Immobilization of liposomes onto quartz crystal microbalance to detect interaction between liposomes and proteins

Morita, Shigeyuki; Nukui, Miho; Kuboi, Ryoichi

doi:10.1016/j.jcis.2005.12.043

Cited by 52 publications

(44 citation statements)

References 36 publications

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“…Probably due to these complicated factors, the liquidphase based method has showed poor reproducibility. However, this liquid-phase based procedure is actually the most appropriate method for revealing the biomolecular interaction in the solution (Morita et al, 2006). In this work, the static liquid-phase measurement was adopted to keep the background buffer in an immobile state, so the ΔF ρ has been minimized and almost has no influence on the ΔF.…”

Section: Immunosensor Fabrication 1 Detailed Fabrication and Analytimentioning

confidence: 99%

Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

Wang

et al. 2008

J. Zhejiang Univ. Sci. B

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By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid (MHDA) was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide (NHS) raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor's resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0×10 2 colony forming units (CFU)/ml E. coli O157:H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly (LBL-SA) method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed.

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Section: Immunosensor Fabrication 1 Detailed Fabrication and Analytimentioning

confidence: 99%

Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

Wang

et al. 2008

J. Zhejiang Univ. Sci. B

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“…Monolayers and SLBs have been used due to their simplicity and reproducibility [4,5]. However, liposomes are recognized to reproduce better the native cell membrane's fluidity and mobility due to their three-dimensional spherically closed structure [6,7].…”

Section: Introductionmentioning

confidence: 99%

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Serro

Galante

Kozica

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Different types of lipid bilayers/monolayers have been used to simulate the cellular membranes in the investigation of the interactions between drugs and cells. However, to our knowledge, very few studies focused on the influence of the chosen membrane model upon the obtained results. The main objective of this work is to understand how do the nature and immobilization state of the biomembrane models influence the effect of the local anaesthetic tetracaine (TTC) upon the lipid membranes. The interaction of TTC with different biomembrane models of dimyristoylphosphatidylcholine (DMPC) with and without cholesterol (CHOL) was investigated through several techniques. A quartz crystal microbalance with dissipation (QCM-D) was used to study the effect on immobilized liposomes, while phosphorus nuclear magnetic resonance ( 31 P-NMR) and differential scanning calorimetry (DSC) were applied to liposomes in suspension. The effect of TTC on Langmuir monolayers of lipids was also investigated through surface pressure-area measurements at the air-water interface. The general conclusion was that TTC has a fluidizing effect on the lipid membranes and, above certain concentrations, induced membrane swelling or even solubilization. However, different models led to variable responses to the TTC action. The intensity of the disordering effect caused by TTC increased in the following order: supported liposomes < liposomes in solution < Langmuir monolayers. This means that extrapolation of the results obtain in in vitro studies of the lipid/anaesthetic interactions to in vivo conditions should be done carefully.

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“…In a previous report, the amino group of phosphoethanolamine mixed in the liposomal membrane easily condensed on activated carboxyl group on QCM electrode, followed by the immobilization of the liposome 17) . In this study, using a one-step condensate agent DMT-MM 18,19) , the condensation between amino groups of self-assembled monolayers and carboxyl group of fatty acid in liposomal membrane without the activation process was attempted.…”

Section: Immobilization Of Liposomes Onto Electrode Of Qcmmentioning

confidence: 97%

“…The interaction between A β and 1,2-dimyristoyl-sn-glycero-3-phosphocholine/ fatty acid liposome was quantitatively characterized by using liposome-immobilized quartz crystal microbalance method 17) , together with the measurement of compression isotherms of mixed DMPC and fatty acid monolayers. Alternatively, monodisperse LUVs were prepared using an extruder (LiposoFast TM , Avestin Inc., Ottawa, Canada) equipped with polycarbonate filters possessing pore sizes of 100 nm.…”

Section: Issue For Membrane Stress Biotechnologymentioning

confidence: 99%

Effect of Fatty Acids on Interaction between Liposome and Amyloid β-peptide

2007

Self Cite

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In 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC)/fatty acid (FA) system, the analysis of the interaction between the DMPC/FA liposome and amyloid β-peptide (Aβ) was carried out by using the quartz crystal microbalance (QCM) method, together with the analysis of the compression isotherms of the DMPC/FA monolayer. The DMPC/stearic acid (SA) liposome showed the highest intensity in its interaction with Aβ through the QCM method although the slight interaction between DMPC/linoleic acids (LAs) liposome and Aβ was obtained. The compression isotherm analysis of the lipid monolayers revealed that the DMPC/SA monolayers underwent the distinct phase transfer, with which the interaction between both molecules changed from repulsive to attractive, while DMPC/linoleic acid monolayers did not.

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Immobilization of liposomes onto quartz crystal microbalance to detect interaction between liposomes and proteins

Cited by 52 publications

References 36 publications

Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

Effect of tetracaine on DMPC and DMPC+cholesterol biomembrane models: Liposomes and monolayers

Effect of Fatty Acids on Interaction between Liposome and Amyloid β-peptide

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