Section: Urease Assaymentioning

confidence: 99%

“…Fig.1. Different assays for detection of urease(Braissant et al, 2020, Toth et al, 2018, Dong et al, 2017, Hashihama et al, 2015, Nam et al, 2016, Dilrukshi and Kawasaki, 2016, Chang et al, 2017 …”

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confidence: 99%

A review on distribution, properties, genetic organization, immobilisation and applications of urease

Kumar

Bhardwaj²,

Yadav³

et al. 2022

JANS

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Urease, a nickel-containing metalloenzyme is getting remarkable attention due to a diverse range of applications for mankind. Urease plays a magnificent role in various field like agriculture, analytical, geological phenomena, beverage industry and is an important diagnostic tool. Urease is mainly present in bacteria, fungi, plants and invertebrates and its manifestation in specific genera may open new vistas for its taxonomic position. Various qualitative and quantitative assays are also reported for the estimation of urease enzyme. Urease based biosensors utilizing green synthesis on nanoparticles are also trending. Recently developed inhibitors against urease were discussed in the review. Inhibitory mechanisms involving the structural similarity of the substrate through modification or derivatization can also help in rational drug design by two possible competitive ways either by mimicking monodentate urea binding or binding as a tetrahedral intermediate. Immobilisation of urease through gel entrapment, using non-covalent and covalent protein tags, cross linkage, covalent bonding, using composite films, Teflon, co-precipitation and coating on nanoparticles is also reported. This review also comprised of various application of urease including enhancement of fertility in the soil, cell to cell organization, protection to predators, treatment of various bladder related diseases and infections, analysis of urea and heavy metal ions, biocementation, pollution control by bioleaching of heavy metals and making beverages urea and ethyl carbamate free. As researchers have a keen interest in urease enzyme at present, most of its aspects were incorporated in the article to make it helpful to the scientific community for further research related to the development of new inhibitors and add on applications of urease for the upliftment of the human as well as environment.

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“…Nam, Roh [130] applied the EICP technique to immobilize and remove heavy metals and metalloids in contaminated mine wastes. The results from their study indicated that the concentrations of As, Mn, Zn, Pb, Cr and Cu were reduced by 31.7%, 65.8%, 50.6%, 51.6%, 45.1% and 49.7%, respectively.…”

Section: Removal Of Heavy Metalsmentioning

confidence: 99%

A Review of Enzyme Induced Carbonate Precipitation (EICP): The Role of Enzyme Kinetics

Ahenkorah

Rahman

Karim

et al. 2021

Sustainable Chemistry

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Enzyme-induced carbonate precipitation (EICP) is a relatively new bio-cementation technique for ground improvement. In EICP, calcium carbonate () precipitation occurs via urea hydrolysis catalysed by the urease enzyme sourced from plants. EICP offers significant potential for innovative and sustainable engineering applications, including strengthening of soils, remediation of contaminants, enhancement of oil recovery through bio-plugging and other in situ field applications. Given the numerous potential applications of EICP, theoretical understanding of the rate and quantity of precipitation via the ureolytic chemical reaction is vital for optimising the process. For instance, in a typical EICP process, the rate and quantity of precipitation can depend significantly on the concentration, activity and kinetic properties of the enzyme used along with the reaction environment such as pH and temperature. This paper reviews the research and development of enzyme-catalysed reactions and its applications for enhancing precipitation in EICP. The paper also presents the assessment and estimation of kinetic parameters, such as the maximal reaction velocity () and the Michaelis constant (), that are associated with applications in civil and geotechnical engineering. Various models for evaluating the kinetic reactions in EICP are presented and discussed, taking into account the influence of pH, temperature and inhibitors. It is shown that a good understanding of the kinetic properties of the urease enzyme can be useful in the development, optimisation and prediction of the rate of precipitation in EICP.

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Immobilization of heavy metal contaminated mine wastes using Canavalia ensiformis extract

Cited by 33 publications

References 49 publications

Effects of the Urease Concentration and Calcium Source on Enzyme-Induced Carbonate Precipitation for Lead Remediation

Effects of the Urease Concentration and Calcium Source on Enzyme-Induced Carbonate Precipitation for Lead Remediation

A review on distribution, properties, genetic organization, immobilisation and applications of urease

A Review of Enzyme Induced Carbonate Precipitation (EICP): The Role of Enzyme Kinetics

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