Immobilization of β-glucosidase onto Magnetic Nanoparticles and Evaluation of the Enzymatic Properties

Abstract

This paper reports on a novel and efficient β-glucosidase immobilization method using magnetic Fe 3 O 4 nanoparticles as a carrier. Based on response surface methodology, the optimal immobilization conditions obtained were: glutaraldehyde (GA) concentration, 0.20%; enzyme concentration, 50.25 μg/mL; cross-linking time, 2.21 h; and the maximum activity recovery reached 89.35%. The magnetic immobilized enzyme was characterized by Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM), and vibrating sample magnetometer (VSM). FTIR revealed that β-glucosidase was successfully immobilized on the magnetic nanoparticles. TEM showed that enzyme-magnetic nanoparticles possessed nano-scale size distribution. VSM confirmed that the enzyme-magnetic nanoparticles were superparamagnetic. The properties of the immobilized β-glucosidase were improved, and the immobilized β-glucosidase exhibited wider pH and temperature ranges of activation, higher accessibility of the substrate, better thermal stability, and better storage stability than that of the free enzyme. The enzyme-magnetic nanoparticles could be separated magnetically for easy reuse. Immobilization of β-glucosidase onto the magnetic nanoparticles has the potential for industrial application.

Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties

“…The shift of the optimum temperature to higher values may be attributed to conformational changes of the enzyme upon immobilization (ElGhaffar and Hashem, 2010). A similar increase of the optimum temperature for the catalytic activity of immobilized bgl on various supports has been also reported previously (Zhou et al, 2013;Song et al, 2016).…”

“…enzyme was observed at pH 5.0; a similar pH profile has been reported for the immobilization of bgl on iron magnetic nanoparticles (Abraham et al, 2014), while other studies demonstrated that immobilized bgl on magnetic nanoparticles reaches its maximum activity at pH 4.0 (Ahmed et al, 2013;Zhou et al, 2013). In most cases, the immobilized bgl was found to keep its activity in a pH range from 2.5 to 5.0, indicating that the immobilized enzyme is less sensitive to the modification of the pH than the free enzyme.…”

Novel microbial and root mediated green synthesis of TiO2 nanoparticles and its application in wastewater remediation

“…The broad peak in the 800–400 cm ‐1 range is due to Ti‐O and Ti‐O‐Ti vibrations . The peaks near 1543 and 1652 cm ‐1 can be assigned to –N‐H bending and carbonyl stretching vibrations of the glucosidase enzyme . MF2 exhibits similar FTIR spectra whereas in the case of MF3 and MFc, apart from the broad peak for O‐H stretching and Ti‐O, the prominent peaks seen are at 1543 and 1652 cm ‐1 suggesting the involvement of glucosidase enzyme in the synthesis.…”

Immobilization of β‐Glucosidase BglC on Decanedioic Acid‐Modified Magnetic Nanoparticles

“…However, the high cost of recovery of enzymes for repeated applications is a limitation to the wide application of β ‐glucosidase. Immobilization of β ‐glucosidase is considered to be an efficient method to overcome this drawback . A lot of researchers have focused on β ‐glucosidase immobilization.…”

“…Using magnetic nanoparticles for enzyme immobilization has attracted increasing attention because of their large surface area and easy isolation from the reaction solution under magnetic force . β ‐Glucosidase has also been immobilized on magnetic nanoparticles such as magnetic nanoparticles coupled with agarose , poly( N , N '‐methylenebisacrylamide‐co‐glycidyl methacrylate) (PMG) core/shell magnetic nanoparticles , and aminated and starch‐coated magnetic nanoparticles . Although the catalytic properties of enzymes in organic solvents are very important for industrial application, improved activities of these immobilized enzymes in organic solvents have never been reported.…”