Immobilization of laccase onto 1-aminopyrene functionalized carbon nanotubes and their electrocatalytic activity for oxygen reduction

Pang, Haili; Liu, J.; Hu, Dan; Zhang, X.H.; Chen, J.H.

doi:10.1016/j.electacta.2010.06.013

Cited by 66 publications

(41 citation statements)

References 49 publications

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“…Another approach includes a crosslinking molecule that binds to carbon nanotubes through non-covalent functionalization such as hydrophobic and pep interactions [58,59], and covalently bind the protein through, for example, an amide bond. For example, a bifunctional pyrene molecule can irreversibly adsorb through pep stacking and be used as a reactive linker for bioconjugation [46,60].…”

Section: Covalent Linkingmentioning

confidence: 99%

Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

191

111

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a b s t r a c tSince the discovery of low-dimensional carbon allotropes, there is increasing interest in using carbon nanomaterials for biomedical applications. Carbon nanomaterials have been utilized in the biomedical field for bioimaging, chemical sensing, targeting, delivery, therapeutics, catalysis, and energy harvesting. Each application requires tailored surface functionalization in order to take advantage of a desired property of the nanoparticles. Herein, we review the surface immobilization of bio-molecules, including proteins, peptides, and enzymes, and present the recent advances in synthesis and applications of these conjugates. The carbon scaffold and the biological moiety form a complex interface which presents a challenge for achieving efficient and robust binding while preserving biological activity. Moreover, some applications require the utilization of the protein-nanocarbon system in a complex environment that may hinder its performance or activity. We analyze different strategies to overcome these challenges when using carbon nanomaterials as protein carriers, explore various immobilization techniques along with characterization methods, and present recent demonstrations of employing these systems for biomedical applications. Finally, we consider the challenges and future directions of this field.

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Section: Covalent Linkingmentioning

confidence: 99%

Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

191

111

View full text Add to dashboard Cite

show abstract

“…This method was recently applied for the immobilization of laccase onto CNTs, although using an amino terminated pyrene derivative [24].…”

Section: Introductionmentioning

confidence: 99%

Enhanced direct electron transfer between laccase and hierarchical carbon microfibers/carbon nanotubes composite electrodes. Comparison of three enzyme immobilization methods

Gutiérrez-Sánchez

Jia

Beyl

et al. 2012

Electrochimica Acta

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“…IV characteristics with a sharp increase in a narrow range of relative pressure (P/P o = 0.2-0.3), because of capillary condensation of N 2 in the hexagonal channels (Pang, Liu, Hu, Zhang, & Chen, 2010). As can be seen in Table 1, once MCM is functionalized with hyperbranched PEI, the "a s , BET " significantly decreases.…”

Section: Characterization Of MCM and Mcm@peimentioning

confidence: 95%