Immobilization of pamidronic acids on the nanotube surface of titanium discs and their interaction with bone cells

Abstract

Self-assembled layers of vertically aligned titanium nanotubes were fabricated on a Ti disc by anodization. Pamidronic acids (PDAs) were then immobilized on the nanotube surface to improve osseointegration. Wide-angle X-ray diffraction, X-ray photoelectron microscopy, and scanning electron microscopy were employed to characterize the structure and morphology of the PDA-immobilized TiO2 nanotubes. The in vitro behavior of osteoblast and osteoclast cells cultured on an unmodified and surface-modified Ti disc was examined in terms of cell adhesion, proliferation, and differentiation. Osteoblast adhesion, proliferation, and differentiation were improved substantially by the topography of the TiO2 nanotubes, producing an interlocked cell structure. PDA immobilized on the TiO2 nanotube surface suppressed the viability of the osteoclasts and reduced their bone resorption activity.

Targeting and molecular imaging of HepG2 cells using surface-functionalized gold nanoparticles

“…A third peak with lower intensity near 284.72 eV is assigned to the C-S on the surface of the GL. The peaks around 285.77 and 288.70 eV are assigned to C-C and -CONH-, indicating successful immobilization of lactobionic acid on the surface of GE (Koo et al 2013). …”

Titania Nanotubes for Local Drug Delivery from Implant Surfaces

“…Bisphosphonates represent another class of growth factors that prevent bone loss and are widely used to treat conditions like bone metastasis, Paget's disease etc. [117]. In separate studies, bisphosphonates pamidronate and ibandronate were successfully loaded inside TNTs in an attempt to reduce bone resorption activity [117,118].…”

“…[117]. In separate studies, bisphosphonates pamidronate and ibandronate were successfully loaded inside TNTs in an attempt to reduce bone resorption activity [117,118]. Upon implantation in tibias of Wistar rats, anodised and heat treated Ti surface with ibandronate were compared with machined Ti, anodised and heat treated Ti [118].…”

Growth Behavior of TiO2 Nanotube Arrays in Different Electrolyte pH

“…Ti implants are commonly used in orthopedics and dentistry for their favorable biocompatibility and corrosion resistance [1]. Upon exposed to the atmosphere, the Ti metal spontaneously forms a thin, dense and protective oxide layer (approximately 10 nm thick) on its surface, which act like a ceramic with superior biocompatibility.…”