Immobilization of Pepsin on Chitosan Magnetic Nanoparticles and Its Application in Deacetylation of Amides

Magnetic Fe3O4@MCM-41 core–shell nanoparticles functionalized with thiol silane for efficient l-asparaginase immobilization

“…The DSC curve (Figure 4) for all samples showed an endothermic peak at about 100 C, which are related to the evaporation of the surface water. The exothermic peak between 250 C and 270 C was due to the oxidation and change in crystallinity of Fe 3 O 4 [44]. Apart from these peak, no significant peaks were detected for both the Fe 3 O 4 and Fe 3 O 4 @MCM-41.…”

Pepsin immobilization by electrospinning of poly(vinyl alcohol) nanofibers

“…9 Pepsin specializes in the cleavage of bonds involving aromatic aminoacid residues, for example: tyrosine, phenylalanine and tryptophan. 10,11 Due to its effectiveness and broad specificity, pepsin has found applications in the treatment and manufacturing of food like cheese preparation and related milk-containing foods. 7,8,10 In laboratory, pepsin peptidase is mostly used in the analysis of proteins, mainly through online protein digestion.…”

“…7,8,10 In laboratory, pepsin peptidase is mostly used in the analysis of proteins, mainly through online protein digestion. [10][11][12] Other applications of this important enzyme are in the leather industry, affinity chromatography and bioreactors. 11 However, as an isolated enzyme, pepsin shows limitations of reusability and lack of stability at harsh and unnatural reaction mediums that lead to the loss of its tridimensional protein structure by denaturation process.…”

“…[10][11][12] Other applications of this important enzyme are in the leather industry, affinity chromatography and bioreactors. 11 However, as an isolated enzyme, pepsin shows limitations of reusability and lack of stability at harsh and unnatural reaction mediums that lead to the loss of its tridimensional protein structure by denaturation process. 1 Hence, there have been several attempts to enhance the catalytic behavior of pepsin, mainly through several encapsulation and immobilization methods that help to maintain its natural tridimensional proteinic structure after the extraction from its natural medium.…”

“…Among the different supports reported for pepsin immobilization we can find polymeric supports, 7,[13][14][15][16] inorganic scaffolds [17][18][19] and several nanostructures. 11,20,21 However, most of these immobilization methods are complicated and require several steps to achieve the immobilization of pepsin. In this context, as nanometric support, the nanofibers have been proven to be a reliable, effective and biocompatible method for the encapsulation and immobilization of several important enzymes, for instance: cyclodextrin glucanotransferase, α-amylase, cellulase, lysozyme, lipase, trypsin, tyrosinase, laccase and glucose oxidase.…”