IMMOBILIZATION OF Saccharomyces cerevisiae IN RICE HULLS FOR ETHANOL PRODUCTION

Martini, Edita; Andriani, Dian; Gobikrishnan, Sriramulu; Kang, Kyoung Ho; Bark, Surn-Teh; Sunwoo, Changshin; Prasetya, Bambang; Park, Don-Hee

doi:10.7454/mst.v14i2.693

Cited by 3 publications

(5 citation statements)

References 12 publications

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“…The immobilized cells on sDPPF gave 6.8% higher ethanol yield than that of free cells. The result of this study was 10.6% higher than those reported by Martini et al (2010) whose results immobilized Saccharomyces cerevisiae on rice hulls for ethanol production (Table 3). Generally, the performance of ethanol production by immobilized cells adhesion onto the surface of the carrier is much better than free cells because the immobilization system protects the cells from inhibition of ethanol by biofilm formation (Yao et al 2011).…”

Section: Discussioncontrasting

confidence: 75%

Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843

Riansa-ngawong¹,

Suwansaard²,

Prasertsan³

2012

Electron. J. Biotechnol.

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Palm pressed fiber (PPF) is a clean and renewable lignocellulosic material. The PPF and delignified PPF (DPPF) were used as a carrier for immobilization of Candida shehatae TISTR5843 in bioethanol production. PPF was pre-treated by milling to obtain small particles, whereas DPPF was the delignification of PPF using NaClO2. C. shehatae TISTR5843 was grown in modified yeast extractmalt (YM) medium at 30 ± 2ºC on an orbital shaker at 150 rpm for batch and repeated batch fermentation. In the batch system, immobilized cells on a small size, less than 0.5 mm, of DPPF (sDPPF) gave the maximum ethanol production of 11.5 g L -1 at 24 hrs cultivation period. The ethanol concentration and ethanol yield of sDPPF were 6.2% and 6.8% higher (ethanol production 11.5 g L -1 , ethanol yield 0.47 g g -1 ) than those of free cells (ethanol production 10.8 g L -1 , ethanol yield 0.44 g g -1 ) after 36 hrs of cultivation. In contrast, the small size of PPF (sPPF) was selected as a carrier in repeated batch fermentation for cost effectiveness. The ethanol productivity of immobilized yeast cells in repeated batch fermentations was 45.2-51.6% greater than that obtained from batch fermentations. The immobilized cells on sPPF improved the ethanol production and could be reused 4 times with retaining the activity of 93%. In conclusion, PPF is a potential carrier in the immobilization system. The pre-treatment of PPF increases the surface area that enhances cell adsorption and ethanol production by C. shehatae TISTR5843.

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Section: Discussioncontrasting

confidence: 75%

Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843

Riansa-ngawong¹,

Suwansaard²,

Prasertsan³

2012

Electron. J. Biotechnol.

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“…The decreased concentration of produced acetlyglucosamine in the second cycle of fermentation was also found in a previous research ( 40 ) using immobilized Saccharomyces cerevisiae in rice hulls for ethanol production. This might be caused by leaching of immobilized cells from the pumice stone surface into the fermentation medium due to abrasion effect.…”

Section: Resultssupporting

confidence: 84%

“…This might be caused by leaching of immobilized cells on the pumice stone's surface into the fermentation medium due to abrasion effect. This phenomenon leads to a reduction of the number of cells immobilized in the pumice stones, leaving mostly the cells which were entrapped within the pores (39). There is a possibility that some of the N-acetylglucosamine produced in the first cycle might possibly be contributed by the free cells which were leaked from the pumice stones (19).…”

Section: Resultsmentioning

confidence: 99%

Immobilization of Providencia stuartii Cells in Pumice Stone and Its Application for N-Acetylglucosamine Production

Halim

Devianita

Hardoko

et al. 2021

Food Technol. Biotechnol. (Online)

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Research background. Shrimp shells contain chitin that can further be processed into N-acetylglucosamine which has been extensively used to treat joint damage. Providencia stuartii isolated form previous research has strong chitinolytic activity and may be utilized in the form of immobilized cells to be used in repeated fermentation. Pumice is a porous and rigid stone that offers superior mechanical strength, making it suitable to be used for immobilization process. Experimental approach. The research used experimental method to conduct the submerged fermentation process with different pumice stone size and pumice stone:growth medium ratio (m/V). The fermentation was carried out for 4 days at 37 C and pH of 7.0. The optimum pumice stone size and pumice stone:growth medium ratio (m/V) were used to determine the optimum fermentation cycle to produce N-acetylglucosamine. Results and conclusions. Pumice stones of 1.0×1.0×1.0 cm and pumice stone:growth medium ratio (m/V) of 1:5 were found to be the optimum conditions which successfully immobilized (89.99±1.65) % cells and produced (331.37±7.34) g/L N-acetylglucosamine. The highest N-acetylglucosamine concentration of (322.97±2.46) g/L was obtained in the first fermentation cycle which then decreased and remained stable throughout the last three cycles of fermentation. Novelty and scientific contribution. P. stuartii was a strong chitinolytic bacteria previously isolated from rotten shrimp shells and was used for the first time in immobilized form to produce N-acetylglucosamine. The findings in this research showed potential use of P. stuartii cells immobilized in pumice stone for continuous production of N-acetylglucosamine using fermentation method.

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“…The ratio between the weight of sugarcane bagasse with the inoculum volume of Saccharomyces cerevisiae in the process of cell immobilization and the percentage of biocatalyst to the volume of fermentation medium are very important to understand. It aims to facilitate the cell adhesion and to scale up the process and the production of bioethanol [14]. This paper describes the potential use of sugarcane bagasse as an alternative carrier for cell immobilization and improvement in the production process of cell immobilization in bagasse.…”

Section: Introductionmentioning

confidence: 99%

“…Many reports have suggested various immobilization carriers for bioethanol fermentation, such as sorghum bagasse [9,12], orange peel [13], rice hulls [14], corn cobs [15], sugar beet pulp [16], palm pressed fiber [17], cork (Sonneratia caseolaris) root [18], and sugarcane bagasse [8].…”

Section: Introductionmentioning

confidence: 99%

Sugarcane Bagasse as a Carrier for the Immobilization of Saccharomyces cerevisiaein Bioethanol Production

Anita

Mangunwardoyo

Yopi

2016

MST

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Sugarcane bagasse was used as a carrier to immobilize Saccharomyces cerevisiae in bioethanol production. This research aims to study the potential use of sugarcane bagasse as an alternative carrier for cell immobilization and improvement in the production process of cell immobilization in bagasse. The results showed that the physical characteristics of sugarcane bagasse as a carrier were water content (7.77 ± 0.35%), water retention (4.80 ± 0.44 g/g), water absorption index (8.58 ± 0.22 g/g), and lignin content (24.40 ± 1.52 %). Determination of cell retention was performed in an inoculum volume of 50 mL yeast suspension with various carrier weights (2.5, 5, 10, and 20 g). The highest cell retention was obtained in ratio of 2.5 g carrier/50 mL cell suspension with cell retention of 5.41 ± 1.06 mg/g, or known as biocatalyst. Biocatalyst, as much as 1.5, 3, 4.5, and 6 g, were used as inoculum for a 24 hour bioethanol fermentation. The best concentration and productivity of bioethanol, obtained by using 3 g of biocatalyst, were 23.95 ± 0.28 g/L and 1.24 ± 0.01 g/L/hours. The average of bioethanol yield for a 24 hour fermentation by using immobilized cells was three times higher than the free cells system. AbstrakBagas sebagai Carrier untuk Imobilisasi Saccharomyces cerevisiae pada Produksi Bioetanol. Bagas digunakan sebagai carrier untuk imobilisasi pada produksi bioetanol. Penelitian bertujuan untuk mengetahui potensi penggunaan bagas sebagai carrier alternatif untuk imobilisasi sel dan perbaikan proses pembuatan imobilisasi sel pada bagas. Hasil penelitian menunjukkan bahwa karakteristik fisik yang dimiliki bagas sebagai carrier yaitu kadar air (7,77 ± 0,35%), retensi air (4,80 ± 0,44 g/g), indeks penyerapan air (8,58 ± 0,22 g/g), dan kadar lignin (24,40 ± 1,52%). Pengukuran retensi sel dilakukan pada variasi berat carrier (2,5, 5, 10, dan 20 g) dengan volume inokulum sebanyak 50 mL suspensi sel. Retensi sel tertinggi, diperoleh pada perbandingan 2,5 g carrier/50 mL suspensi sel, yaitu 5,41 ± 1,06 mg/g dan disebut sebagai biokatalis. Biokatalis sebanyak 1,5; 3; 4,5; dan 6 g digunakan sebagai inokulum untuk fermentasi bioetanol selama 24 jam. Konsentrasi dan produktivitas bioetanol terbaik menggunakan 3 g biokatalis yaitu 23,95 ± 0,28 g/L dan 1,24 ± 0,01 g/L/jam. Rendemen bioetanol selama 24 jam fermentasi menggunakan sel terimobilisasi adalah tiga kali lebih tinggi dibandingan dengan sel bebas.

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IMMOBILIZATION OF Saccharomyces cerevisiae IN RICE HULLS FOR ETHANOL PRODUCTION

Cited by 3 publications

References 12 publications

Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843

Application of palm pressed fiber as a carrier for ethanol production by Candida shehatae TISTR5843

Immobilization of Providencia stuartii Cells in Pumice Stone and Its Application for N-Acetylglucosamine Production

Sugarcane Bagasse as a Carrier for the Immobilization of Saccharomyces cerevisiaein Bioethanol Production

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