Immobilization of magnetic modified Flavobacterium ATCC 27551 using magnetic field and evaluation of the enzyme stability of immobilized bacteria

Robatjazi, Seyed Mortaza; Shojaosadati, Seyed Abbas; Khalilzadeh, Rassoul; Vasheghani‐Farahani, Ebrahim; Balochi, Nooshin

doi:10.1016/j.biortech.2011.11.035

Cited by 23 publications

(17 citation statements)

References 25 publications

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“…The interest in the immobilization of microorganisms and microbial enzymes for biotechnological applications has been continuously rising during the last decades because of several factors, including the increased availability of microbial strains and biocatalysts tailored to new applications, the development of new immobilization supports with improved properties, and the need of a shift toward the use of more sustainable processes in different industrial fields [1][2][3][4][5]. The immobilization of microorganisms and enzymes on solid carriers leads to a number of benefits.…”

Section: Introductionmentioning

confidence: 99%

“…and transformation processes in multiple fields (e.g., agricultural, environmental, food, medical, etc.) has been explored as well to enhance the microbial biological activity, to facilitate their delivery and to separate them more easily from the fermentation broth [3,[5][6][7][8]. Therefore, during the last years there has been an emerging interest in biocompatibility studies for interfacing biological systems with artificial materials.…”

Section: Introductionmentioning

confidence: 99%

“…During the last 40 years, a range of different materials have been investigated as enzyme and microbial immobilization matrices: from organic compounds, like natural alginate or carrageenan or synthetic polymers, to inorganic compounds, such as processed or natural minerals, like silica [3,9]. In the last decade, the focus has been put in the use of nanocomposites as promising immobilization matrices.…”

Section: Introductionmentioning

confidence: 99%

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Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

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et al. 2019

IJMS

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The ability of commercial monolayer graphene oxide (GO) and graphene oxide nanocolloids (GOC) to interact with different unicellular systems and biomolecules was studied by analyzing the response of human alveolar carcinoma epithelial cells, the yeast Saccharomyces cerevisiae and the bacteria Vibrio fischeri to the presence of different nanoparticle concentrations, and by studying the binding affinity of different microbial enzymes, like the α-l-rhamnosidase enzyme RhaB1 from the bacteria Lactobacillus plantarum and the AbG β-d-glucosidase from Agrobacterium sp. (strain ATCC 21400). An analysis of cytotoxicity on human epithelial cell line A549, S. cerevisiae (colony forming units, ROS induction, genotoxicity) and V. fischeri (luminescence inhibition) cells determined the potential of both nanoparticle types to damage the selected unicellular systems. Also, the protein binding affinity of the graphene derivatives at different oxidation levels was analyzed. The reported results highlight the variability that can exist in terms of toxicological potential and binding affinity depending on the target organism or protein and the selected nanomaterial.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Domi

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et al. 2019

IJMS

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“…S. fuliginis ATCC 27551 has the ability to degrade a broad spectrum of organophosphorus pesticides and insecticides, which are of great environmental concern due to their presence on contaminated soils, sediments and 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 groundwater 33 . For this reason, the immobilization of this strain for bioremediation purposes on magnetic nanoparticles, through covalent binding and ionic adsorption, has been studied 34 . The ability of E. coli, P. putida and B. diminuta strains to develop biofilms has been described before, but knowledge on S. fuliginis biofilm formation capacity in any surface type is very scarce.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria

Tamayo-Ramos

Rumbo

Caso³

et al. 2018

ACS Appl. Mater. Interfaces

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Polymeric electrospun fibers are becoming popular in microbial biotechnology because of their exceptional physicochemical characteristics, biodegradability, surface-to-volume ratio, and compatibility with biological systems, which give them a great potential as microbial supports to be used in production processes or environmental applications. In this work, we analyzed and compared the ability of Escherichia coli, Pseudomonas putida, Brevundimonas diminuta, and Sphingobium fuliginis to develop biofilms on different types of polycaprolactone (PCL) microfibers. These bacterial species are relevant in the production of biobased chemicals, enzymes, and proteins for therapeutic use and bioremediation. The obtained results demonstrated that all selected species were able to attach efficiently to the PCL microfibers. Also, the ability of pure cultures of S. fuliginis (former Flavobacterium sp. ATCC 27551, a very relevant strain in the bioremediation of organophosphorus compounds) to form dense biofilms was observed for the first time, opening the possibility of new applications for this microorganism. This material showed to have a high microbial loading capacity, regardless of the mesh density and fiber diameter. A comparative analysis between PCL and polylactic acid (PLA) electrospun microfibers indicated that both surfaces have a similar bacterial loading capacity, but the former material showed higher resistance to microbial degradation than PLA.

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“…Utilization of immobilized cells eliminates time-consuming and cost-intensive steps involved in the isolation and purification of intracellular enzymes [1]. Immobilization with biocompatible carriers prevents microbial cells from being diluted in an open-water system and extends the production capacity of microbial cells [2].…”

Section: Introductionmentioning

confidence: 99%

Nitric Acid-Treated Carbon Fibers with Enhanced Hydrophilicity for Candida tropicalis Immobilization in Xylitol Fermentation

et al. 2016

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Nitric acid (HNO3)-treated carbon fiber (CF) rich in hydrophilic groups was applied as a cell-immobilized carrier for xylitol fermentation. Using scanning electron microscopy, we characterized the morphology of the HNO3-treated CF. Additionally, we evaluated the immobilized efficiency (IE) of Candida tropicalis and xylitol fermentation yield by investigating the surface properties of nitric acid treated CF, specifically, the acidic group content, zero charge point, degree of moisture and contact angle. We found that adhesion is the major mechanism for cell immobilization and that it is greatly affected by the hydrophilic–hydrophilic surface properties. In our experiments, we found 3 hto be the optimal time for treating CF with nitric acid, resulting in an improved IE of Candida tropicalis of 0.98 g∙g−1 and the highest xylitol yield and volumetric productivity (70.13% and 1.22 g∙L−1∙h−1, respectively). The HNO3-treated CF represents a promising method for preparing biocompatible biocarriers for multi-batch fermentation.

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Immobilization of magnetic modified Flavobacterium ATCC 27551 using magnetic field and evaluation of the enzyme stability of immobilized bacteria

Cited by 23 publications

References 25 publications

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Interaction Analysis of Commercial Graphene Oxide Nanoparticles with Unicellular Systems and Biomolecules

Analysis of Polycaprolactone Microfibers as Biofilm Carriers for Biotechnologically Relevant Bacteria

Nitric Acid-Treated Carbon Fibers with Enhanced Hydrophilicity for Candida tropicalis Immobilization in Xylitol Fermentation

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