Immobilization of horseradish peroxidase on electrospun poly(vinyl alcohol)–polyacrylamide blend nanofiber membrane and its use in the conversion of phenol

Temoçin, Zülfikar; İnal, Murat; Gökgöz, Murat; Yi̇ği̇toğlu, Mustafa

doi:10.1007/s00289-017-2129-5

Cited by 51 publications

(17 citation statements)

References 77 publications

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“…For instance, using commercially available biogenic porous silica, Sahare et al found a SF of 23[45] with an immobilized preparation with similar specific activity to the one used in our work. HRP immobilized onto PVA–PAAm nanofibers was found to retain 64% of its initial activity at 4°C after 55 days which represents a lower storage stability compared to the nanohybrid prepared herein[46]. For each of these examples it is important to highlight that the nature of the support used and the chemistry of HRP immobilization is different for that developed in this work.…”

Section: Resultsmentioning

confidence: 99%

Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP

et al. 2019

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Hybrid and composite nanoparticles represent an attractive material for enzyme integration due to possible synergic advantages of the structural builders in the properties of the nanobiocatalyst. In this study, we report the synthesis of a new stable hybrid nanobiocatalyst formed by biomimetic silica (Si) nanoparticles entrapping both Horseradish Peroxidase (HRP) (EC 1.11.1.7) and magnetic nanoparticles (MNPs). We have demonstrated that tailoring of the synthetic reagents and post immobilization treatments greatly impacted physical and biocatalytic properties such as an unprecedented ~280 times increase in the half-life time in thermal stability experiments. The optimized nanohybrid biocatalyst that showed superparamagnetic behaviour, was effective in the batch conversion of indole-3-acetic acid, a prodrug used in Direct Enzyme Prodrug Therapy (DEPT). Our system, that was not cytotoxic per se , showed enhanced cytotoxic activity in the presence of the prodrug towards HCT-116, a colorectal cancer cell line. The strategy developed proved to be effective in obtaining a stabilized nanobiocatalyst combining three different organic/inorganic materials with potential in DEPT and other biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP

et al. 2019

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“…Moreover, it prevents interactions with interfaces, improves resistance to inhibitors and product purity (Barbosa et al., 2015; Bilal et al., 2018; Cleiton et al., 2015; Rodrigues et al., 2013; Sheldon & Brady, 2018; Sheldon & van Pelt, 2013; Sheldon & Pereira, 2017). For efficient catalysis and yield, the immobilization material must be selected carefully based on its robustness, biocompatibility and recoverability (Temocin et al., 2018). Over the years, lipase immobilization on various support materials such as silica gel particles (Lee et al., 2006; Yang et al., 2010), macroporous resin (Yang et al., 2006), silica nanoparticles (Babaki et al., 2016), nanozeolites (Vasconcellos et al., 2015), carbon nanotubes (Fan et al., 2016), hybrid nanospheres (Verri et al., 2016) and chitosan microspheres (Palla et al., 2011) have been investigated for various uses.…”

Section: Enzymatic Synthesis Of Opomentioning

confidence: 99%

1,3‐Dioleoyl‐2‐palmitoyl glycerol (OPO)—Enzymatic synthesis and use as an important supplement in infant formulas

Ghide

Yan

2021

J Food Biochem

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1,3‐dioleolyl‐2‐palmitate (OPO) is an important component of the human milk fat. Its unique fatty acid composition and distribution play an important role in proper infant growth and development. Owing to this, it has been attracting researchers and manufacturers to synthesize and commercialize OPO as an important human milk fat substitute added to infant formulas. In this review, the role of OPO in human milk, the benefits of OPO (sn‐2 palmitate)‐supplemented infant formulas over the conventional infant formulas on infant growth, and lipase‐catalyzed synthesis of OPO are discussed. Over the last 20 years of research on the benefits of OPO (sn2 palmitate)‐supplemented infant formulas are summarized. Similarly, studies carried out on lipase catalyzed production of OPO for the last 21 years (1999–2019) are also done focusing on the raw materials, sn1,3‐regiospecific lipases, immobilization materials, and solvents used in the laboratory‐scale experiments. In addition, OPO‐based products currently in the market and future research trends are briefly covered in this review. Practical applications This work focuses on lipase‐catalyzed synthesis of 1,3‐dioleoyl‐2‐palmitoylglycerol (the most abundant triacyl glycerol in human milk fat) and its benefits to infants when it is added in infant formulas. Over the last 20 years of published research from the literature are summarized and future research trends for efficient OPO synthesis are also covered. This will provide current and future researchers on the field with the necessary background information on OPO synthesis and design their research plans accordingly for cost‐effective production of OPO and OPO‐supplemented infant formulas.

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“…A wide range of polymers are used to enzyme immobilization, and poly(ε-caprolactone) [16] and poly(vinyl alcohol) are one of them [17]. It is worth to paying attention that during the production of electrospun nanofibers it is possible to obtain composite which, having many functional groups, increase the affinity of the enzyme to the support, for example polycaprolactone/cellulose monoacetate [18] and poly(vinyl alcohol)/polyacrylamide [19].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability

Zdarta

Jankowska

Wyszowska

et al. 2019

Materials Science and Engineering: C

100

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Immobilization of horseradish peroxidase on electrospun poly(vinyl alcohol)–polyacrylamide blend nanofiber membrane and its use in the conversion of phenol

Cited by 51 publications

References 77 publications

Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP

Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP

1,3‐Dioleoyl‐2‐palmitoyl glycerol (OPO)—Enzymatic synthesis and use as an important supplement in infant formulas

Robust biodegradation of naproxen and diclofenac by laccase immobilized using electrospun nanofibers with enhanced stability and reusability

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