Immobilization of horseradish peroxidase in three-dimensional macroporous TiO2 matrices for biosensor applications

Zhu, Yihua; Cao, Huimin; Tang, Longhua; Yang, Xin; Li, Chunzhong

doi:10.1016/j.electacta.2008.11.025

Cited by 68 publications

(33 citation statements)

References 25 publications

(25 reference statements)

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“…Organic membranes show good chemical and thermal stability improved by an inorganic phase. For example, chitosan (CS) was usually combined with TiO 2 nanoparticles [8,9], ZnO nanoparticles [10,11], and carbon nanotubes [12,13]. Nowadays, there are few reports about titanium dioxide-multiwall carbon nanotubes-chitosan (TiO 2 -MWNTs-CS) composite.…”

Section: Introductionmentioning

confidence: 99%

Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles

Zhang

Yuan

et al. 2011

Bioprocess Biosyst Eng

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In this paper, a new glucose biosensor was prepared. At first, Prussian blue (PB) was electrodeposited on a glassy carbon electrode (GCE) modified by titanium dioxide-multiwall carbon nanotubes-chitosan (TiO(2)-MWNTs-CS) composite, and then gold nanoparticles functionalized by poly(diallyldimethylammonium chloride) (PDDA-Au) were adsorbed on the PB film. Finally, the negatively charged glucose oxidase (GOD) was self-assembled on to the positively charged PDDA-Au. The electrochemical performances of the modified electrodes had been studied by cyclic voltammetry (CV) and amperometric methods, respectively. In addition, the stepwise fabrication process of the as-prepared biosensor was characterized by scanning electron microscopy. PDDA-Au nanoparticles were characterized by ultraviolet-vis absorption spectroscopy and transmission electron microscopy. Under the optimal conditions, the as-prepared biosensor exhibited a good response performance to glucose with a linear range from 6 μM to 1.2 mM with a detection limit of 0.1 μM glucose (S/N = 3). In addition, this work indicated that TiO(2)-MWNTs-CS composite and PDDA-Au nanoparticles held great potential for constructing biosensors.

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Section: Introductionmentioning

confidence: 99%

Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles

Zhang

Yuan

et al. 2011

Bioprocess Biosyst Eng

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“…3DOM Prussian blue (PB) film [15] and 3DOM gold film [16] modified electrodes have also been prepared for glucose detection. Li et al immobilized HRP onto the 3DOM titania modified electrode, and developed a highly sensitive photoelectric sensor for the determination of H 2 O 2 [17] . In these systems, the film efficiency or sensitivity was significantly higher than that of nonporous films.…”

Section: Introductionmentioning

confidence: 99%

Preparation of the glucose sensor based on three-dimensional ordered macroporous gold film and room temperature ionic liquid

Chen

Xuan

Jiang

et al. 2009

Sci. China Ser. B-Chem.

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A novel type of glucose sensor was fabricated based on a glucose oxidase (GOD)-N,N-dimethtylformamide (DMF)-[BMIm][BF 4 ] composites modified three-dimensional ordered macroporous (3DOM) gold film electrode. The immobilized GOD exhibits a pair of well-defined reversible peaks in 50 mM pH 7.0 phosphate buffer solutions (PBS), which could be attributed to the redox of flavin adenine dinucleotide (FAD) in GOD. The research results show that ionic liquid ([BMIm][BF 4 ]), DMF and 3DOM gold film are crucial for GOD to exhibit a pair of stable and reversible peaks. It is believed that the large active area of 3DOM gold film can increase the amount of immobilized GOD. Simultaneously, the application of IL enhances the stability of GOD and facilitates the electron transfer between GOD and the electrode. The synergetic effect of DMF can help the GOD to maintain its bioactivity better. GOD immobilized on the electrode exhibits the favorable electrocatalytic property to glucose, and the prepared sensor has a linear range from 10 to 125 nM with a detection limit of 3.3 nM at a signal-to-noise ratio of 3σ. The apparent K m (Michaelis-Menten constant) for the enzymatic reaction is 0.018 mM.ionic liquid, glucose oxidase, three-dimensional ordered macroporous gold film, direct electrochemistry, electrochemical catalysis

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“…Due to its good biocompatibility, high conductivity and low cost, TiO 2 in various forms such as nanoparticles, nanoneedles and nanotubes, has become an attractive electrode material for electrochemical sensors and biosensors applications [17][18][19][20][21][22][23][24]. Recently, Liu et al fabricated the electrochemical sensor by casting TiO 2 nanotubes film onto glassy carbon electrode (GCE) surface.…”

Section: Introductionmentioning

confidence: 99%

TiO2-graphene nanocomposite for electrochemical sensing of adenine and guanine

Fan

Huang

Niu

et al. 2011

Electrochimica Acta

189

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a b s t r a c tTiO 2 -graphene nanocomposite was prepared by hydrolysis of titanium isopropoxide in colloidal suspension of graphene oxide and in situ hydrothermal treatment. It provides an efficient and facile approach to yield nanocomposite with TiO 2 nanoparticles uniformly embedded on graphene substrate. The electrochemical behavior of adenine and guanine at the TiO 2 -graphene nanocomposite modified glassy carbon electrode was investigated. The results show that the incorporation of TiO 2 nanoparticles with graphene significantly improved the electrocatalytic activity and voltammetric response towards these species comparing with that at the graphene film. The TiO 2 -graphene based electrochemical sensor exhibits wide linear range of 0.5-200 M with detection limit of 0.10 and 0.15 M for adenine and guanine detection, respectively. The excellent performance of this electrochemical sensor can be attributed to the high adsorptivity and conductivity of TiO 2 -graphene nanocomposite, which provides an efficient microenvironment for electrochemical reaction of these purine bases.

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Immobilization of horseradish peroxidase in three-dimensional macroporous TiO2 matrices for biosensor applications

Cited by 68 publications

References 25 publications

Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles

Glucose biosensor based on titanium dioxide-multiwall carbon nanotubes-chitosan composite and functionalized gold nanoparticles

Preparation of the glucose sensor based on three-dimensional ordered macroporous gold film and room temperature ionic liquid

TiO2-graphene nanocomposite for electrochemical sensing of adenine and guanine

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