Immobilized bacterial α-amylase for effective hydrolysis of raw and soluble starch

Gangadharan, Dhanya; Nampoothiri, K. Madhavan; Sivaramakrishnan, Swetha; Pandey, Ashok

doi:10.1016/j.foodres.2009.02.008

Cited by 54 publications

(54 citation statements)

References 23 publications

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“…The enzyme was eluted with a linear gradient of KCl (0. 1 2 M) at a flow rate 0.5 mL/min. Fractions with amylase activity obtained by ion-exchange chromatography were mixed and concentrated.…”

Section: Purification Of Amylasesmentioning

confidence: 99%

“…A wide variety of microbes are known to produce α-amylases [1]. Usually α-amylases (EC 3.2.1.1) hydrolyze α-1,4 glycosidic linkages in starch [2], yielding different sorts of oligo and monosaccharides [3].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, immobilized cells are advantageous over free cells for their better operational stability, higher efficiency of catalysis, continuous operation over a prolonged time period, increased productivity, and reusability [1,9]. In this scenario, selection of a proper technique and supporting material is very important and can increase the efficiency of the immobilized cells.…”

Section: Introductionmentioning

confidence: 99%

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Isozymes of α-amylases from newly isolated Bacillus thuringiensis CKB19: Production from immobilized cells

Maity

Samanta

Halder

et al. 2011

Biotechnol Bioproc E

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The aim of this study was to produce two isozymes of α-amylase by immobilization of a newly isolated soil bacterium. The bacterium was identified as Bacillus thuringiensis CKB19 on the basis of its 16S rRNA profile. Enzyme production by free cells increased linearly with cell growth up to 34 h in starch containing enriched liquid media. The active bacterial cells were immobilized in Caalginate beads, and operational stability of the entrapped cell was optimized for amylase production. Enzyme production was optimal at an alginate concentration of 2 g% (w/v), calcium chloride concentration of 1 M, and with 300 beads (each bead contained 2 × 10 7 cells)/250 mL flask. Amylase production by the immobilized cells was about 3 times higher than free cell fermentation after 34 h of incubation. It was observed that the immobilized bacterium secreted two different amylases (Am-I and Am-II) into the culture fluid. The molecular masses of Am-I and Am-II were 59.6 and 44.7 kd, respectively, and showed optimum activity at pH 5.0 and 9.0. Both amylases showed optimum activity at 40 o C and were stable at the same temperature, with losses of only 10 and 20% (for Am I and Am II, respectively) of their original activities after 24 h of incubation. Further, both amylases were salt tolerant (up to 4 M NaCl) and hydrolyzed raw starchy foods into glucose. All these characteristics make this enzyme mixture suitable for use as a digestive aid and for the improvement of digestibility of animal feed ingredients.

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Section: Purification Of Amylasesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isozymes of α-amylases from newly isolated Bacillus thuringiensis CKB19: Production from immobilized cells

Maity

Samanta

Halder

et al. 2011

Biotechnol Bioproc E

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show abstract

“…However, some disadvantages are often associated with this carrier, including high biomolecule leakage, low mechanical strength and large pore size [12,13]. In order to optimize the encapsulation efficiency and control release of enzyme from the gel matrix, various methods have been proposed [14][15][16][17][18][19], including covalent cross-linking with polymers, such as poly(acrylic acid) and chitosan, and coating the surface of alginate gel beads with other reagents, such as poly-l-lysine and glutaraldehyde. Matto et al [14] have employed calcium alginate-starch hybrid gel as an enzyme carrier both for surface immobilization and entrapment of bitter gourd peroxidase.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate–chitosan beads

Zhou

2010

International Journal of Biological Macromolecules

108

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“…The extent of lactose hydrolysis whey processing, skimmed milk production, etc. has been greatly enhanced using respective enzymes as immobilized forms (Carpio et al 2000;Oh 2007;Gangadharan et al 2009). Biodiesel has gained importance in the recent past for its ability to replace fossil fuels which are likely to run out within a century (Iso et al 2001;Antolin et al 2002;Tiwari et al 2007;Dizge and Keskinler 2008;Jegannathan et al 2008;Yagiz et al 2007;Canakci and Gerpen 2003).…”

Section: Introductionmentioning

confidence: 99%

The Mathematical Theory of Diffusion and Reaction in Enzymes Immoblized Artificial Membrane. The Theory of the Non-Steady State

Ramanathan¹,

Rasi²,

Rajendran³

2015

J Membrane Biol

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In this paper, mathematical model pertaining to the decomposition of enzyme-substrate complex in an artificial membrane is discussed. Here the transport through liquid membrane phases is considered. The model involves the system of non-linear reaction diffusion equations. The non-linear terms in this model are related to Michaelis-Menten reaction scheme. Approximate analytical expressions for the concentrations of substrate and product have been derived by solving the system of non-linear reaction diffusion equations using new approach of homotopy perturbation method for all values of Michaelis-Menten constant, diffusion coefficient, and rate constant. Approximate flux expression for substrate and product for non-steady-state conditions are also reported. A comparison of the analytical approximation and numerical simulation is also presented. The results obtained in this work are valid for the entire solution domain.

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Immobilized bacterial α-amylase for effective hydrolysis of raw and soluble starch

Cited by 54 publications

References 23 publications

Isozymes of α-amylases from newly isolated Bacillus thuringiensis CKB19: Production from immobilized cells

Isozymes of α-amylases from newly isolated Bacillus thuringiensis CKB19: Production from immobilized cells

Immobilization of Saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate–chitosan beads

The Mathematical Theory of Diffusion and Reaction in Enzymes Immoblized Artificial Membrane. The Theory of the Non-Steady State

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