Immobilization of lipase on surface modified magnetic nanoparticles using alkyl benzenesulfonate

Abstract

The surface of nano-sized magnetite (NSM) particles synthesized by coprecipitation method was modified by alkyl benzenesulfonate (ABS) as a coating material. ABS on the NSM was expected to form a spacer between the surface of the NSM particles and the enzyme adsorbed and to play a role of strong enzyme adsorption onto a hydrophobic surface. Transmission electron microscopy showed that the NSM particles had an average size of 10 nm. Magnetic measurement revealed that the nanoparticles were superparamagnetic and the saturation magnetization was about 68 emu/g. Porcine pancreas lipase (PPL) was immobilized onto the ABS-NSM, which was to catalyze hydrolysis of olive oil and showed enhanced durability in the reuse after being recovered by magnetic separations.

Fabrication of a magnetic nanoparticle embedded NH2-MIL-88B MOF hybrid for highly efficient covalent immobilization of lipase

“…As one of the most widely used biocatalysts, CRL has drawn much attention because of broad diversity of reactions such as enantioselective hydrolysis, esterification, chiral resolution, and trans-esterification. [12][13] On the other hand, covalent binding is a better approach to immobilize lipase which can prevent the leaching from the support. To overcome the above problems, immobilization of lipase on a support is considered to be a promising approach to enhance the catalytic stability, feasible continuous operations, easy recycling, and significant reduction of costs and so on.…”

Covalent immobilization of porcine pancreatic lipase on carboxyl-activated magnetic nanoparticles: Characterization and application for enzymatic inhibition assays

“…Up to date, PPL has been immobilized with various solid supports including macroporous anion exchange resin, magnetic microspheres, chitosan beads, porous silica beads and polyvinyl alcohol [3,7,8]. The critical shortcoming of physical adsorption is that the immobilized enzymes are easily leached out from porous materials, resulting in enzyme loss and poor operational stability [9,10]. Covalent immobilization can bind enzymes to the solid supports stably, thus preventing leakage of the enzymes.…”

Fabrication of a Chitosan‐Coated Magnetic Nanobiocatalyst for Starch Hydrolysis

“…Nanoparticles have the potential to serve as a superior solid matrix for enzyme immobilization due to their high surface area compared with traditional support materials 2. Magnetic nanoparticles (MNPs) have attractive characteristics like high surface area for enzyme loading, nontoxicity, lower mass transfer resistance, and efficient separation of small solid particles from the reaction mixture by the external magnetic field 18–22. However, enzymes immobilized onto MNPs via physical adsorption showed reduced stability after repeated use 23, 24.…”