Immobilization of Thiol-Modified Horseradish Peroxidase on Gold Nanoparticles Enhances Enzyme Stability and Prevents Proteolytic Digestion

Breausche, Faith E.; Somerlot, Annelise; Walder, Jason; Osei, Kwame; Okyem, Samuel; Driskell, Jeremy D.

doi:10.1021/acs.langmuir.4c01180

Langmuir

2024

DOI: 10.1021/acs.langmuir.4c01180

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Immobilization of Thiol-Modified Horseradish Peroxidase on Gold Nanoparticles Enhances Enzyme Stability and Prevents Proteolytic Digestion

Faith E. Breausche,

Annelise Somerlot,

Jason Walder

et al.

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Cited by 3 publications

References 62 publications

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Covalent immobilization: A review from an enzyme perspective

Prabhakar,

Giaretta,

Zulli

et al. 2025

Chemical Engineering Journal

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Covalent immobilization: A review from an enzyme perspective

Prabhakar,

Giaretta,

Zulli

et al. 2025

Chemical Engineering Journal

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Enhanced Biodiesel Production with Eversa Transform 2.0 Lipase on Magnetic Nanoparticles

dos Santos,

de França Serpa,

de Castro Bizerra

et al. 2024

Langmuir

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This research investigated the usefulness of magnetic iron oxide nanoparticles (Fe 3 O 4 ) as a support to immobilize the lipase Eversa Transform 2.0 (ET 2.0) to obtain an active and stable biocatalyst, easily recoverable from the reaction medium for applications in the production of biodiesel. Biodiesel was an alternative fuel composed mainly of fatty acid esters with strong transesterification and esterification capabilities. The study focused on the esterification of oleic acid with ethanol to synthesize ethyl oleate. Magnetic nanoparticles were prepared by coprecipitation, then activated with glutaraldehyde and functionalized with γ-aminopropyltriethoxysilane (APTES). The optimal conditions for immobilizing ET 2.0 were pH 10, 25 mM sodium carbonate buffer, an enzymatic load of 200 U/g, and 1 h of contact time, obtaining 78% yield and enzymatic activity of 205.9 U/g. Postimmobilization evaluation showed that the immobilized enzyme performed better than its free form. Kinetic studies were conducted under these optimized conditions (2−96 h at 150 rpm and 37 °C). The biocatalyst was tested for the synthesis of ethyl oleate using oleic acid as the substrate and ethanol, achieving a conversion of 88.1%. Subsequent recirculation tests maintained approximately 80% conversion until the fourth cycle, confirming the sustainability of ester production. Molecular docking studies revealed that the binding affinity for the enzyme-docked oil composition was estimated at −5.8 kcal/mol, suggesting that the combination of the substrate and lipase was stable and suitable for esterification.

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Immobilization of Bromelain on Gold Nanoparticles for Comprehensive Detection of Their Antioxidant, Anti-Angiogenic, and Wound-Healing Potentials

Ausaj,

Al-Shmgani,

Abid

et al. 2024

Inorganics

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Bromelain (Br) is a proteolytic enzyme with various pharmacological properties, such as antibacterial, antioxidant, anti-inflammatory, anticancer, and anti-angiogenic properties. However, due to its low solubility and bioavailability, its absorption is low, so a delivery mechanism is needed to achieve the desired therapeutic effects. Bromelain was chemically synthesized and loaded onto gold nanoparticles (AuNPs). Different methods and techniques were used for detection and characterization, including color-change detection, UV spectroscopy, XRD, SEM, TEM, and FTIR. The in vitro antioxidant activity was detected using DPPH assays, and the wound-healing activity was investigated in mice. The current study revealed that the formulated AuNPs-Br showed effective antioxidant activity and the strongest wound-healing properties, as demonstrated by histopathological and in vivo studies, and showed anti-angiogenic effects.

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Immobilization of Thiol-Modified Horseradish Peroxidase on Gold Nanoparticles Enhances Enzyme Stability and Prevents Proteolytic Digestion

Cited by 3 publications

References 62 publications

Covalent immobilization: A review from an enzyme perspective

Covalent immobilization: A review from an enzyme perspective

Enhanced Biodiesel Production with Eversa Transform 2.0 Lipase on Magnetic Nanoparticles

Immobilization of Bromelain on Gold Nanoparticles for Comprehensive Detection of Their Antioxidant, Anti-Angiogenic, and Wound-Healing Potentials

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