Immobilization of β‐glucosidase from different sources on nylon tube

Abstract

Cellulase from four different fungi and B-glucosidase from almonds were immobilized on the inner surface of nylon tubing. The highest values of P-glucosidase activity retention on the support were obtained when P. friniculosurn and N. crassa were used as the enzyme source. A comparative study of the thermal stability referring to B-glucosidase activity was developed using free and immobilized enzymes. The most stable fi-glucosidases (from P. funiculosum and A. niger) did not show an appreciable change in its thermal stability after immobilization. An important increase in thermal stability was observed when less stable P-glucosidases (from T. reesei, N. crassa and almonds) were immobilized.

Thermal Deactivation of Free and Immobilized β‐Glucosidase from Penicillium funiculosum

“…Despite this interest, relatively few studies have been published on the thermal deactivation of @-glucosidase (Gianfreda et al, 1985;Yazdi et al, 1989;Woodward et al, 1992) and, in particular, on the cellobiose hydrolysis reaction kinetics considering thermoinactivation of the catalyst (Bravo et al, 1990;Caminal et al, 1985). In this paper, we report studies of the thennoinactivation of the /?-glucosidase activity in a commercial cellulase from Penicillium funiculosum.…”

Smectite Clays as Solid Supports for Immobilization of β-Glucosidase: Synthesis, Characterization, and Biochemical Properties

“…4 Immobilized glucosidases are utilized in many large-scale processes, mainly because they allow for catalyst recycling, continuous operation, and controlled product formation. Adsorption via covalent binding to solid supports, entrapment in polymer hydrogels, and microencapsulation are common methods to immobilize glucosidases from different sources to various supports including resins, 1 calcium alginate gels, 5 agarose gels, 6 silica gel, 7 carbon nanotubes, 8 and polymer carriers such as nylon 9,10 and Eupergit C. 11 Obviously, the structural characteristics of the support are important since they may influence the catalytic behavior and stability of the immobilized enzyme. 12 Reduction of dimensions of enzymecarrier materials can generally improve the catalytic efficiency of immobilized enzymes.…”