Immobilizing cholesterol oxidase in chitosan–alginic acid network

Immobilization of cholesterol oxidase on magnetic fluorescent core-shell-structured nanoparticles

“…The thermal, pH and storage stabilities of COD were increased by immobilization. Yapar et al immobilized COD in conducting network via complexation of chitosan with alginic acid [3]. The immobilized COD still maintained 63% of its initial activity after 44 measurements, showing an excellent operational stability.…”

Application of chitosan beads immobilized Rhodococcus sp. NCIM 2891 cholesterol oxidase for cholestenone production

“…Cholesterol oxidase has been immobilized in several support materials [11,[23][24][25][26][27][28][29][30][31][32][33][34]. However, more focus has recently been laid on the use of biocompatible, biodegradable and low cost materials such as, chitosan to immobilize enzyme for industrial applications [32,35,36]. The success of an immobilization method largely depends on the nature of the support material and the reaction environment being used [37,38].…”

Development of polyampholyte hydrogels based on laponite for electrically stimulated drug release

“…PAHs are often referred to as smart or intelligent gels due to the reversible deformations such as swelling, deswelling, bending, rotating or translating when an external stimuli is applied . Synthetic polymers having ionic side groups, such as poly(acrylic acid), polyethyleneimine, poly(methacrylic acid), poly(3‐acrylamidopropyltrimethylammonium chloride), poly(dimethylaminoethyl methacrylate), poly(vinyl‐2‐aminoethyl ether), poly(acryloyloxyethyltrimethylammonium chloride) and poly(2‐acrylamido‐2‐methylpropanesulfonic acid), and natural polymers such as gelatine, chitosan, gellan gum, hyaluronic acid, hydroxyethyl cellulose and dextran are widely used in preparing PAHs. Also, introducing the network structure of clay particulates such as laponite (Lap) bearing silanole groups may be a good approach for forming PAHs.…”