Immobilization of horse liver alcohol dehydrogenase on copolymers of glycidyl methacrylate and ethylene dimethacrylate

Abstract

Alcohol dehydrogenase has been immobilized to the basic copolymer and its several derivatives using various techniques. Enzyme coupling to the supports with amino groups by means of glutaraldehyde was found the most suitable. Activity of alcohol dehydrogenase coupled to these amino supports was comparable to that of the enzyme bound to Sepharose. Thermal and pH stability of alcohol dehydrogenase increased essentially upon immobilization. Kinetic properties of the immobilized enzyme differed from those of free alcohol dehydrogenase, pH optimum shifted to alkaline range, and apparent Michaelis constants for substrates and coenzymes increased. Curvatures observed in Lineweaver-Burk plots for coenzymes suggest an involvement of diffusion effects in the reaction catalyzed by alcohol dehydrogenase linked to these polymers.

Redoks Polimerizasyonu ile Epoksi Reçine İçeren Poli(GMA-b-EG) Blok Kopolimerinin Sentezi ve Karakterizasyonu

“…GMA-based copolymers are of great importance in biotechnological applications since the epoxy group can be easily converted to other functional groups. In addition, homogeneous and heterogeneous polymer networks can be prepared with GMA used in coatings, matrix resins and adhesives (Wang et al, 2022;Liu et al, 2022;Waly et al, 2020;Klimow et al, 2022;Wang & Hutchinson, 2008;Muzammil et al, 2017;May, 1988;Matt et al,2021;Kovar et al, 1982;Yılmaz & Coşkun, 1982). Polyethylene glycol (PEG) is a straight or branched chain polymer in which oxygen and ethylene units are sequentially bonded to each other.…”

Porous polyelectrolyte materials with controlled luminescence properties based on aromatic‐aromatic interactions with rhodamine B

“…Porous materials are interesting due to a number of applications related to surface phenomena. In fact, the low mass and high surface area are prominent characteristics of these materials that allow good performance for molecular adsorption and exchange, 18‐20 catalytic functionalization, 21,22 absorption of pollutant liquids, 23 among others. Freeze‐drying constitutes a simple and easy methodology to produce porous monoliths from aqueous solutions of polyelectrolytes by means of the ice segregation induced self‐assembly (ISISA) phenomena 24,25 .…”

Effect of photo-crosslinking on the performance of silica nanoparticle-filled epoxidized acrylic copolymer coatings

“…4 Such structural features of epoxies thereby confer thermal stability, resistance to solvents, chemicals, moisture and salt sprays, adhesion to a broad variety of substrates including aluminum, magnesium, steel, titanium, beryllium, copper, most alloys and plastics, all of which makes them ideal candidates as coatings, adhesives and encapsulants. 5,6 Among the epoxy entities, glycidyl methacrylate (GMA)-based copolymers have attracted great interest in epoxy resin technologies due to their easy copolymerization with other unsaturated comonomers, [7][8][9][10] and most importantly the straightforward conversion of the epoxy group into various functional groups 11,12 permitting the preparation of homogenous and heterogeneous polymer networks with tailored properties. [13][14][15][16] On other hand, and in order to improve the intrinsic properties of thermosets, organic or inorganic llers are generally incorporated using different methods.…”