Immobilization of <i>β</i>‐Glucosidase BglC on Decanedioic Acid‐Modified Magnetic Nanoparticles

Wang, Nuo; Wu, Rina; Fu, Qiang; Wang, Hongling; Zhang, Zheng; Haji, Ze; Li, Xiu-Tong; Lian, Xinglong; An, Yi

doi:10.1002/ceat.201700477

Cited by 12 publications

(4 citation statements)

References 22 publications

(20 reference statements)

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“…As shown in Figure 5, bgl and CelDZ1 retained more than 70% of their initial activity after 24 days, while the residual activity of both GOx and HRP was found to be around 30% of their initial activity. Other studies have also mentioned enhanced storage stability (up to 7 weeks during storage at 4 • C) for individually immobilized GOx [43] and cellulase onto magnetic nanoparticles [44] as well as for bgl immobilized on modified magnetic nanoparticles [45]. Increased storage stability has also been reported for the co-immobilization of cellulase on magnetic nanoparticles, either with α-amylase and pectinase or with pectinase and xylanase [17,18].…”

Section: Storage Stability Studiesmentioning

confidence: 99%

Development of a Four-Enzyme Magnetic Nanobiocatalyst for Multi-Step Cascade Reactions

et al. 2019

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We report the preparation, characterization and application of a novel magnetic four-enzyme nanobiocatalyst prepared by the simultaneous covalent co-immobilization of cellulase (CelDZ1), β-glucosidase (bgl), glucose oxidase (GOx) and horseradish peroxidase (HRP) onto the surface of amino-functionalized magnetic nanoparticles (MNPs). This nanobiocatalyst was characterized by various spectroscopic techniques. The co-immobilization process yielded maximum recovered enzymatic activity (CelDZ1: 42%, bgl: 66%, GOx: 94% and HRP: 78%) at a 10% v/v cross-linker concentration, after 2 h incubation time and at 1:1 mass ratio of MNPs to total enzyme content. The immobilization process leads to an increase of Km and a decrease of Vmax values of co-immobilized enzymes. The thermal stability studies of the co-immobilized enzymes indicated up to 2-fold increase in half-life time constants and up to 1.5-fold increase in their deactivation energies compared to the native enzymes. The enhanced thermodynamic parameters of the four-enzyme co-immobilized MNPs also suggested increment in their thermal stability. Furthermore, the co-immobilized enzymes retained a significant part of their activity (up to 50%) after 5 reaction cycles at 50 °C and remained active even after 24 d of incubation at 5 °C. The nanobiocatalyst was successfully applied in a four-step cascade reaction involving the hydrolysis of cellulose.

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Section: Storage Stability Studiesmentioning

confidence: 99%

Development of a Four-Enzyme Magnetic Nanobiocatalyst for Multi-Step Cascade Reactions

et al. 2019

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“…It should be pointed out that the n-hexane adsorption capacity of BC/ PU foam composites may be affected by many factors, such as BC content, foam structure, BC characteristics (particle size, surface acidic functional groups, etc.) and experimental conditions (Wang et al 2018b). The negative value of ΔG 0 (− 4.4 kJ•mol −1 , 298 K) implied that the adsorption process of BC/PU-5 to OSs was energetically conducive to spontaneous occurrence.…”

Section: Adsorption Isotherms With Bc/pu-5 Foam Compositesmentioning

confidence: 99%

Bamboo charcoal fused with polyurethane foam for efficiently removing organic solvents from wastewater: experimental and simulation

Wang

Zhao

et al. 2022

Biochar

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The development of a multifunctional oil adsorbing material which could effectively and quickly separate oily wastewater is one of the focuses in water environment restoration. In this study, bamboo charcoal (BC) was used as an improver to modify polyurethane (PU) foam. The results of scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR) revealed that the addition of BC could effectively improve the mechanical properties of PU. The adsorption data exhibited that the BC-loaded PU (BC/PU) foam composites effectively removed seven organic solvents (OSs, including octane, petroleum ether, soybean oil, chlorobenzene, 1,2-dichloroethane, n-hexane, cyclohexane), and the maximum adsorption capacity of BC/PU was 23.6 g g−1 when BC content was 5%. The order of pseudo-second-order kinetic constants and maximum adsorption capacity of seven OSs was octane < petroleum ether < soybean oil < chlorobenzene < 1, 2-dichloroethane < cyclohexane < n-hexane. Based on the experimental data and density functional theory (DFT) simulation, the adsorption mechanism of OSs on BC/PU-5 was discussed. The EHOMO and μ of OSs calculated by DFT were highly correlated with absorption affinity (K2, Qe and Qmax). Hence, the contribution of OSs to the adsorption efficiency of BC/PU-5 may be mainly due to electron donor–acceptor (EDA) interaction and non-hydrophobic interaction. In addition, the adsorption capacity did not change significantly after repeated recycling 5 times. Overall, the prepared BC/PU foam composites could be used as a potential candidate for separating OSs in engineering applications. Graphical Abstract

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“…The optimal reaction pH value was examined by 0.5 p-NPG as a substrate at 60 °C in different pH values (from 4 to 8). The β-glucosidase activity was calculated by measuring the absorbance at 425 nm using a Multiskan GO microplate spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) [ 29 ].…”

Section: Methodsmentioning

confidence: 99%

Production of Siamenoside I and Mogroside IV from Siraitia grosvenorii Using Immobilized β-Glucosidase

et al. 2022

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Siraitia grosvenorii is a type of fruit used in traditional Chinese medicine. Previous studies have shown that the conversion of saponins was often carried out by chemical hydrolysis, which can be problematic because of the environmental hazards it may cause and the low yield it produces. Therefore, the purpose of this study is to establish a continuous bioreactor with immobilized enzymes to produce siamenoside I and mogroside IV. The results show that the immobilization process of β-glucosidase exhibited the best relative activity with a glutaraldehyde (GA) concentration of 1.5%, carrier activation time of 1 h and binding enzyme time of 12 h. After the immobilization through GA linkage, the highest relative activity of β-glucosidase was recorded through the reaction with the substrate at 60 °C and pH 5. Subsequently, the glass microspheres with immobilized β-glucosidase were filled into the reactor to maintain the optimal active environment, and the aqueous solution of Siraitia grosvenorii extract was introduced by controlling the flow rate. The highest concentration of siamenoside I and mogroside IV were obtained at a flow rate of 0.3 and 0.2 mL/min, respectively. By developing this immobilized enzyme system, siamenoside I and mogroside IV can be prepared in large quantities for industrial applications.

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Immobilization of β‐Glucosidase BglC on Decanedioic Acid‐Modified Magnetic Nanoparticles

Cited by 12 publications

References 22 publications

Development of a Four-Enzyme Magnetic Nanobiocatalyst for Multi-Step Cascade Reactions

Development of a Four-Enzyme Magnetic Nanobiocatalyst for Multi-Step Cascade Reactions

Bamboo charcoal fused with polyurethane foam for efficiently removing organic solvents from wastewater: experimental and simulation

Production of Siamenoside I and Mogroside IV from Siraitia grosvenorii Using Immobilized β-Glucosidase

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