Immobilization of β-galactosidase on modified polypropilene membranes

Vasileva, N.; Iotov, Vladislav; Ivanov, Yavor; Godjevargova, Tzonka; Kotia, Nana

doi:10.1016/j.ijbiomac.2012.07.032

Cited by 19 publications

(6 citation statements)

References 39 publications

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“…Therefore according to the results, enzyme with the highest specific activity of 195.2 Umg -1 proteins was immobilized at the optimal medium temperature of 20°C. The decrease with increasing medium temperature is consistent with the study conducted by Vasileva et al 8 They used modified polypropylene membrane as a support material for enzyme immobilization. Amount of bound protein and enzyme activity was recorded to decrease above 4°C.…”

Section: Effect Of Process Parameters On Immobilization Efficiency Ansupporting

confidence: 91%

β-Galactosidase Immobilization on Chitosan-Hydroxyapatite Complex: Effects of Immobilization Conditions

Çabuk

Tarı

Harsa

2014

JNHFE

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Preparation of glutaraldehyde activated chitosanhydroxyapatite (chitosan-HAP) microspheres As supports for enzyme immobilization, equal amounts of powdered chitosan and HAP were dissolved in 1M of 100ml acetic acid solution. This solution was added drop wise through a syringe into gently stirred 1.5% w/v sodium tripolyphosphate crosslinking solution. Thereafter, the beads were washed with deionized water until neutrality. For activation, chitosan-HAP beads were incubated

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Section: Effect Of Process Parameters On Immobilization Efficiency Ansupporting

confidence: 91%

β-Galactosidase Immobilization on Chitosan-Hydroxyapatite Complex: Effects of Immobilization Conditions

Çabuk

Tarı

Harsa

2014

JNHFE

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“…Enzymatic activity evolution was represented by the classical Michaelis-Menten Equation (1) [13,22] and is represented in Figure 3. Then, kinetics parameters were determined using the LineweaverBurk representation for the immobilized and free enzyme (Figure 3 A decrease of only 40% of the enzymatic activity was observed after three runs, followed by good stability in the subsequent runs.…”

Section: Kinetic Parameters Of Immobilized β-Galmentioning

confidence: 99%

Investigation of the Effect of Plasma Polymerized Siloxane Coating for Enzyme Immobilization and Microfluidic Device Conception

et al. 2016

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This paper describes the impact of a physical immobilization methodology, using plasma polymerized 1,1,3,3, tetramethyldisiloxane, on the catalytic performance of β-galactosidase from Aspergillus oryzae in a microfluidic device. The β-galactosidase was immobilized by a polymer coating grown by Plasma Enhanced Chemical Vapor Deposition (PEVCD). Combined with a microchannel patterned in the silicone, a microreactor was obtained with which the diffusion through the plasma polymerized layer and the hydrolysis of a synthetic substrate, the resorufin-β-D-galactopyranoside, were studied. A study of the efficiency of the immobilization procedure was investigated after several uses and kinetic parameters of immobilized β-galactosidase were calculated and compared with those of soluble enzyme. Simulation and a modelling approach were also initiated to understand phenomena that influenced enzyme behavior in the physical immobilization method. Thus, the catalytic performances of immobilized enzymes were directly influenced by immobilization conditions and particularly by the diffusion behavior and availability of substrate molecules in the enzyme microenvironment.

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“…For the immobilization of unmodified enrofloxacin, on one hand epoxy and aldehyde surfaces were modified with dihydrazide (protocol adapted from: Vasileva et al 2012, Alves et al 2009 [17,18]), on the other hand poly-L-lysine surfaces were used. For the modification with adipic acid dihydrazide, the epoxy slides were first EDC activated, by immersing in a solution of 50mM EDC in 1x PBS (pH 7.4).…”

Section: Surface Functionalizationsmentioning

confidence: 99%

Influence of different surface chemistries on the ultrasensitive on-chip detection of enrofloxacin in milk

Poller

Crooks

Preininger

2015

Sensors and Actuators B: Chemical

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Immobilization of β-galactosidase on modified polypropilene membranes

Cited by 19 publications

References 39 publications

β-Galactosidase Immobilization on Chitosan-Hydroxyapatite Complex: Effects of Immobilization Conditions

β-Galactosidase Immobilization on Chitosan-Hydroxyapatite Complex: Effects of Immobilization Conditions

Investigation of the Effect of Plasma Polymerized Siloxane Coating for Enzyme Immobilization and Microfluidic Device Conception

Influence of different surface chemistries on the ultrasensitive on-chip detection of enrofloxacin in milk

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