Immobilized artificial membrane chromatography: Rapid purification of functional membrane proteins

Pidgeon, Charles; Stevens, Joan; Otto, Stephanie; Jefcoate, Colin R.; Marcus, Carol S.

doi:10.1016/0003-2697(91)90164-o

Cited by 54 publications

(18 citation statements)

References 34 publications

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“…In the case of the RPE65 results reported here, this putative vesiphy (24) is a solid-phase membrane mimetic system that has been successfully used to purify a number of cle aggregation is not accompanied by vesicle fusion such as is seen with the annexin family of proteins (33). membrane proteins including cytochrome P450s (24) and cholesterol-transfer protein (25). In IAM supports, Vesicle fusion was assayed for by resonance energy transfer (15).…”

Section: Discussionmentioning

confidence: 99%

RPE65, the Major Retinal Pigment Epithelium Microsomal Membrane Protein, Associates with Phospholipid Liposomes

Tsilou

Hamel

et al. 1997

Archives of Biochemistry and Biophysics

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The photoreceptor neurons of the vertebrate retina sociated with RPE microsomal membranes. To explore and the retinal pigment epithelium (RPE) 3 constitute a the possible involvement of interactions with phos-complex that is highly interdependent developmentally pholipids, an isotonic salt-soluble extract of RPE was and functionally. Both contain highly specialized memincubated with phosphatidylcholine (PC)/phosphati-brane components central to their common purpose of dylserine (PS)/phosphatidylinositol liposomes and maintaining the visual cycle: the RPE with its extencentrifuged to sediment the liposomes. RPE65 cosedi-sive, vesiculated smooth endoplasmic reticulum where mented with the liposome pellet. RPE65 also cosedi-the enzymes involved in visual cycle retinoid metabomented with synthetic dipalmitoyl-, 1-palmitoyl, 2-do-lism and isomerization are localized; and the outer seg-

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Section: Discussionmentioning

confidence: 99%

RPE65, the Major Retinal Pigment Epithelium Microsomal Membrane Protein, Associates with Phospholipid Liposomes

Tsilou

Hamel

et al. 1997

Archives of Biochemistry and Biophysics

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“…An SLM can be assembled on silane-derivatized silica surfaces [6,7] or thiol-coated gold surfaces [8][9][10][11][12], or it can be obtained by the covalent linking of chemically modified phospholipids with silica [13] or gold. Another type of lipid monolayer, also called immobilized artificial membrane (IAM), can be obtained by the direct covalent bonding of phospholipid analogs on silica particles [14,15]. Such lipid/membrane systems are geometrically well defined and are potentially suitable for the aforementioned quantitative studies.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of supported lipid bilayer deposition from vesicle suspensions

Bucak

Wang

Laibinis

et al. 2010

Journal of Colloid and Interface Science

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“…The resulting phospholipid layer reproduces the lipid outer sheet of a biological cell membrane. Biopartitioning potential of IAM chromatography has proved remarkably effective for rapid purification of functional membrane proteins (69) and predicts the phospholipophilicity and potential membrane permeability of small drugs (70). As an alternative approach to measure lipophilicity parameters, IAM retention can replace liposomes by facilitating membrane simulation for rapid permeability measurements and minimizing the difficulties associated with liposome-water partitioning (71).…”

Section: Predictive Non-cell Based Modelsmentioning

confidence: 99%

In Vitro Cerebrovascular Modeling in the 21st Century: Current and Prospective Technologies

et al. 2014

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The blood-brain barrier (BBB) maintains the brain homeostasis and dynamically responds to events associated with systemic and/or rheological impairments (e.g., inflammation, ischemia) including the exposure to harmful xenobiotics. Thus, understanding the BBB physiology is crucial for the resolution of major central nervous system CNS) disorders challenging both health care providers and the pharmaceutical industry. These challenges include drug delivery to the brain, neurological disorders, toxicological studies, and biodefense. Studies aimed at advancing our understanding of CNS diseases and promoting the development of more effective therapeutics are primarily performed in laboratory animals. However, there are major hindering factors inherent to in vivo studies such as cost, limited throughput and translational significance to humans. These factors promoted the development of alternative in vitro strategies for studying the physiology and pathophysiology of the BBB in relation to brain disorders as well as screening tools to aid in the development of novel CNS drugs. Herein, we provide a detailed review including pros and cons of current and prospective technologies for modelling the BBB in vitro including ex situ, cell based and computational (in silico) models. A special section is dedicated to microfluidic systems including micro-BBB, BBB-on-a-chip, Neurovascular Unit-on-a-Chip and Synthetic Microvasculature Blood-Brain Barrier.

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Immobilized artificial membrane chromatography: Rapid purification of functional membrane proteins

Cited by 54 publications

References 34 publications

RPE65, the Major Retinal Pigment Epithelium Microsomal Membrane Protein, Associates with Phospholipid Liposomes

RPE65, the Major Retinal Pigment Epithelium Microsomal Membrane Protein, Associates with Phospholipid Liposomes

Dynamics of supported lipid bilayer deposition from vesicle suspensions

In Vitro Cerebrovascular Modeling in the 21st Century: Current and Prospective Technologies

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